Báo cáo khoa học: Functional expression of the quinoline 2-oxidoreductase genes (qorMSL) in Pseudomonas putida KT2440 pUF1 and in P putida 86-1 Dqor pUF1 and analysis of the Qor proteins doc

... putida KT2440 pUF1 and P. putida 86-1 D qor pUF1 The apparent K m values of the Qor proteins for quinoline were similar, whereas the apparent k cat value for quinoline of Qor from strain KT2440 pUF1 ... that the pyranopterin part of the cofactor is somehow defective in Qor from strain KT2440 pUF1. UV/Visual spectra of the Qor prote...

Ngày tải lên: 17/03/2014, 10:20

... composi- tion. The temperature acclimation pattern of proportional pooling of subunits with different kinetic and thermodynamic properties of the tetrameric enzyme may result in fine-tuning of the ... area; this is expected to manifest in the kinetic and thermodynamic properties of the assembled tetramer. In warm-acclimated fish the relative expression between...

Ngày tải lên: 19/02/2014, 02:20

... quantitation of microgram quantities of protein uti- lizing the principle of protein-dye binding. Anal Bio- chem 72, 248–254. 48 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the ... 895–896. Supporting information The following supplementary material is available: Fig. S1. Disintegration of the coupled spin system by the elimination of...

Ngày tải lên: 15/02/2014, 01:20

... per well) for expression of the GAL4 DNA- binding domain ( DNA-BD) f usio n p rotein , together with the l uciferase repo rter plasmid pG5luc carrying five GAL4-binding sites up- stream of the TAT A ... mutant proteins (including VP16-fused protein) and luciferase protein, as a control product, were synthesized by using the TNT Quick Coupled Transcription/Translation S yste...

Ngày tải lên: 19/02/2014, 16:20

... the N-terminal helices 1 and 2 while leaving the interacting a-helix 8 intact, may destabilize the proteins leading to spurious protein–protein or /and protein–DNA interactions. The crystal structure of ... prevails in the presence of divalent cations [24], into an unfolded square-planar configuration [14,20]. Binding of CCE1 results in the complete unpairing of t...

Ngày tải lên: 20/02/2014, 11:20

... Pex1 5p- TEV-ProtA Pex1 5p- TEV-ProtA Pex1 1p Pex1 0p Pex1 7p Pex1 3p Pex1 4p Pex 5p Pex 6p Pex 1p detergent extract - Pex 6p- TEV-Pro A tA Pex 6p- TEV-ProtA Pex1 1p Pex1 0p Pex1 7p Pex1 3p Pex1 4p Pex 5p Pex1 5p Pex 1p * detergent ... Pex 6p- TEV-Pro A tA Pex 6p- TEV-ProtA Pex1 1p Pex1 0p Pex1 7p Pex1 3p Pex1 4p Pex 5p Pex1 5p Pex 1p * detergent extract - Pex 6p- TEV-...

Ngày tải lên: 07/03/2014, 12:20

... in overexpression of GAL4, in turn inducing an increase of the expression of the OR gene under control of GAL1. The pJH2 vector contains the URA3-selectable marker. Two G a protein expression ... replacing the hph coding sequence from the pRHF-hph plasmid [35] by the luciferase coding sequence. In this vector the Photinus pyralis cDNA sequence is placed downs...

Ngày tải lên: 07/03/2014, 16:20

... (Sigma). Electrophoresis and in- gel activity staining Wild-type and mutant proteins were separated from other proteins in their respective preparations by native PAGE using 10% (w ⁄ v) gels [33]. They were then subjected ... calculated using origin pro 7.5 software (OriginLab Corp., Northampton, MA, USA). The concentrations of the proteins in the preparations were dete...

Ngày tải lên: 16/03/2014, 04:20

... micro-organism. Characterization of wild-type Pf PDO A detailed study of Pf PDO structure highlighted certain putative ATP-binding sites (the presence of P- loops, a common m otif in ATP-binding proteins) , t he primary structure ... K d forATPwasobtainedusingtheprogram KALEIDA GRAPH 3.0. Fig. 5. ATP-binding capacity of PfPDO. Cross-linking of PfPDO w ith 8-azido-[ 32 P] ATP[a...

Ngày tải lên: 16/03/2014, 16:20

... estimated for the interaction between spinach Pc and fern PSI. The k obs values with fern Pc and those for the slower phase with spinach Pc exhibit saturation profiles at increasing donor protein concentra- tions ... at pH 5, a value close to the physiological pH. As shown in Table 1, lowering pH only has quantitatively minor effects i n the interaction between fernPSIandspinac...

Ngày tải lên: 16/03/2014, 16:20