Báo cáo khoa học: The molecular chaperone a-crystallin incorporated into red cell ghosts protects membrane Na/K-ATPase against glycation and oxidative stress ppt

... The molecular chaperone a-crystallin incorporated into red cell ghosts protects membrane Na/K-ATPase against glycation and oxidative stress Barry K. Derham 1 , J. ... system in which a-crystallin was incorporated into red cell ghosts . Its ability to protect the intrinsic membrane protein Na/K-ATPase from external stresses was studied. Red...

Ngày tải lên: 17/03/2014, 03:20

... covered scissile peptide bonds appear at the molecular surface and become exposed to the solvent; the exposed peptide bonds are digested by one another and the enzyme becomes inactive. We hypothesize ... changes on the surface. On the other hand, anionic surfactants such as SDS and LAS bind to the surface regions of protein, and the surface regions would be unfolded...

Ngày tải lên: 21/02/2014, 03:20

... incubat- ing the cultured cells in IFG-free medium for 24 h prior to direct assay of the cell lysates. IFG incubation also increased the lysosomal trafficking of L444P GCase in fibroblasts and reduced the ... GCase mutations. The data for each cell line have been normalized to the GCase activity in untreated cells, and are presented as the mean ± SEM. F, fibroblast; L, lymp...

Ngày tải lên: 22/03/2014, 21:20

... in the intermembrane space binds Ca 2+ and associates spontaneously with the inner membrane; sec- ond, it carries Ca 2+ through the membrane and some- how returns back to the outer surface of the ... by cyclophilin D or other chaperones but Ca 2+ accumula- tion and or oxidative stress increase the number of misfolded proteins. When the number of protein clus- ters e...

Ngày tải lên: 23/03/2014, 03:20

... started by the addition of the 10 lm hemin unless otherwise stated. Spinach (Spinacia oleracea) ferredoxin and spinach ferredoxin NADP + reductase were obtained from the Sig- ma-Aldrich Company Ltd. The ... [14] and the aurea and yellow-green-2 (yg-2) mutants of tomato [15]. Typically, these mutants have elongated stems or hypocotyls, reduced red and far -red responses du...

Ngày tải lên: 30/03/2014, 11:20

... two-domain organization and the presence of both a-helices and b-strands. On the basis of these data and the fact that alr2505-expression is specifically induced under oxidative stress, we nemed this ... to apoferredoxin, and the finding that slr0387 and sll0704 mutants were obtained suggested that none of them was essential for the viability of this cyanobacterium [24]....

Ngày tải lên: 18/02/2014, 04:20

... confor- mation with the modified dG residue and the partner base dC in the opposite strand displaced into the minor and major grooves, respectively [9]. Molecular views of these (+)-cis and (+)-trans ... contain- ing the target recognition domain (Fig. 5B). The DNA molecule is located in the cleft formed between the two domains with the major groove facing the sma...

Ngày tải lên: 19/02/2014, 00:20

... of the ER–Golgi vesicle transport. Through the use of mark- ers for the ER (PDI) and the Golgi apparatus (GM130, p230), the phosphorylated isoforms of the K650M mutant were identified in both the ... receptors were pro- perly processed and expressed at the cell surface. Whe- ther disulfide bonding between two mutant receptors occurred intracellularly or at the plasma me...

Ngày tải lên: 19/02/2014, 00:20

... sensor protein KdpD inserts into the Escherichia coli membrane independent of the Sec translocase and YidC Sandra J. Facey and Andreas Kuhn Institute of Microbiology and Molecular Biology, University ... is inserted into the membrane in the absence of SecA, SecE and YidC. Short epitopes introduced into the periplasmic regions allow the analysis of insertion eve...

Ngày tải lên: 20/02/2014, 11:20

... accumu- lates in the nucleus during the G2 phase of the cell cycle and promotes cell division. J Cell Biol 181, 485–496. 27 Kim J & Roeder RG (2009) Direct Bre1-Paf1 complex interactions and RING ... ubiquitinated through a Rac- and Unkempt-dependent process, the molecular composi- tion of the E3 ligase involved and the role of Unkempt RING finger remain uncerta...

Ngày tải lên: 06/03/2014, 09:22