Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx
Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx
... indicate that in vitro c-secretase cleavage of APP substrates is inhibited by zinc and that zinc increases the apparent molecular mass of C101-3FLAG as determined by size exclusion chromato-graphy. ... calculated molecular mass. Finally the same line was used to predict the molecular mass of a third FLAG-reactive protein between alpha and c-3FLAGproteins as 11.1 kDa. In vitro c-secretase assays ... of e-C-terminalfragments can be elevated. Lastly, we show that zinc causes an increase in the apparent molecular mass of an amyloid precursor protein c-secretasesubstrate and inhibits its cleavage. ...
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Báo cáo khoa học: In vitro and in vivo self-cleavage of Streptococcus pneumoniae signal peptidase I pot
... blue and destained by 50%methanol. The visualized protein bands on the membranewere excised and the N-terminal amino acid sequence of each protein was determined by automated Edmandegradation. Cleavage ... peptide. This signal sequence is involved in guiding the protein into the targeting and translocatingpathway by interacting with the membrane and othercomponents of the cellular secretory machinery ... itssubstrates. Therefore, the self -cleavage of SPase I is believed to inactivate the activity of the enzyme. In vivoself -cleavage of S. pneumoniaeSPase IAs the in vitro self -cleavage inactivates the...
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Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf
... DDI- 1A and DDI-1B on AAT, CAG and CA repeats are stronger than that of the ATT, CTG and GT strand, which may be attributed to the TÆTmismatch as opposed to the A A mismatch; (f) doxo-rubincin inhibited ... on the CTG strand than the A A mispair on the complementary CAG strand, resulting in expandedCTG fragments that are shorter than those of the CAG strand (Fig. 1E,F). This rule is also the same ... nm caused by base stacking and a negativeband at 250 nm caused by helicity [33], which is char-acteristic of DNA in the right-handed B-form. CDspectra of DNA with DDI- 1A (dashed line) and...
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Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf
... autoradiography visualized by usingGP-Storage Phosphor Screen and storm scanner and software (Amersham Biosciences). Radioactive bands werequantified and annealing percentage was determined by dividing ... knuckle domains(C-/-X-C-G-X3-H-X4-C, where / is an aromatic aminoacid and X is a variable amino acid) typical of retroviralnucleocapsid proteins and the presence of an glycine ⁄arginine rich ... dataindicate that PKA is one of the kinases responsible forCNBP phosphorylation.PKA normally exists in the cytoplasm as an inactivetretameric holoenzyme that is dissociated into twocatalytic...
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Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc
... AnI19R5¢-AATTCATGAGGAGGTTTCTCTGTAACA-3¢;AnI19H5¢-AGCTTGTTACAG-AGAAACCTCCTCATG-3¢; AnI17R5¢-AATTCACGAGGAGGTTTCTCTGTACTA-3¢;AnI17H 5¢-AGCTTAGTACAGAGAAACCTCCTCGTG-3¢;AnI15R5¢-AATTCACAGGAGGTTTCTCT-GTCTA-3¢;AnI15H5¢-AGCTTAGACAGAGAAACCTCCTGTG-3¢. ... this statement and suggests that the RNA binding site is still available and itsbinding affinity is not altered by the presence of DNA in the DNA binding site.Interestingly, when 3 nMRNA and ... RNA to protein is equal to 3 · 109M)1Æmin)1under similar conditions [38] to these. We measured the rateconstant for the binding of DNA to protein kinetically and obtained a value of at...
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Báo cáo khoa học: In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase potx
... power of plasmid A BCFig. 7. Biophysical characterization of the PRAI subdomain. (A) Thermal denaturation of PRAI and trPRAI-His as monitored by CDat 219 nm. Raw data and smoothed curves are shown. ... subjected to an internal duplica-tion of the fifth (ba) unit [24]. Retention of at leasttrace activity in all cases underlined the apparentthermodynamic advantage inherent in the folding of the (ba)8-barrel ... large library of random variantswas also undertaken by plasmid display, to confirm the efficacy of this method for engineering (ba)8-barrelproteins.ResultsStable display of the FLAG epitopeSequence...
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Báo cáo khoa học: In vitro characterization of a plastid terminal oxidase (PTOX) pot
... coli and studied its substrate preferences, the factors in uencing its activity and the nature of suitable metalcofactors and inhibitors in vitro. Materials and methodsChemicals The following ... washing away the chelator by Table 1. Effect of citrate, malate and NaCl on PTOX activity in vitro. Assays were performed in the presence of NADH, decyl-plastoqui-none and KCN as described in the ... presence in the malate and citrate solutions (Table 1). It is therefore probable that, in our assay, inhibition of PTOX activity in the presence of these organic acids is due to the increasing salt concentra-tion...
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Báo cáo khoa học: Gene expression silencing with ‘specific’ small interfering RNA goes beyond specificity – a study of key parameters to take into account in the onset of small interfering RNA off-target effects potx
... statistical analysis The data were normalized in a two-step procedure. First, a correction was applied using a factor calculated from the intensity ratios of internal standards in the test and refer-ence ... samples. The presence of the internal standard probesat different locations of the array allowed quantification of the local background and evaluation of the array homoge-neity, which is taken ... ⁄siRNA2, LAMP2 ⁄ siRNA1 and LAMP2 ⁄ siRNA2 are 5¢-UGACUUCCCUGGCCUAUUUUU-3¢,5¢-ACAUUGAGCUCCUCUCUUGUU-3¢,5¢-GAUAAGGUUGCUUCAGUUAUU-3¢ and 5¢-ACAGUACGCUAUGAAACUAUU-3¢,respectively. As a negative...
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Báo cáo y học: "In vitro bactericidal activities of Japanese rice-fluid against Helicobacter pylori strains"
... in human infections, including H. pylori strains. 2. Materials and Methods Bacterial strains In this study, two standard strains, that is ATCC4350 (type strain) and ATCC43526, and 14 clinical ... Kawakami Y., Akahane T., Yamaguchi M., et al. In vitro activities of rabeprazole, a novel proton pump inhibitor, and its thioether derivative alone and in combination with other antimicrobials ... homogenizing, and were cooled down to 40 to 55°C. In phase 4, they were treated for 10 to 120min., with addition of some amount of proteinase and amylase complex including α-amylase, β-amylase, and...
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BÀI BÁO CÁO KHOA HỌC TẠI BẮC KINH ATCM 2009 THE SCIENTIFIC REPORT IN BEIJING - CHINA
... solve mathematical problems ” Therefore, I have built math-calculator subject and technology in mathematicsWE CAN USE SCIENTIFIC CALCULATORS :1.VIETNAM CALCULATOR VN- 570RS3.CASIO FX-570MS4.CASIO ... t=4.013075236…Introduction•Using a calculator is an important skill for students. As they grow and progress through their education.•They will learn advanced math skills that certainly require them ... them to use a calculator . • A few quick steps can help anyone use the device and solve mathematical problems .•I have organized exams : “Excellent students use scientific calculators to solve...
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Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinQuản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)chuong 1 tong quan quan tri rui roNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)HIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