Báo cáo Y học: Trehalose-phosphate synthase of Mycobacterium tuberculosis Cloning, expression and properties of the recombinant enzyme pdf
... expression and properties of the recombinant enzyme Y. T. Pan 1 , J. D. Carroll 2 and A. D. Elbein 1 1 Department of Biochemistry and Molecular Biology and 2 Department of Microbiology and Immunology, ... amounts of the recombinantTPS.Afteranincubationof15min,onesetofincubation mixtures was assayed by the anthrone colorimetric method and the other set was...
Ngày tải lên: 17/03/2014, 10:20
... Interconversion of trehalose and maltose (Eur. J. Biochem. 271) 4269 Trehalose synthase of Mycobacterium smegmatis Purification, cloning, expression, and properties of the enzyme Yuan T. Pan 1 , ... available. Assay of trehalose synthase activity The enzymatic activity of TreS was routinely measured by determining the formation of reducing sugar when enzyme...
Ngày tải lên: 23/03/2014, 13:20
... which limits the ability of the nucleus to resorb additional water and reduces stiffness of the disc (3,6,7). This hypothetically results in a more even distribution of weight across the in- tervertebral ... Mork 2,3 , Morgan Pyne 4 and Ryan A. Baker 4 1. Chief of Pain Medicine and Anesthesiology 2. Chief of Spine Surgery 3. MicroSpine, DeFuniak Springs, FL...
Ngày tải lên: 26/10/2012, 09:07
Báo cáo y học: "Low socio-economic status, smoking, mental stress and obesity predict obstructive symptoms in women, but only smoking also predicts subsequent experience of poor health"
... The results of the study suggest that life-style factors such as mental stress, obesity and smoking among women are related to airway symptoms and also quality of life many years later. Key ... are scarce. The results of the study suggest that life-style factors such as mental stress, obesity and smoking among women are related to symptoms in the airway and also q...
Ngày tải lên: 31/10/2012, 15:34
Báo cáo y học: "Elevated plasma homocysteine is positively associated with age independent of C677T mutation of the methylenetetrahydrofolate reductase gene in selected Egyptian subjects"
... Frequency of the MTHFR genotypes and the result of the Chi square testing in the different studied groups. Showing the frequency and the results of the cross tabulation of the T allele and the different ... mutation. The wild type CC shows a single band at the 198 base pair (Bp). The heterozygote CT showed two bands, one at the 198 and the other...
Ngày tải lên: 03/11/2012, 09:49
Tài liệu Báo cáo Y học: A novel, inducible, citral lyase purified from spores of Penicillium digitatum docx
... is approximately the monomeric size of these hydratase/aldolase enzymes. All of these enzymes exhibit more then 75% of their maximum activity at pH 7.6, the optimum pH of citral lyase [27,30–32]. ... as the standard. RESULTS Induction of citral lyase Initially, the reproducibility of the results was hindered by variations in the citral lyase activity of the P. digi...
Ngày tải lên: 21/02/2014, 01:21
Báo cáo Y học: Guanosine diphosphate-4-keto-6-deoxy-D-mannose reductase in the pathway for the synthesis of GDP-6-deoxy-D-talose in Actinobacillus actinomycetemcomitans potx
... GDP-4-keto-6-deoxy- D -mannose reductase and the stereoselectivity of the reduction deter- mines the direction of synthesis of these two 6-deoxyhex- oses. However, neither the gene encoding the biosynthesis of GDP-6-deoxy- D -talose ... obtained from the hydrolysate of the purified GDP-6- deoxytalose. (A) Glycosides of the purified serotype a-specific poly- sacchari...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo Y học: Agmatine oxidation by copper amine oxidase Biosynthesis and biochemical characterization of N-amidino-2-hydroxypyrrolidine pdf
... · 10 )1 M phosphate buffer) and 25.0 °C (Fig. 1). Values of k cat and K 0 m are independent of the enzyme assay. Biosynthesis of N -amidino-2-hydroxypyrrolidine N-Amidino-2-hydroxypyrrolidine was synthesized as ... n the role played by the product(s) of agmatine metabolism on cell function(s). Here, the b iosynthesis and the biochemical characterization of N-amidin...
Ngày tải lên: 17/03/2014, 23:20
Báo cáo Y học: A new high affinity binding site for suppressor of cytokine signaling-3 on the erythropoietin receptor potx
... modelled the complex of the SOCS-3 SH2 domain and the receptor peptides pY401 and pY429pY431 (Fig. 6). For comparison, Fig. 2. Comparison of SOCS-3 binding to pY343, pY401 and pY429pY431 of the human ... G53V, L58A, L93A and R94E with the peptide pY429pY431 (pYpY). (B) Precipitation of SOCS-3 WT or SOCS-3 R94E with the peptides pY429pY431 (pYpY), pY429F431 (pYF) a...
Ngày tải lên: 24/03/2014, 00:21
Báo cáo Y học: Human bile salt-stimulated lipase has a high frequency of size variation due to a hypervariable region in exon 11 pot
... sequence of t he tail. The f unction of the tail has previously been discussed in the literature. Deletion of the tail by in vitro mutagenesis of the human enzyme was shown to signi®cantly decrease expression ... pancreatic BSSL [15]. On the other hand, we and others have shown that t he repeats are completely dispensable for the typical functional properties of...
Ngày tải lên: 24/03/2014, 03:21