Báo cáo khoa học: Structural characterization of the lipopolysaccharide O-polysaccharide antigen produced by Flavobacterium columnare ATCC 43622 potx

... of note that the structure of the LPS O -antigen of F. columnare differs structurally from the LPS O -antigen of the fish pathogen Flavobacterium psychrophilium [21], which is a linear polymer of ... Structural characterization of the lipopolysaccharide O -polysaccharide antigen produced by Flavobacterium columnare ATCC 43622 Leann L. MacLean 1 ,...

Ngày tải lên: 17/03/2014, 10:20

... for the p eptide. NMR s pectroscopy was further utilized to explore the structural properties of Nogo-40. The very narrow reson- ance dispersion of amide protons ( 0.7 p.p.m) and the lack of ... Zn 2+ . Furthermore, the d etermination of the Nogo-40 solution structure offers a starting point for further understanding the interaction between NgR and Nogo-40, and for fu...

Ngày tải lên: 16/03/2014, 16:20

... for formation of short helices, transiently, and the chain as such is highly dynamic. Structure of the core of the oligomers Because of the large size of the core of the oligomer, the NMR spectra ... characteristics of the individual domains has the potential to throw valuable light on the interac- tions of the individual domains, and the mechanism and...

Ngày tải lên: 23/03/2014, 11:20

... regulated by the binding of extrinsic calmodulin (CaM). The c subunit is the catalytic subunit and the d subunit is an intrinsic mole- cule of CaM that binds to the enzyme even in the absence of Ca 2+ [2]. ... even in the absence of Ca 2+ . Studies on the disruption of the holoenzyme produced two functional complexes, the acd complex and the cd complex dem-...

Ngày tải lên: 19/02/2014, 17:20

... Hep Hep b | Hex 2 -Hex-Hep-AnKdo-ol | Hep | Hep a Major isomer of the Hex3 glycoform (estimated from the intensity of the fragments in MS 2 experiments). b Minor isomer of the Hex3 glycoform (estimated from the intensity of the fragments in MS 2 experiments). Fig. ... residue, probably owing to the partial occurrence of PEtn attached to the phosphate group at O-4 of...

Ngày tải lên: 08/03/2014, 02:21

... D1, and the extrinsic proteins of the oxyge n-evolving complex the 33 k Da, cyt c 550 and 12 k Da. The presence of the cyt c 550 in the PSII fraction w as confirmed by h eme staining of the g el ... analysis of the side-view projections shows a maximal thickness of the PSII complex of about 9 nm including the densities on the lum- enal surface that has been...

Ngày tải lên: 16/03/2014, 18:20

... K d is the equilibrium dissociation constant of the 5¢ exon mimic, h is the fraction of 5¢ exon mimic bound to the ribozyme, and [ribozyme] u is the concentration of unbound ribozyme in the reaction. Measurement ... 15%. The solid line represents the log-linear increase in the data set from pH of 5–7 (slope = 0.5 ± 0.03). The extrapolation of the line to pH 7.5...

Ngày tải lên: 18/02/2014, 18:20

... than the other helices. These equilibria also occur in the other two helices, as shown by the exchange pattern [26], although they are less well-ordered, as judged by the lower S 2 . The types of ... (c) the degree of restriction of these fast internal motions (which is measured by the square of the order parameter, S 2 ). Thus, in residues where the relax- ati...

Ngày tải lên: 07/03/2014, 06:20

... identified by the mass increase of 42 mass units. The relative level of acetylation of a peptide was estimated on the basis of the intensity ratio of the native and acetylated species. From the data ... of charges measured by electrospray MS. Theoretical mass is the mass calculated on the basis of the protein amino-acid sequence. The relative abundance ref...

Ngày tải lên: 07/03/2014, 14:20

... Switzerland The fidelity of protein synthesis depends on the correct attachment of amino acids to the 3¢-ends of their cog- nate tRNA species by aminoacyl-tRNA synthetases (aaRS), a family of enzymes ... diminished Fig. 5. Model of the acceptor end of tRNA Ser bound in the active site of mMbSerRS. The view is focused on the acceptor part of tRNA in the active...

Ngày tải lên: 16/03/2014, 06:20