Báo cáo khoa học: Purple membrane lipid control of bacteriorhodopsin conformational flexibility and photocycle activity An infrared spectroscopic study docx

... the effects of purple membrane lipids on both the M turnover time constants and the flexibility of the bacteriorhodopsin transmembrane a-helices. Materials and methods Purple membranes were extracted ... Purple membrane lipid control of bacteriorhodopsin conformational flexibility and photocycle activity An infrared spectroscopic study Richard...

Ngày tải lên: 17/03/2014, 03:20

... position 328 is an important factor for efficient transacylation activity, especially at 4 °C. Elongation activity of the wild-type and K328 mutant KAS I proteins The transfer and decarboxylation ... Novo Nordisk Foundation and the Danish Natural Science Research Council through DANSYNC and grant 21-01-0622. Dr Andy Thompson (ID29, ESRF), Dr Sean McSweeney (ID14.4 also ESRF) an...

Ngày tải lên: 19/02/2014, 08:20

... comparison of band absorbance. Absence of a sharp and convoluted spectral band around 3600–3000 cm )1 in the spectra of both chitosan and LMWC indicated the absence of free -OH groups and the involvement ... magic angle at 6.2 kHz for native chitosan and 7 kHz for LMWC and chito-oligomers + monomer. A contact time of 1 ms and a pulse repetition time of 5 ms were used,...

Ngày tải lên: 19/02/2014, 12:20

... globo- and isoglobotetraosyl- ceramides and not mucin-type O-glycans. An apparent Fig. 1. Quantitative profiling of N-glycans. Complete representation of the N-glycan profiles of mock- and p16 INK4a -transfected ... plant lectins (for an explanation of the acronyms and listing of oligosaccharide specificity, please see Table 2) of mock-transfected (gray line) and p16 IN...

Ngày tải lên: 16/03/2014, 10:20

... degradation of glycoproteins bearing Man5GlcNAc2 and Man9GlcNAc2 species in the MI8-5 CHO cell line Franc¸ois Foulquier, Sandrine Duvet, Andre ´ Klein, Anne-Marie Mir, Fre ´ de ´ ric Chirat and Rene ´ Cacan Unite ´ de ... concen- tration, various proportions of Man5GlcNAc2 and Man9GlcNAc2 species could be transferred to proteins. To obtain an equal distribution of the radioactiv...

Ngày tải lên: 16/03/2014, 16:20

... dimerization of the bc 1 complex at this stage (i.e. just after the addition of ISP and Qcr10p). On the other hand, in the transition from the 500 kDa band to the 670 kDa band, a structural rearrangement of ... (°C) 0 0 A B Fig. 7. Stability of the 500 kDa bc 1 sub-complex in different condi- tions of solubilization. (A) Mitochondrial membranes from wild-type (WT) (lanes 1 and 2...

Ngày tải lên: 18/02/2014, 08:20

... pairs of ovaries) and mated queens (lanes 2 and 4; protein from four pairs of ovaries) was analyzed by western blot (primary antibody: anti-SiVgR sera in lanes 1 and 2 and preim- mune serum in lanes ... receptor (Si VgR). Membrane proteins (10 lg) from ovary (lane 1), head (lane 2), fat body (lane 3) and gut (lane 4) of mated queens, and from abdomen of adult males (lane...

Ngày tải lên: 18/02/2014, 08:20

... have an unusual primary structure with many strands of alternating hydrophi- lic and hydrophobic residues and a high abundance of aromatic residues that tend to be placed at the start of the strands ... integral membrane proteins from the mitochondrial outer membrane, and of the 11 most abundant integral proteins, 10 had a-helical transmem- brane segments. Of these 10 pro...

Ngày tải lên: 19/02/2014, 07:20

... intracellular membrane systems, and many vital cellular processes, such as metabolic reactions, signal transduction, cyto- skeletal rearrangements, protein sorting and regulation of membrane dynamics, ... mitosis, and that this causes its dissociation from Beclin 1 and an inhibition of autophagy [18]. Recognition of PtdIns3P by FYVE and phox homology (PX) domains A break...

Ngày tải lên: 06/03/2014, 01:23

... expressing Ret mutants were analyzed in transwell chemotaxis assays after 14 h, with Ret ligands supplied below the membrane and CO supplied above and below the membrane. Two-way ANOVA compared ... the presence of Frs2 or Shc. To confirm the relative purity of raft and nonraft membranes, DRM and SUP fractions were immunoblotted towards the lipid raft marker flotillin-1 and th...

Ngày tải lên: 07/03/2014, 05:20