Báo cáo khoa học: Atrial natriuretic peptide-dependent photolabeling of a regulatory ATP-binding site on the natriuretic peptide receptor-A pptx
Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx
... pHYD Pf produced a predominant band with an apparent molecular mass of approximately 56 kDa, which is consistent with the calculated mass of the His-tagged translational product of the corresponding hyd ... 3579 characterization and evaluation of HYD Js from Jannaschia sp. CCS1. Characterization of HYD Js To explore the possible cause of the higher specific activity for...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx
... in addition to an N-terminal Nco1 cloning site. The forward o ligomer 5 ¢-TCCGAAACCAGCG GCCGCTT TATCGCGTTA AAAC CGGT GATCAA ACCCC -3¢ and the reverse oligomer 5¢-GTAGGCCTT TGAATTCCTCAAAA AGTGCGGCTCGAT-3¢ ... chain and hides a large amount of the hydrophobic surface area. Surface area calculations for the pentamer give a total surface area of 81 000 A ˚ 2 with 30% ( 24 00...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf
... O-acetylation of sialic acid may change with transformation or other alteration in the environment of the cell [63]. The normal human tissues contain NeuAc, while malignant tumour cells contain O-acetyl ... Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii Maghil Denis, P. D. Mercy Palatty,...
Ngày tải lên: 21/02/2014, 00:20
Tài liệu Báo cáo khoa học: Purification and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulfide reductase from methanogenic archaea pdf
... overlap). The sequence region upstream o f A F499 is AT-rich and contains typical a rchaeal promoter elements. The sequence AAAGGTTAATATA was f ound 64 bp upstream of the start codon of AF499; ... 3 bp). The region 81–65 bp upstream of t he start c odon of AF499 was identified as an archaeal promoter element by seque nce analysis. The sequ ence AAAGGTTAATATA shows a hig...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot
... DR2: 5¢-CCGTAAGGTCACAAGGTCACTCG-3¢,DR3:5¢-CCG TAAGGTCACAGAGGTCACTCG-3¢, DR4: 5¢-CCGTAA GGTCACAGGAGGTCACTCG-3¢, DR5: 5¢-CCGTAAGG TCACCAGGAGGTCACTCG-3¢. PAL0: 5¢-CGCAAGGT CATGACCTCG-3¢. One strand of each oligonucleotide was annealed after incubation at 100 °C for ... 21-day worms. SmNR1 contains an autonomous transacti- vation function (AF1) in the A ⁄ B domain as demonstrated in a yea...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx
... standard assay mixtures, the velocity of ABTS oxidation was maximal at 45 °C. The dependence of the rate of ABTS oxidation by lac1 on substrate concentration at pH 3.0 and 45 °C followed Michaelis–Menten ... eight separate stages were identified as follows: half-colonization of substrate (day 4), full-colonization (day 8), formation of primordia (day 12), appearance of pinh...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
... primer, 5¢-CATGCCATGGCCAGTAGTCAGCCTGA CCCTACTCCAG-3¢ and 3¢ primer, 5¢-GGTTATCATA TAAAGATCTCAAATTACCC-3¢, for the second PCR the primers were: 5¢ primer, 5¢-GGGTAATTTGAGATC TTTATATGATAACC-3¢ and 3¢ ... medium was obtained. Reduction and alkylation of SSA SSA after Ni–NTA purification was reduced with 10 m M DTT for 2 h at 25 °C. Solid iodoacetamide was then added and alkylation was allow...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
... regulation of caspases. Taken together, these results indicate that VBARP is a novel splice variant of ANKHD1 and may play a role in cellular apoptosis (antiapoptotic) and cell survival pathway(s). Abbreviations ANK, ... VBARP-L consisted of a 576-bp fragment absent from the shorter VBARP-S and contained a 114-bp UTR (Fig. 2A) . Intron and exon analysis of VBARP revealed tha...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Characterization and cDNA cloning of a clofibrate-inducible microsomal epoxide hydrolase in Drosophila melanogaster potx
... the 5¢-end of the ORF and contained a SalIsite(5¢-CTACGTCGACGATGGCGAA CATCTGGCCACGAATC-3¢); the reverse primer corres- ponded to the 3¢-end of the ORF and contained an XbaIsite (5¢-AGGCTCTAGATTTATGAGAAATTGGCTTTCTG GAC-3¢). Expression ... last larval stadium, but it gradually increased, reaching a peak at the wandering stage late in the last larval stadium. At the prepu...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Structural and biological effects of a b2- or b3-amino acid insertion in a peptide Application to molecular recognition of substance P by the neurokinin-1 receptor ppt
... conformational space of b-aminoacidsislargerthanthatofa-amino acids, but low-energy conformations of the b-amino acids backbone, corresponding to gauche rotamers around the Ca–Cb bond, can overlap canonical ... Ac-b 2 -HAla-NHMe and Ac-b 3 -HAla- NHMe indicate that low-energy conformations of the b-amino acids backbone, corresponding to gauche rotamers around the Ca–Cb bond, may...
Ngày tải lên: 08/03/2014, 08:20