Báo cáo khoa học: Purification and characterization of NTPDase1 (ecto-apyrase) and NTPDase2 (ecto-ATPase) from porcine brain cortex synaptosomes doc

... cytochrome domain and approaches one of propionates of heme, was mutated to Tyr, and the thermodynamic and kinetic properties of the mutant (F166Y) were compared with those of the wild-type (WT) ... Masahiro Samejima 1 and Takeshi Nishino 3 1 Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan 2 Departm...

Ngày tải lên: 19/02/2014, 18:20

... Pro, Val or Lys, c-carboxylation of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different residues. It ... 1977 effect of the d-Phe on structure, was also studied. The unusual structure of conomarphin further demon- strates the high diversity of conotoxins. Results Purification and p...

Ngày tải lên: 18/02/2014, 17:20

... & Kwon YM (2001) cDNA cloning of two isoforms of ornithine carbamoyltransferase from Canavalia lineata leaves and the effect of site-directed mutagenesis of the carbamoyl phosphate binding ... This paper reports the purification and characterization of GAT from wild watermelon leaves, and discusses its function during drought ⁄ strong-light stress on the basis of...

Ngày tải lên: 20/02/2014, 03:20

... Sarris and Palade [30] and Schauer [6]. A solution of 750 lL of glycoprotein (5 mgÆmL )1 ) was added to 250 lLof0.04 M of NaOH, vortexed, incubated on ice for 45 min and neutralized with 1 mL of ... sialidasetypeX,protease enzymes and molecular mass standards were purchased from Sigma. Preparation of crab sera Freshwater field crabs, Paratelphusa jacquemontii were collected...

Ngày tải lên: 21/02/2014, 00:20

... 1899 absorption maxima at 420 nm (c band), 530 nm (b band) and 557 nm (a band) are characteristic of cytochrome b. Heme was extracted from the protein with acidic acetone and the pyridine hemochrome spectrum ... FEBS Lett. 457 , 291–297. 1904 G. J. Mander et al.(Eur. J. Biochem. 269) Ó FEBS 2002 Purification and characterization of a membrane-bound enzyme complex from the sul...

Ngày tải lên: 21/02/2014, 03:20

... min, bands with molecular masses of 43 and 39 kDa were observed in addition to undigested bands of ZP2 and ZP3. After incubation for 10 min, three major bands with molecu- lar masses of 43, 39 and ... to Professor F. S. Howell (Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Tokyo) for reading the manuscript and to Dr K. Y...

Ngày tải lên: 07/03/2014, 04:20

... provided by a grant to T. Kyndt from the ‘Institute for the Promo- tion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen)’ and by FWO-Vlaanderen (project number 3G005100). ... Isolation and charac- terization of the cysteine-proteinases from the latex of Carica candamarcensis Hook. Biol Chem Hoppe-Seyler 374, 501–506. 18 Walraevens V, Vandermeers-Piret...

Ngày tải lên: 07/03/2014, 11:20

... faint 28 kDa band and additional bands were also detected. A clear 28 kDa band and addi- tional bands were observed from cotyledons obtained 5 days after imbibition. The identity of the 28 kDa protein ... grade. Plants Fresh sprouts of daikon radish (R. sativus) grown by hydro- ponics were purchased from a grocery store and used for purification of the enzyme. For analysis o...

Ngày tải lên: 16/03/2014, 04:20

... was stopped by the addition of 750 lL of acetic acid, and the color developed by the addition of 250 lL of ninhydrin (4 mgÆmL )1 )at 95 °C for 1 h. A standard curve of different ornithine concentrations ... (1994) Purification and characterization of arginine amidinohydrolase from Bacillus brevis TT02-8. Biosci. Biotechnol. Biochem. 58, 1045–1049. 27. Soru, E. (1983) Chem...

Ngày tải lên: 16/03/2014, 16:20

... Purification and characterization of NTPDase1 (ecto-apyrase) and NTPDase2 (ecto-ATPase) from porcine brain cortex synaptosomes Filip Kukulski and Michal Komoszyn ´ ski Department of Biochemistry, ... Biochemistry, Institute of General and Molecular Biology, N. Copernicus University, Torun ´ , Poland We purified to homogeneity and characterized NTPDase1...

Ngày tải lên: 17/03/2014, 10:20