Báo cáo khoa học: Purple membrane lipid control of bacteriorhodopsin conformational ﬂexibility and photocycle activity An infrared spectroscopic study docx

... theeffects of purple membrane lipids on both the M turnovertime constants and the ﬂexibility of the bacteriorhodopsin transmembrane a-helices.Materials and methods Purple membranes were extracted ... Purple membrane lipid control of bacteriorhodopsin conformational ﬂexibility and photocycle activity An infrared spectroscopic study Richard W. Hendler1, Steven M. Barnett2, Swetlana ... native purple membrane (solidline), purple membrane exposed brieﬂy toTriton (dashed line), and purple membrane reconstituted with purple membrane lipids insolutions without NaCl (dotted line) and...

Tài liệu Báo cáo khoa học: Fatty acid synthesis Role of active site histidines and lysine in Cys-His-His-type b-ketoacyl-acyl carrier protein synthases ppt

... position 328 is an importantfactor for efﬁcient transacylation activity, especially at4 °C.Elongation activity of the wild-type and K328mutant KAS I proteinsThe transfer and decarboxylation ... NovoNordisk Foundation and the Danish Natural ScienceResearch Council through DANSYNC and grant21-01-0622. Dr Andy Thompson (ID29, ESRF), DrSean McSweeney (ID14.4 also ESRF) and Dr YngveCerenius ... acknowledged for theirassistance with data collection. We thank AnnetteKure Andreassen, Marianne Mortensen and AugustaPalsdottir for outstanding technical assistance.References1 Huang W, Jia J, Edwards...

Tài liệu Báo cáo khóa học: Non-speciﬁc depolymerization of chitosan by pronase and characterization of the resultant products pptx

... comparison of band absorbance. Absence of a sharp and convolutedspectral band around 3600–3000 cm)1in the spectra of bothchitosan and LMWC indicated the absence of free -OHgroups and the involvement ... magic angle at6.2 kHz for native chitosan and 7 kHz for LMWC and chito-oligomers + monomer. A contact time of 1 ms and apulse repetition time of 5 ms were used, and more than 2000scans were ... the biofunctionalities of these materials.AcknowledgementsAuthors thank Dr S. Subramanian (Indian Institute of Science,Bangalore) for X-ray analysis, Dr A.G. Appu Rao and Dr SrideviAnnapurna...

Báo cáo khoa học: Tumor suppressor p16INK4a ) modulator of glycomic proﬁle and galectin-1 expression to increase susceptibility to carbohydrate-dependent induction of anoikis in pancreatic carcinoma cells ppt

... globo- and isoglobotetraosyl-ceramides and not mucin-type O-glycans. An apparentFig. 1. Quantitative proﬁling of N-glycans. Complete representation of the N-glycan proﬁles of mock- and p16INK4a-transfected ... plantlectins (for an explanation of the acronyms and listing of oligosaccharide speciﬁcity, please see Table 2) of mock-transfected (gray line) and p16INK4a-transfected (black line) Capan-1 pancreatic ... Dawson SA (2004) Transfec-tion of 2,6 and 2,3-sialyltransferase genes and GlcNAc-transferase genes into human glioma cell line U-373MG affects glycoconjugate expression and enhancescell death....

Báo cáo khóa học: Endoplasmic reticulum-associated degradation of glycoproteins bearing Man5GlcNAc2 and Man9GlcNAc2 species in the MI8-5 CHO cell line docx

... degradation of glycoproteinsbearing Man5GlcNAc2 and Man9GlcNAc2 species in the MI8-5CHO cell lineFranc¸ois Foulquier, Sandrine Duvet, Andre´Klein, Anne-Marie Mir, Fre´de´ric Chirat and Rene´CacanUnite´de ... concen-tration, various proportions of Man5GlcNAc2 and Man9GlcNAc2 species could be transferred to proteins.To obtain an equal distribution of the radioactivity betweenthe Man5 and Man9 populations bound ... decreasingamounts of Man9GlcNAc2 and Man5GlcNAc2 correlatedwith the increasing amounts of Glc1Man9GlcNAc2 and peak X, respectively. Furthermore, as expected, peak X and Glc1Man9GlcNAc2 were completely...

