... required, were incuba- ted for 1 min at 37 °C in a Dubnoff water bath under gentle shaking in the presence of 1 lCiÆmL )1 L -[2,3,4- 3 H]arginine, unlabelled L -arginine and L -leucine when required ... small inhibition of L -arginine in ux by L -leucine in the absence of Na + could be due probably to the presence of the system b + [16] but this component accounts for 5–7% o...
Ngày tải lên: 08/03/2014, 02:20
... subheading and then select the Proteomics link). One of the proteins of interest to the Cell Migration Consortium is paxillin, a scaffolding protein involved in focal adhesions [9]. Shown in Fig. ... building blocks of eukaryotic chromatin [15]. A host of PTMs reside on the N-terminal tails of the histones, and this array of PTMs has been well documented and includes mon...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching doc
... dynamics and kinetic properties of proteins in living cells [12–19]. FCS detects the fluctuations of fluorescent intensity derived from the movement of a single fluorescent molecule in a very tiny observation ... proper- ties of proteins [13] and binding interactions [14]. FRAP is a conventional technique used to study the kinetic properties of proteins in a cell by measuring...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Analysis of oxidative events induced by expanded polyglutamine huntingtin exon 1 that are differentially restored by expression of heat shock proteins or treatment with an antioxidant ppt
... ROS and with increased formation of carbonyl residues in proteins. The enhanced oxidation of pro- teins observed in httEx1-polyQ-expressing cells was not found to correlate with an increased intracellular level ... heat-inactivated FBS. The medium included penicillin and streptomycin (10 4 UÆL )1 ) (Gibco, Invitrogen), and 250 lgÆmL )1 Fungizone (Gibco, Invitro- gen). The cells...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khoa học: "ANALYSIS OF OONOUNCTIONS IN PAKSER" potx
... new pieces of information, which were implicit in the "linear" input form, are made explicit in the result of the analysis. These pieces of information are extracted using the syn- ... fol- lowing it 3) The conj~tion is inserted in the node the first I~ that is found going up in the hierarchy (in fig.2.c, starting from C~NN2 and going u~s, we find 1:m.&a...
Ngày tải lên: 21/02/2014, 20:20
Báo cáo khoa học: Analysis of DNA-binding sites on Mhr1, a yeast mitochondrial ATP-independent homologous pairing protein potx
... DNA-binding activities were not completely lost. We also examined the DNA-binding activity of Mhr1 in the absence of Mg 2+ , because the DNA- binding activities of other HP proteins are often increased ... assessed by comparing the amounts of DNA remaining at the original positions (ssDNA and dsDNA are indicated by filled and open arrowheads in Fig. 5) and the amounts of DN...
Ngày tải lên: 06/03/2014, 09:22
Báo cáo khoa học: Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast ppt
... accumulates in cyto- plasmic foci in 5–20% of cells under stress condi- tions (Fig. 5B), suggesting that the N-terminal domain of Lsm1p is sufficient in combination with the Sm and C-terminal domains of ... C-terminal domain deleted is consistent with this (Figs 4 and 5). Hybrid proteins carrying the N-terminus of one protein and the Sm domain of the other localize accord...
Ngày tải lên: 07/03/2014, 02:20
Báo cáo khoa học: Analysis of the NADH-dependent retinaldehyde reductase activity of amphioxus retinol dehydrogenase enzymes enhances our understanding of the evolution of the retinol dehydrogenase family pot
... pmol and 99 pmol of retinol per mg of total protein after 1 h of incubation with 10 lm and 20 lm of retinal, respectively. RDH2 enzymes were more effi- cient than RDH1 enzymes, and generated 64 and 134 ... active site (YTVAK) and the cofactor-binding motifs are marked in bold. The D43, T67 and A69 residues, involved in cofactor specificity, are indicated by asterisks. Fl...
Ngày tải lên: 07/03/2014, 09:20
Báo cáo khoa học: "Analysis of Unknown Words through Morphological Decomposition" potx
... make use of sets of characters. Anything is a set consisting of all surface char- acters, BCDFGKPSTW and HJLMNqRVXYZ are sets consisting of those letters, V is the set of vowels and C the ... ending in s could be either a plural noun or a third person singular verb. These points can multiply together and of- ten produce a large number of possible analyses. Out o...
Ngày tải lên: 09/03/2014, 01:20
Báo cáo khoa học: Analysis of the regulatory motifs in eukaryotic initiation factor 4E-binding protein 1 pot
... & Proud CG (2005) Distinct signaling events downstream of mTOR cooperate to mediate the effects of amino acids and insulin on initiation factor 4E-binding proteins. Mol Cell Biol 25, 2558–2572. 30 ... interaction of 4E-BP1 with raptor and the amino acid residues required for functional RAIP and TOS motifs, as assessed by raptor binding and the phosphoryla- tion of 4E-BP1...
Ngày tải lên: 16/03/2014, 06:20