Báo cáo khoa học: Deadenylation of interferon-b mRNA is mediated by both the AU-rich element in the 3¢-untranslated region and an instability sequence in the coding region pot

... Deadenylation of interferon-b mRNA is mediated by both the AU-rich element in the 3¢-untranslated region and an instability sequence in the coding region Muriel Paste´, Georges Huez and ... However, the deadenylation and degradation of IFN-b mRNA is under the control of twoindependent elements, one of which is located in the mRNA coding region. The coexistence of two independent ... genecontained or not the ARE (pIFNHA and pIFNHAAU–). In addition, the sequence encoding the HA epitope wasinserted at the end of the IFN-b coding sequence todistinguish the products resulting...

Tài liệu Báo cáo khoa học: Modulation of F0F1-ATP synthase activity by cyclophilin D regulates matrix adenine nucleotide levels pptx

... approaching the reversal potential of the ANT [23]. At 0 mV, during which both the ANT and the F0F1-ATP synthase operate in reverse mode, the increase in ADP–ATP exchange rate mediated by the ANT ... an insigniﬁcant increase (1.38–1.7%) in the ADP–ATPexchange rate mediated by the ANT in maximallypolarized (forward mode of both ANT and ATPase) and maximally depolarized (reverse mode of both ANT ... cyclosporin A was demonstrated in the present study in intact mitochondria. This is the result of an imposing role of the ANT. Apparently, the ADP–ATP exchange rates by the ANT are slower thanthe...

Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx

... Chemistry and Bioengineering, Changsha University of Science and Technology, Hunan, China3 College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan, ... promote the expres-sion of SR-BI, resulting from the binding to putative KLF4 binding element on the promoter of SR-BI. All results indicate a potential function of KLF4 in the pathogenesis of atherosclerosis ... identiﬁed,including CYP1A1, human keratin 4, intestinal alkalinephosphatase, ornithine decarboxylase, histidine decar-boxylase and cyclin D1 [17]. KLF4 regulates the targetgenes by binding to the potential...

Tài liệu Báo cáo khoa học: Comparison of a coq7 deletion mutant with other respiration-defective mutants in ﬁssion yeast doc

... that induction of apt1 and and induc-tion of gpx1 depend solely on the Pap1 and Atf1 tran-scription factors, respectively, and that induction of ctt1 is dependent on both Pap1 and Atf1 in Sc. ... ubiquinone biosynthesis occurs in mitochondria in yeasts. In summary, our results indicate that coq7 is required for ubiquinone biosynthesis and the coq7 mutant is not distinguish-able from other ... the phosphorylation level of the Spc1 MAP kinase. Induction of ctt1, encoding catalase, and apt1, encodinga 25 kDa protein, but not that of gpx1, encoding glutathione peroxidase,was indistinguishable...

Tài liệu Báo cáo khoa học: Inhibition of cobalamin-dependent methionine synthase by substituted benzo-fused heterocycles pptx

... or cofactor. In detail, the N-terminalmodule is the homocysteine (Hcy)-binding domain; the second domain binds 5-methyltetrahydrofolate, the thirddomain binds CH3-Cbl; and the fourth domain ... design of inhibitors of methionine synthase. Our results indicate that the potency of the tested compounds is related to a planar region of the inhibitor that can be positioned in the centre of the ... pathogenesis of anaemias, cancer and neurologicaldisorders. Although the need for potent and speciﬁc inhibitors of methion-ine synthase has been recognized, there is a lack of such agents. In thisstudy,...

Tài liệu Báo cáo khóa học: Inactivation of copper-containing amine oxidases by turnover products doc

... hydrolysis to aldehyde and quinolamine. The band disappeared on admission of oxygen into the solution. The band at 350 nm formed by LCAO, upon bleaching of the radical, suggests that the neutral ... asshown by the measured amounts of H2O2, by the initialintensity of the radical spectrum and by the bleached500 nm band. Which of the two equilibrium species, the radical or the Cu2+-quinolamine, ... cuvette closed by a Teﬂon stopper. The oxygen was consumed by mostsubstrates within the mixing time as revealed by the immediate bleaching of the TPQ 500 nm band and by the appearance of absorption...

Tài liệu Báo cáo khoa học: AcmA of Lactococcus lactis is an N-acetylglucosaminidase with an optimal number of LysM domains for proper functioning ppt

... chainlength of the cells expressing the mutant enzymes, and sedimentation of the cells in standing cultures;(c) their enzymatic activities, both in the cell and supernatant fractions; and (d) autolysis ... proteins, but not in archaeal proteins [14].From an analysis of proteins containing LysMdomains it is clear that the number of domains and their position in the proteins differs greatly [14].Many ... A2, A3 and A4 in the L. lactis NZ9000 mutants acmAD1 and acmAD1 DhtrA, visual-ized by zymographic analysis of culture supernatants of cellsexpressing the AcmA variants. (B) Binding of the AcmA...

Tài liệu Báo cáo khoa học: Enhancement of oxidative stress-induced apoptosis by Hsp105a in mouse embryonal F9 cells pptx

... characterized by swelling of organelles and cells,followed by lysis of the plasma membrane and randomDNA degradation. In contrast, apoptosis is a process that is characterized by cell shrinkage, ... wassuppressed by SB202190, a potent inhibitor of p38, in F9cells. These ﬁndings indicated that the activation of p38 is an essential step for apoptosis in F9 cells and that Hsp105aenhances activation of ... Hsp105a increasetransiently in embryonic tissues during mouse embryogen-esis, and this protein is localized not only in various tissues,but also in apoptotic cells and apoptotic bodies at the interdigital...

Báo cáo khoa học: Activation of activating transcription factor 2 by p38 MAP kinase during apoptosis induced by human amylin in cultured pancreatic b-cells ppt

... enthusiasticassistance with statistical analyses. This work was sup-ported by the Endocore Research Trust, the Maurice and Phyllis Paykel Trust, and the New Zealand Lot-tery Grants Board, and by Programme ... caspase-3activation by 30%; as did combined SB203580 and JNK inhibitor I, by about 70%; and the combination of SB203580, the JNK inhibitor I and acaspase-8 inhibitor, by 100%. These ﬁndings demonstrate the ... activation of the JNK pathway [15,23]. In the current study, we planned to determine whether either of the other two MAP kinases, ERK and p38 kinase, and activation of their target transcription...

Báo cáo khoa học: Inhibition of recombinant human maltase glucoamylase by salacinol and derivatives pdf

... Becausethese inhibitors decrease both hyperglycemia and hyperinsulinemia, they reduce insulin resistance and stress on the beta-cells of the pancreas, thus preventingfurther insulin-dependent disorders ... for use in kinetic and inhibi-tion analysis as well as future use in structural studies.Kinetic analysis conﬁrmed the enzyme activity of the recombinant protein, and inhibition analysis conﬁrmedclassic ... chain of kotalanol.Fig. 2. Schematic diagram of MGA proteinorganization and expression construct.Amino acid boundaries of each of the domains comprising the full size protein, and the region...

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