Báo cáo khoa học: Mutational analysis of the C-domain in nonribosomal peptide synthesis pot

... further efforts were made to track down the actual target of the inhibitors. DISCUSSION The purpose of t his work was to identify the key residues in the C-domain a nd their potential role in the ... identify their target in the C-domain using peptide mapping. T hus the inhibitory effect may be due to interference with other reactive residues in NRPS modules...

Ngày tải lên: 17/03/2014, 11:20

... Three of them (CRB-3–5) are in the vicinity of two putative coiled-coil domains, which suggests that they may participate together with the coiled-coil domains in folding of the N-terminal half of ... feature of Mrs2p is the existence of two transmembrane-domains and a short connect- ing sequence of about 7–8 amino acids. This is sup- posed to be the only part of...

Ngày tải lên: 23/03/2014, 10:21

... domain swapping mechanism involving the core and COOH-terminal domains of the two subunits. We hypothesized that the shortened linker in the mutant enzyme destabilized the interaction between the ... important, then it is likely that the binding involves an interaction with two regions of DNA at the point of crossing. One hypothesis to explain how the enzyme provides...

Ngày tải lên: 29/03/2014, 22:21

... linkage of the active site Ser of the protease to the carboxyl group of P 1 by an ester bond and insertion of the N-terminal part of the RCL as strand 4 in b-sheet A (s4A) of the serpin. Consequently, the ... behaviour of the serpin. Complex formation between serpins and their cognate proteases is fuelled by the thermodynamic properties of the serpin. Acc...

Ngày tải lên: 20/02/2014, 11:20

... intermediate of NO synthesis [26]. On the other hand, in contrast with Asn130 in arginase I, the corres- ponding Asn149 of arginase II was not found to be a ligand for the a-carboxylate group of the transition state ... ligand for the a-carboxyl group of arginine in arginase I [9,23,24]. On this basis, direct repulsion of the negatively charged Asp130 would expla...

Ngày tải lên: 16/03/2014, 12:20

... FEBS 4773 of Q A – in PSII centers that are affected by the nonsatu- rating concentration of inhibitor [7,8]. The E m of Q A is raised in the presence of DCMU in the Q B pocket, making it energetically ... to the reduction of the PQ pool. Results As reported in the literature, the I step of the O–J–I–P fluorescence transient cannot be clearly distinguished...

Ngày tải lên: 19/02/2014, 05:20

... C-terminal region, including the PAS-B domain of BMAL1, is required for the binding to DEC1, Fig. 4. Identification of the domain of D EC1 that interacts with BMAL 1. Interactions of various DEC1 ... region of DEC1, including the bHLH domain, interacted with the C-terminal r egion o f BMAL1 in a mammalian two-hybrid assay. Accordingly, a Fig. 5. Analysis of DEC1 mutan...

Ngày tải lên: 19/02/2014, 16:20

... structure of the N-terminal domain of the human homologue, solved at 2.2 A ˚ resolution [11]. The obtained model covers residues 254–568 of HpPex5p, corresponding to the C-terminal half of the protein ... coordinates are available from the authors upon request. Addition of the L2 peptide was possible due to the conserved binding site and the presence of a YQSKL...

Ngày tải lên: 21/02/2014, 00:20

... flavin-binding domain of ferre- doxin : NADP + reductase [10]. The lysine residue is not conserved; instead, an arginine residue interacts with the FAD molecule of the enzyme. In the case of FMN-binding ... Blanche F (1995) Purification of the two-enzyme sys- tem catalyzing the oxidation of the d-proline residue of pristinamycin IIB during the last step of pristi...

Ngày tải lên: 07/03/2014, 02:20

... (two cDNAs). Initial mapping of the region of PP2Ac involved in TIPRL binding was obtained from the cDNAs that showed positive interaction with TIPRL. The extension of these cDNAs is shown in Fig. ... with GST–a4 in the presence of His–PP2Aca (data not shown). Similar results were obtained using the PP2Ac-binding domain of a4, a4D222 [24], instead of the full-len...

Ngày tải lên: 07/03/2014, 05:20