Báo cáo Y học: Oxidation of propionate to pyruvate in Escherichia coli Involvement of methylcitrate dehydratase and aconitase pot
... (2001) In vitro con- version of propionate to pyruvate by Salmonella enterica enzymes: 2 -methylcitrate dehydratase (PrpD) and aconitase enzymes cata- lyze the conversion of 2 -methylcitrate to 2-methylisocitrate. Biochemistry ... reconstitution of the oxidation of propionyl-CoA to pyruvate by the use of purified PrpC, PrpD, AcnB and PrpB from E. coli. PrpD...
Ngày tải lên: 17/03/2014, 10:20
... Proline, hydroxyproline and hydroxylysine were examined at 254 nm by reverse-phase HPLC of their phenylisothiocarbamate derivatives [26], as reported previously [20]. The serine, threonine and tyrosine contents ... from anion-exchange (AE) HPLC of water-soluble matrix (WSM) and EDTA-soluble matrix (EDTA-SM). Cysteine, hydroxylysine, hydroxy- proline, phosphoserine, proline and try...
Ngày tải lên: 21/02/2014, 01:21
... confirmed by probing cellular extracts with an antibody against the flavin moiety of covalently flavinylated proteins (a-FAD). Following western blotting analysis, a band Fig. 2. (A) Succinate dehydrogenase ... activity of mitochondrial riboflavin trans- port system(s) and two enzymes, riboflavin kinase (EC 2.7.1.26) and FAD synthetase (EC 2.7.7.2), which catalyze the synthesis of FMN a...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: Enhancing the protein production levels in Escherichia coli with a strong promoter potx
... solubility of a protein, such as folding velocity and hydrophobicity. When proteins are produced, the synthesis rate of the protein may affect the proportion of soluble protein. Previously, it was ... Jenny Ottosson and Sophia Hober School of Biotechnology, Department of Proteomics, Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden Introduction Recom...
Ngày tải lên: 06/03/2014, 01:20
Báo cáo y học: "A Practical Approach to Management of Chronic Hepatitis B"
... b b i i o o g g r r a a p p h h y y Ke-Qin Hu, MD, is the Director of Hepatology Services and Associate Professor of Clinical Medicine, Divisions of Gastroenterology and Transplantation, University of California, Irvine, ... treatment [16]. 4. Indications of HBV Treatment Historically, levels of HBV DNA and ALT, and histological activity of liver biopsy have b...
Ngày tải lên: 03/11/2012, 09:41
Tài liệu Báo cáo Y học: Oxidation of phenols by laccase and laccase-mediator systems doc
... N-hydroxyphtalimide (HPI) and 2,2,6,6- tetramethylpiperidin-1-yloxy free radical (TEMPO) as mediators, since they greatly differ both in their mechan- ism of action and in their specificity. In ... Roma, Italy To investigate how solubility and steric issues affect the laccase-catalysed oxidation of phenols, a series of oligomeric polyphenol compounds, having increasing siz...
Ngày tải lên: 21/02/2014, 01:21
Báo cáo Y học: Human bile salt-stimulated lipase has a high frequency of size variation due to a hypervariable region in exon 11 pot
... fat-soluble vitamin esters and cholesterol esters in the small intestine. In some species, including humans, the gene is also expressed in the lactating mammary gland and the resulting protein is a constituent of ... exocrine pancreas in all species examined. BSSL has a broad substrate speci®city a nd contributes to the hydrolysis of dietary mono-, di-, a nd tri-acylglycerol...
Ngày tải lên: 24/03/2014, 03:21
Báo cáo y học: "Pre-hospital intubation by anaesthesiologists in patients with severe trauma: an audit of a Norwegian helicopter emergency medical service"
... included the type of airway device and drugs used to facilitate ETI, complications and the use of monitoring, including capnography. We anonymised the involved HEMS physicians and recorded them ... conse- quences of delaying ETI until arrival in the ED. A recent study from the Netherlands [20] also showed a failure to adhere to guidelines for pre-hospital ETI in traumati...
Ngày tải lên: 25/10/2012, 09:56
Báo cáo y học: "Maternal Outcomes According to Placental Position in Placental Previa"
... According to Placental Position in Placental Previa Dong Gyu Jang, Ji Sun We, Jae Un Shin, Yun Jin Choi, Hyun Sun Ko, In Yang Park, Jong Chul Shin Department of Obstetrics and Gynecology, ... hemoglobin level of prior to surgery, 1 day after surgery, and 3 days after surgery, the amount of transfusion during surgery, estimated blood loss during operation, pla- cent...
Ngày tải lên: 25/10/2012, 10:56
Báo cáo y học: "MALDI-TOF MS Combined With Magnetic Beads for Detecting Serum Protein Biomarkers and Establishment of Boosting Decision Tree Model for Diagnosis of Colorectal Cancer"
... useful tool for integrating separation and analysis of complex mixtures of proteins. Captured proteins are then analyzed by TOF-MS, generating a spectral map depicting approximations of the ... may also be involved. In conclusion, MALDI-TOF MS combined with magnetic beads is one useful tool for integrating se- paration and analysis of complex mixtures of pro- teins. With th...
Ngày tải lên: 25/10/2012, 11:18