Báo cáo khoa học: Glucagon-like peptide-2 stimulates the proliferation of cultured rat astrocytes pptx

... Glucagon-like peptide-2 stimulates the proliferation of cultured rat astrocytes Esther Vela ´ zquez, Juan M. Ruiz-Albusac and Enrique Bla ´ zquez Department of Biochemistry and ... determine whether or not GLP-2 has any effect on cellular proliferation on neural cells, we examined the effects of this peptide on cultured astrocytes from rat cerebral cortex. The...

Ngày tải lên: 17/03/2014, 03:20

... containing the mutations of the miR-23a binding site (Fig. 4A). It was shown that miR-23a ASO or pcDNA3 ⁄ pri-23a did not affect the intensity of EGFP fluorescence in the vector bearing the mutant of the ... confirmed the active role of miR-23a in the growth promotion of malignant cells. The fundamental function of miRNAs is to regulate their targets by direct clea...

Ngày tải lên: 18/02/2014, 04:20

... models of AD research to increase the degree of phosphorylation of various proteins, such as tau. One of the hallmarks of AD and tauopathies is the appearance of highly phos- phorylated tau isoforms ... OA caused the loss of stabilization of focal adhesions and a consequent loss of cytoskeletal organization in keratinocytes, due to an alteration in the tyrosine-pho...

Ngày tải lên: 18/02/2014, 14:20

... observations, the rate of cofilin binding to F-actin in the presence of 2–30 mm Pi is the same at pH 6.5 and 8.0. The influ- ence of Pi on the relative rates of cofilin binding at these two pH values ... pertinent to the regulation of actin dynamics in the cell. Results Fluorescence measurements of the effect of Pi on the rate of cofilin binding to TRC–F-actin A...

Ngày tải lên: 19/02/2014, 07:20

... may enter the citric acid cycle completing the catabolism of the pyrrolidine moiety of nicotine. Expression of the genes of these enzymes was dependent on the presence of nicotine in the growth ... expression of these genes depends on the presence of nicotine in the growth medium and we have determined the enzyme activities of the proteins. Our results demo...

Ngày tải lên: 19/02/2014, 07:20

... mutant strain. However, the amounts of core protein 1 and core protein 2 were relatively unaffected. Therefore, the absence of subunit 6 appeared to alter the rates of processing of two of the redox subunits ... antibodies to the subunits of the yeast bc 1 complex indicated on the left side of the blots. The Western blots are shown in panels A and B and the r...

Ngày tải lên: 19/02/2014, 12:20

... with the familiar pattern of expression of the principally embryonic cA–F genes in the rodent lens [41,47]. The absence of expression in outer layers of the lens contrasts with the expression of ... polypeptide capable of completing the C-terminal domain of the protein. Currently there is no evidence for expression of canonical cN in primates. This leaves open...

Ngày tải lên: 19/02/2014, 17:20

... the question arises concerning the mechanisms underlying the orchestrated expression of these enzymes by the oxygen tension. The findings that the effect of hypoxia on the gene expression of the ... clear twofold increase of PDI mRNA, a more moderate increase of the mRNAs for prolyl hydroxylases, and did not increase the mRNA abundance of the other enzymes. Di...

Ngày tải lên: 20/02/2014, 02:21

... also observed in the rate of degradation of the remaining mutant protein (20% left of the protein) vs. the wild type (40% left of the protein) at 3 h of chase (Fig. 7A,B). We also analyzed the rate of degradation of ... Ga 16 and Ga q subunits. Therefore the lack of change in the rate of degradation cannot be due to lack of receptor-activa- tion of the...

Ngày tải lên: 20/02/2014, 03:20

... important aspect of this work was the assessment of the effect of novel Fe chelators on the uptake and fate of NTBI and to investigate the potential of these chelators for therapeutic use in Fe overload ... and proliferation, is uncertain. Hence, it is of interest to investigate the uptake and metabolism of NTBI in normal and cancer cells, and the effect of Fe che...

Ngày tải lên: 20/02/2014, 11:20