... wasperformed using LipofectAMINETM(Life TechnologiesInc.) according to the manufacturer’s protocol. 293T cellswere maintained in minimum Eagle’s medium (MEM)containing 10% (v/v) fetal bovine serum ... either kinase-dead TrkAreceptors or by the inhibitory Smad7 proteinTo assess the involvement of TrkA receptors in the activation of the Smad pathway, different TrkA variantswere tested in ... tissues, therebycausing distinct cell-specific effects depending on the presentcomposition of receptors, Smad proteins and DNA-bindingpartners [2,3]. Referring to cell populations of neuronalorigin,...