Tài liệu Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane pdf

... dimerization of the bc1complex atthis stage (i.e. just after the addition of ISP and Qcr10p).On the other hand, in the transition from the 500 kDaband to the 670 kDa band, a structural rearrangement of ... (°C)00ABFig. 7. Stability of the 500 kDa bc1sub-complex in different condi-tions of solubilization. (A) Mitochondrial membranes from wild-type(WT) (lanes 1 and 2) and DQCR9 (lanes 3 and 4) yeast strains ... Analy-sis of molecular masses and oligomeric states of proteincomplex by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional nativeelectrophoresis. Anal...

Tài liệu Báo cáo khoa học: Oocyte membrane localization of vitellogenin receptor coincides with queen ﬂying age, and receptor silencing by RNAi disrupts egg formation in ﬁre ant virgin queens ppt

... pairs of ovaries) and mated queens (lanes 2 and 4; protein from four pairs of ovaries) was analyzed by westernblot (primary antibody: anti-SiVgR sera in lanes 1 and 2 and preim-mune serum in lanes ... receptor (Si VgR). Membrane proteins (10 lg) from ovary (lane 1), head (lane 2), fatbody (lane 3) and gut (lane 4) of mated queens, and from abdomen of adult males (lane 5) were analyzed by western ... and 4; both 1 : 1000 dilution). A band of  202 kDa was recognized by the Si VgR antisera in ovaries fromvirgin (lane 1) and mated queens (lane 2, arrow). The relative VgRband intensity (lane...

Tài liệu Báo cáo khoa học: Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae docx

... have an unusual primarystructure with many strands of alternating hydrophi-lic and hydrophobic residues and a high abundance of aromatic residues that tend to be placed at thestart of the strands ... integral membrane proteins fromthe mitochondrial outer membrane, and of the 11 mostabundant integral proteins, 10 had a-helical transmem-brane segments. Of these 10 proteins, most had asingle transmembrane ... trans- membrane segments that are assembled into the mito-chondrial outer membrane. Results and DiscussionIntegral membrane proteins in the mitochondrialouter membrane The protein proﬁle of mitochondrial...

Báo cáo khoa học: How a lipid mediates tumour suppression Delivered on 29 June 2010 at the 35th FEBS Congress in Gothenburg, Sweden pdf

... intracellular membrane systems, and many vital cellular processes,such as metabolic reactions, signal transduction, cyto-skeletal rearrangements, protein sorting and regulation of membrane dynamics, ... mitosis, and that this causes its dissociationfrom Beclin 1 and an inhibition of autophagy [18].Recognition of PtdIns3P by FYVE and phox homology (PX) domainsA breakthrough in our understanding of ... phagophore membrane is still a matter of debate, although thereare strong arguments in favour of a contribution fromboth ER and mitochondrial membranes [42,64,65]. Atleast in the case of ER membranes,...

Báo cáo khoa học: Differential membrane compartmentalization of Ret by PTB-adaptor engagement pdf

... expressing Retmutants were analyzed in transwell chemotaxis assays after 14 h,with Ret ligands supplied below the membrane and CO suppliedabove and below the membrane. Two-wayANOVA compared ... the presence of Frs2 or Shc. To conﬁrm therelative purity of raft and nonraft membranes, DRM and SUP fractions were immunoblotted towards the lipid raft marker ﬂotillin-1 and the transferrin recep-tor ... GJ (2007) A glance at the struc-tural and functional diversity of membrane lipids.Methods Mol Biol 400, 1–13.16 Brown DA (2006) Lipid rafts, detergent-resistant mem-branes, and raft targeting...

Xem thêm