Báo cáo khoa học: Biochemical characterization of human umbilical vein endothelial cell membrane bound acetylcholinesterase ppt

... are endothelial cells from the vein of human umbilical cord. Flow cytometry of endothelial cells The results of flow cytometry (Fig. 2) revealed that the HUVECs showed constitutive expression of ... and several of the compo- nents of the neuronal cholinergic system are not only Keywords acetylcholinesterase; biochemical characterization; cellular membrane; human e...

Ngày tải lên: 16/03/2014, 23:20

... 2006 FEBS Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism Matthias Mack 1 , Ute Schniegler-Mattox 2 , Verena Peters 3 , Georg F. Hoffmann 3 , ... group of the CoA-acceptor (R 2 -COO – ). In the case of glutaconate CoA-transferase from A. fermentans, CoAS – transiently is bound to the c-carboxyl group of bE54 [24]. M. Ma...

Ngày tải lên: 19/02/2014, 07:20

... of the enzyme for that cofactor and the apparent absence of a redox process as part of the catalytic cycle. The substrate binding site of the type II enzyme remains veiled. However, a patch of ... influence of substrates and cofactors on the quaternary structure of the enzyme, we performed boundary sedimentation experiments under different experi- mental conditions. In the absenc...

Ngày tải lên: 19/02/2014, 13:20

... specificity and enzymatic properties of USP7 As part of the characterization of USP7 biochemical properties, we have measured its kinetic parameters for the hydrolysis of ubiquitin C-terminal 7-amido-4-meth- ylcoumarin ... molar ratio of 100 : 1 in a final volume of 150 lL. Reactions were stopped by addition of one volume of 2 · Laemmli sample buffer, followed by boiling at...

Ngày tải lên: 07/03/2014, 05:20

... reduction of flavin [13,14], and the transfer of electrons to ubiqui- none, which is part of the respiratory chain. Because of this connection, pyrimidine formation requires a sufficient concentration of ... identified as a transmembrane domain of 17 amino acids in rat DHODH [12], can be presumed to be a membrane- spanning a-helix. Here, we were able to predict a transmembrane domai...

Ngày tải lên: 07/03/2014, 12:20

... the surface of endothelial cells [4], annex- in A1 has been observed to bind to an extracellular domain of b 2 integrin [5]. Annexin A5 was found to bind to the intracellular part of the b 5 integrin ... Winter et al. Biochemical characterization of annexin B1 FEBS Journal 273 (2006) 3238–3247 ª 2006 The Authors Journal compilation ª 2006 FEBS 3247 Biochemical characterizatio...

Ngày tải lên: 07/03/2014, 12:20

... Authors Journal compilation ª 2007 FEBS 1201 Biochemical characterization of rice trehalose-6-phosphate phosphatases supports distinctive functions of these plant enzymes Shuhei Shima 1,2 , Hirokazu ... function of trehalose biosynthesis in the regulation of starch biosynthesis has recently been revealed. Trehalose feeding was found to induce expression of ApL3, encoding a large...

Ngày tải lên: 16/03/2014, 11:20

... content of approximately 5.4% (Table 2). From the overall sugar content (HPAEC) and the estimated sugar contents of the indi- vidual glycoforms (MALDI-TOF-MS), the proportions of glycoforms I ... properties of SPC4. The absorption spectrum of purified SPC4 was recorded in 50 m M sodium acetate pH 5. The inset shows the spectrum of the extract- ed heme. (B) MALDI-TOF-MS analysis of...

Ngày tải lên: 16/03/2014, 13:20

... incorporation of GMP residues into the opposite 3¢-end of the mutant RNA. A second mutation of the U6 substrate RNA consisted of the introduction of two additional AMP nucleotides in front of the oligo(A) ... length of the UMP tails of the substrate RNA, a decreasing number of distinguishable labeled RNA products was obtained: three bands in lane 2, two in Fig. 1. Ionic requ...

Ngày tải lên: 17/03/2014, 09:20

... in a 15-fold increase in the yield of the soluble protein. Pantetheinylation of the ACP in ACP- CON didomain was verified using MALDI-TOF of a tryptic fragment of the purified protein (data not shown). ... MALDI-MS. Biochemical characterization of the LovB KS-MAT didomain Equipped with soluble forms of the LovB KS-MAT didomain and standalone ACP domains, we assayed the catalytic...

Ngày tải lên: 30/03/2014, 08:20