Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx
Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx
... Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. Ram Savan 1 , Daisuke Igawa 2 and Masahiro Sakai 2 1 United Graduate School of Agricultural ... GAGGCTAGATACTGCTCGATGT IL-10 forward3 TGATGATTTGGAACCATTATTGAA IL-10 reverse3 CACCTTTTTCCTTCATCTTTTCAT b-Actin forward1 ACTACCTCATGAAGATCCTG b-Actin reverse1 TTGCTGATCCAC...
Ngày tải lên: 20/02/2014, 02:21
Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt
... is cleaved enzymatically, and the product can be measured photometrically. This was carried out on an ELISA reader (Rainbow, Tecan, Crailsham, Germany) at a wavelength of 450 nm, and the values ... temperature until all free water was evaporated. After this, IR spectra were recorded at room temperature and at 37 °C. Usually, the original spectra were evaluated directly and...
Ngày tải lên: 21/02/2014, 00:20
Báo cáo khoa học: Identification and expression analysis of an IL-18 homologue and its alternatively spliced form in rainbow trout (Oncorhynchus mykiss) doc
... CGACATTTCCGAGTGACGTTC 3¢-RACE R2 CCTTCAACACCCTGACTTCAC 5¢-RACE R3 ATGTCCTCCTTGTCTACTACC 5¢-RACE EF1 AGCAGCTCCGAATGTAAGGTG IL-1 8A expression EF2 GCTCCGAATTCGAACATGAC IL-18B expression ER1 AGGCAAAGGTTGCTCCAGTG ... AGGCAAAGGTTGCTCCAGTG IL-18 expression Actin-F ATGGAAGATGAAATCGCC Gene expression Actin-R TGCCAGATCTTCTCCATG Gene expression RR1 TGGTACCACTCAACATGTCAGTAAGCG Gene orga...
Ngày tải lên: 16/03/2014, 16:20
Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx
... enzymatic characterization of HpSDH demonstrates its activity with k cat of 7.7 s )1 and K m of 0.148 mm toward shikimate, k cat of 7.1 s )1 and K m of 0.182 mm toward NADP, k cat of 5.2 s )1 and ... the shikimate path- way, which is essential for the synthesis of important metabolites, such as aromatic amino acids, folic acid, and ubiquinone [10]. The shikimate...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf
... some sera to a 9- and a 15-kDa band. We could show that the 9-kDa band in the tomato extracts reacts with a specific antibody against the LTP from cherry, Pru av 3 and the 15-kDa band shows reactivity ... 5¢TAT GCGTGGTCCAATGCTATGC, and FF 3A, matching with the C-terminal sequence of the coding region: 5¢TTAC AAGGACAAATTAATTGTGCCAG. For amplification of the long i...
Ngày tải lên: 21/02/2014, 00:20
Báo cáo khoa học: Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal b-N-acetylhexosaminidase pot
... more than 0.3 nm. Most (83.5%) of the amino acid residues are plotted in the favourable regions of the Ramachandran plot. The deviation of geometrical parameters from ideal values (G-factors) ... hydrolysis of N-formyl, N-glycolyl and N-propionyl derivatives of the standard N-acetyl substrate) than A. oryzae (2–5% hydrolysis). The cloning and sequencing of PoHex...
Ngày tải lên: 14/03/2014, 23:20
Tài liệu Báo cáo khoa học: Complex transcriptional and translational regulation of iPLA2c resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization docx
... TGGAAAGCTTGCCACATCAGTCTACAAAG 85R TGCTCCATGGTGGCATCCCAATATGTAAACCA 83 83F GAACCAAGCTTGAAGCACATTCTTGCAGTAAGCA 83R CAAAACATGTTGGCTACGGGACATACAAATGTTCA 80 80F GTTGAAGCTTTTTGAAACTTAGCACTTCTGC 80R ATTCCATGGTGGCTGAAATCATTTCATTTTGATTGCC 74 ... synthesized and annealed: g50 (5¢-TATTAATCTGACTGTAGATATAT ATATATTACCTCCTTAGTAATGC-3¢) and random- ized control g50c (5¢-TTGATAGTTATCTATTACAG TCTTCTTAGATTG...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Purification and cDNA cloning of a cellulase from abalone Haliotis discus hannai ppt
... CTGATCTAGAGGTACCGGATCC 5RACF ATCCTCACGAACAAGCAG 5RACR GATCGCGATGCAGGCCTT FLF1 GGACGACTACAGCGTCTTCAGTAGA FLR1 TCCAAACAGTCAGTTTCTTAACCGT Table 1. Purification of cellulase from abalone Haliotis discus hannai. ... of the cDNA library and cloning of cellulase cDNA was achieved as follows: Total RNA was extracted from 1 g of abalone hepatopancreas by the ganidinium thiocyanate-phe...
Ngày tải lên: 20/02/2014, 23:20
Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf
... chitinases have catalytic domains that are at least as large as those of the plant enzymes and that may contain at least six subsites [26,30]. However, the catalytic domains of ChiG, ChiC and most other ... 1084–1092. 35 Kawase T, Yokokawa S, Saito A, Fujii T, Nikaidou N, Miyashita K & Watanabe T (2006) Comparison of enzymatic and antifungal properties between family 18...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... 276 93QEGYGSTQLPSDEPLNGLND CAA GAA GGA TAT GGC AGC ACC CAA CTT CCA TCG GAC GAG CCA TTG AAC GGG CTC AAC GAC 336 113KAIQDILNEHNMFRAKERVP AAG GCC ATA CAG GAC ATC CTC AAC GAG CAC AAC ATG TTC CGC GCC AAG GAG CGC GTC CCG ... GAG CAG GTA GCG TCC GAG TCA GCC TCC CTC ACA ACA CCA 216 73TTREEQPAAEAAASTAGGSQ ACC ACC AGG GAA GAA CAA CCG GCG GCA GAG GCC GCG GCG TCC ACC GCT GGC GGT AGC CAA 276 93QEGYGSTQLPS...
Ngày tải lên: 07/03/2014, 12:20