Báo cáo khoa học: Identification of sodium salicylate as an hsp inducer using a simple screening system for stress response modulators in mammalian cells pptx

... examined the effects of SA on stress response in mammalian cells using a simple screening system, and revealed that SA is a potent Hsp inducer in mammalian cells, thereby protecting cells against ... Identification of sodium salicylate as an hsp inducer using a simple screening system for stress response modulators in mamma...

Ngày tải lên: 17/03/2014, 10:20

... nondenaturing and transverse urea gradient PAGE, and activity was assessed against tPA [6]. Complex formation assays Wild-type and Ser52Arg neuroserpin were incubated i n various ratios with tPA at ... faster rate, in keeping with the more severe clinical phenotype. The P ortland mutant h as a normal unfolding transition in urea and a normal melting temperature but is inactive as...

Ngày tải lên: 07/03/2014, 16:20

... C), for pGEX–EWS; EAD forward d(CGG AAT TCA TGG CGT CCA CGG ATT ACA G) and EAD reverse d(CGC TCG AGT CAT CCG GAA AAT CCT CCA GAC T), for pGEX–EAD; RGG1 forward d(CGG AAT TCC CAG GAG AGA ACC GGA ... G-quadruplex DNA. (A) EMSA was performed with EWS (lanes 2 and 4) and 32 P-labeled ETS-1 (lanes 3 and 4) or ssDNA L (lanes 1 and 2). (B) EMSA was per- formed with EWS (lanes 2, 4 and 6) and 32...

Ngày tải lên: 15/02/2014, 01:20

... AAG CCG ATG ACA CCA ATT (asd sense) This study asd2 GCA GGT TCA TAG TGC ATG (asd antisense) This study Kan RP GGT GCG ACA ATC TAT CGA (kanamycin sense) [19] Kan FP CTC ATC GAG CAT CAA ATG (kanamycin ... Catlin BW (1990) Branhamella catarrhalis: an organism gaining respect as a pathogen. Clin Microbiol Rev 3, 293–320. 2 Karalus R & Campagnari A (2000) Moraxella catarrh- alis: a r...

Ngày tải lên: 18/02/2014, 14:20

... TGACCAAGGCAACAGAACCA AATCAGACGGCCGGTATGTG Heat shock 70 kDa protein 1 2A CGAAAAAGGACAGCAGTTGAAA CTCATCCTCCACCGGATTGT HSP2 3 CGTCCGATTTCTTCTCGTGTTT ACCAGAAGACATTACAGTGAAAATTGA Chaperonin-containing ... 7, isoform b, isoform 1 GGGAACCAGCAGTCGTCAAA CGTCCACTGAGAGGATGAGACA Inhibitor of kappa light polypeptide enhancer in B cells, kinase complex-associated protein AAAGCAGAGCAGAAAAAGTGGAA GGA...

Ngày tải lên: 18/02/2014, 16:20

... by invertebrates, and has three crystal phases: calcite, ara- gonite and vaterite. Although calcite is the most stable crystal thermodynamically, many organisms can form metastable aragonite crystals ... cDNA and DNA encoding OMM-64 To obtain cDNA clones encoding proteins contained in the HMW aggregate, immunoscreening was performed using an antiserum that reacts mainly with the aggrega...

Ngày tải lên: 18/02/2014, 17:20

... cerevisiae strain GIL77 Two oligo DNAs (5¢-CTTCGTCGACAAGATGTGGAG GTTGAAGATA-3¢ and 5¢-GTCCGCTAGCTCAAGGCA AAGGAACTCTTCT-3¢), corresponding to the N- and C-terminal sequences of b-amyrin synthase from ... struc- tures of soyasapogenol A, soyasapogenol B, and soyasapogenol E, oleanene sapogenols from soybean, structures of soyasaponin I, soyasaponin II, and soyasa- ponin III. Chem Pharm B...

Ngày tải lên: 19/02/2014, 07:20

... on a functional STAT2 DNA binding domain do not appear to play an important role in mediating the transcription of these genes. The c-fos gene was examined in this system as an example of a GAS-driven ... VV-II cells using relative quantitative real-time PCR as for Fig. 2. For each sample, b-actin was evaluated as a reference gene and used for normaliza- ti...

Ngày tải lên: 19/02/2014, 07:20

... The main fraction contained a 75 kDa matriptase as a major component and a few contamin- ating proteins as analyzed by SDS ⁄ PAGE. RNAi experiments with OVISE cells Matriptase siRNAs and a scrambled ... 5¢-GGAUCAAAGAGAACACUGGGGUAUA-AG-3¢; and #1513 sense, 5¢-AGUUCACGUGCAAGAACAAG UUCUG-AG-3¢. The forward sequence of the scrambled RNA was 5¢-GAUCCAAGUAAUACAGAGAUGGGAG AG-3¢. OVIS...

Ngày tải lên: 19/02/2014, 07:20

... ACCACTTTGTACAAGAAAGCTGGGT yef1hisf CAATAAATCTGCTTACGTGACATTTTTTACTAAAAGAGAAT ATGCGTACGCTGCAGGTCGAC yef1hisr GAACCCTTGACTACGGAAACGCAGGATGTGGGAAATCG TTAATCGATGAATTCGAGCTCG pos5hisf CATAAATAAAAGGATAAAAAGGTTAAGGATACTGATTAAA ATGCGTACGCTGCAGGTCGAC pos5hisr CTTAGAGAATCTCATTGAATCTTTGCATTCAGAGCGT TTAATCGATGAATTCGAGCTCG pos5leu21.6f CATAAATAAAAGGATAAAAAGGTTAAGGATACTGATTAAA ATGCCAATTCTGTGTTTCCCGGAAATG p...

Ngày tải lên: 20/02/2014, 01:20