... 1.35-m M phosphatase substrate (SIGMA 104Ò, Sigma-Aldrich, Bornem, Belgium) solution was added and the absorbance was measured after 30 and 60 min at 405 nm on a SPECTRAmax PLUS 384 microplate reader system ... coating, the HNE peptide was at least partially oxidized and the signal of the reduced species increased as a result of oxidation. Identification of the active isofo...
Ngày tải lên: 08/03/2014, 08:20
... value analyzed, the period of radiation was somewhat shorter than the value analyzed. This can be assumed that the amount of rise temperature and reaction velocity are accelerated by accumulated ... be decreased and the peak point of hydration heat for the total amount of binder would be increased as increasing the quantity of cement. Therefore, the amount of cement was...
Ngày tải lên: 07/03/2014, 03:20
Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx
... the relaxation data, and N. Srinivasan and S. Yamunadevi for their help with modeling and analyzing the A. thaliana PS protein. References 1 Umbarger E & Brown B (1958) Isoleucine and valine metabolism ... 4A ˚ and 5.5 A ˚ , whereas the backbone Ca atom moves by only 0.9 A ˚ and 0.5 A ˚ , respectively. The backbone Ca atom of Asp35 moves by as much as 1.62...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx
... Ashida M, Minakata H, Oyama H, Oda K, Nishino T & Nakayama T (2004) Crystallographic and biochemical investigations of kumamolisin -As, a serine-carboxyl pep- tidase with collagenase activity. ... enhance- ment of the 57-kDa band with a concomitant diminu- tion of the 38-kDa and 19-kDa bands, as analyzed by SDS ⁄ PAGE (Fig. 3A) . The 38-kDa and 19-kDa bands did not disap...
Ngày tải lên: 19/02/2014, 07:20
Báo cáo khoa học: Alternative splicing: good and bad effects of translationally silent substitutions pdf
... altered isoform proportions, activation of a control mechanism such as nonsense-mediated decay, as well as the creation or loss of splicing variants. As this process has a signifi- cant impact on ... codon information, for an amino acid. Translationally silent variations that affect splicing and disease Clinical studies identifying aberrant splicing mutations are of great...
Ngày tải lên: 06/03/2014, 09:22
Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx
... meth- odology, and recent structural visualization of candi- date proteins by X-ray or NMR structural analyses and the construction of artificial libraries allow con- structive selection and functionalization ... VHH and NCS (Fig. 2A) ; grafting of the CDR 3 loop of antilysozyme VHH functionalized NCS without denaturation, although the thermal stability was decreased and the...
Ngày tải lên: 06/03/2014, 11:20
Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx
... but also osmolytes such as sarcosine and betaine, and the D -enantiomers of serine and alanine. The apparent affinities of PAT1 substrates are mainly in the range of 2–15 m M [1,2,4,6,9]. As shown ... extracellular pH, by determining the apparent K m values of glycine and a lanine at pH values of pH 5.5–8.5 (Fig. 4) and a broad range of membrane potentials. At hyp...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Dual mitochondrial localization and different roles of the reversible reaction of mammalian ferrochelatase ppt
... phospho- ferrochelatase of S13 0A and S30 3A was decreased, and the band was not detected in the double mutant S13 0A and S30 3A, indicating that ferrochelatase was phosphorylated at positions 130 and 303. The reaction of ... S13 0A, S30 3A and S33 0A were prepared: in the first round of PCR, human ferrochelatase [43] was used as a template. Primer pairs used were prim...
Ngày tải lên: 16/03/2014, 00:20
Báo cáo khoa học: Sequences, geographic variations and molecular phylogeny of venom phospholipases and threefinger toxins of eastern India Bungarus fasciatus and kinetic analyses of its Pro31 phospholipases A2 docx
... 5¢-ATGAAAAC TCTGCTGCTGACCTTG-3¢ and primer 2 was 5¢-CTCAA GGAAWTTAGSCAC TCRKAGAG-3¢. For amplification of PLAs, the primers 3 and 4 used were 5¢-GCAGTTTGT GTCTCCCTCTTAGGA-3¢ and 5¢-CACAGTCCTTGA GCTGAAGCTTCTC-3¢. In addition, ... the PCR reactions. The Ala49 mutants are rather unique among the elapid venom PLAs, and mutations of Asp49Ala, Tyr28Asn and Gly30Asp at their catalytic...
Ngày tải lên: 16/03/2014, 12:20
Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf
... complex than in T cells, with several peaks of nuclease hyper- sensitivity [85]. Mast cells express GATA-2 as well as NFAT and AP-1, and GATA-2 initiates the formation of an additional discrete GATA-2 ... elongating Pol II. In addition to yeast SAGA and NuA4, the mammalian HAT HBO1 is also potentially able to be recruited to and to acetylate H4 on K5, K8 and K12, as well...
Ngày tải lên: 14/02/2014, 18:20