Báo cáo khoa học: High thermal and chemical stability of Thermus thermophilus seven-iron ferredoxin Linear clusters form at high pH on polypeptide unfolding doc

... High thermal and chemical stability of Thermus thermophilus seven-iron ferredoxin Linear clusters form at high pH on polypeptide unfolding Susanne Griffin 1 , Catherine L. Higgins 1 , ... stability of FdTt to heat also occurs around neutral pH, and the thermal stability is, like the resistance to chemical denaturation, dramatically reduced at...

Ngày tải lên: 16/03/2014, 23:20

... Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifu- gation, and of granulocytes by combining centrifugation and sedimentation at 1 ... 1-monophosphate (PS 1-MP), tetrasaccharide bisphosphate (TSBP) which lacks the terminal Kdo, and tetrasaccharide 1-monophosphate (TS 1-MP). One-dimensional 1 H- and 31 P-, and two-...

Ngày tải lên: 20/02/2014, 23:20

... of the 15 N– 1 H vectors of the TTF-1 HD core move at the rate of the overall rotational correla- tion frequency, and relaxation mainly occurs as a result of overall rotational diffusion. Among ... and without proton saturation, whereas the cross-relaxation rates, R NOE , were calculated according to the equation R NOE ¼ NOE À 1ðÞ c N c H R 1 ð3Þ The details concerning the relax...

Ngày tải lên: 18/02/2014, 16:20

... of Pro, Val or Lys, c-carboxylation of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different residues. ... spectrum of conomarphin (A) and l-Phe13-conomarphin (B). Fig. S7. Comparison of the three-dimensional struc- tures between conomarphin and l-Phe13-conomar- phin. Table S1. Prot...

Ngày tải lên: 18/02/2014, 17:20

... mL of phosphate buffer, centrifuged again and suspended in 10 mL of phosphate buffer. Serial dilutions of this suspen- sion were plated out on Sabouraud Gentamicin Chloram- phenicol-2-agar plates ... volume of 90 lL of phosphate buffer, added to 100 lL of phosphate buffer containing 5 · 10 3 mid-log growth phase bacteriaÆmL )1 and the mixture was incubated for 3 h at 37 °C...

Ngày tải lên: 18/02/2014, 17:20

... organization suggests a mechanistic coupling of earlier DNA replication steps such as primer synthesis and its elongation and, hence, the autonomy of the plasmid from the host replication apparatus. ... activity on divalent cations was determined using Mg 2+ ,Mn 2+ and Zn 2+ ions at concentrations in the range 0–50 mm. For both studies, Rep245 protein was used at a concentratio...

Ngày tải lên: 18/02/2014, 18:20

... compilation ª 2007 FEBS normal neuronal function [20]. Such considerations indicate that ACMS decarboxylation is a key meta- bolic control step and that the enzyme catalyzing this reaction is ... leads for treating disorders associated with increased levels of quinolinate and ⁄ or picolinate. Experimental procedures PCR amplification and cloning of ACMSD isoforms Human brain, liver...

Ngày tải lên: 19/02/2014, 02:20

... expressed, and purified in Escherichia coli system. The enzymatic characterization of HpSDH demonstrates its activity with k cat of 7.7 s )1 and K m of 0.148 mm toward shikimate, k cat of 7.1 s )1 and ... investigated the catalytic proper- ties of HpSDH and the effects of pH and temperature on HpSDH. The results show that HpSDH has a k cat of 7.7 ± 0.9 s )1 , K m o...

Ngày tải lên: 19/02/2014, 05:20

... major phosphatase responsible for the dephosphorylation and inactivation of S6K [11] and its activity is stimulated upon inhibi- tion of mTor [12]. The main known physiological substrate of S6 ... presence of S6K phosphorylation motifs. An in vitro kinase assay indicated no obvious phos- phorylation of recombinant PDGFR kinase domain by S6K. One could speculate that tyrosine pho...

Ngày tải lên: 19/02/2014, 07:20

... he conformation of backbone had an interproton error of less than 0.2 A ˚ compared to upper and lower boundaries of distances from ROE/NOE data and (b) the conformation had / angles within 30° of ... indicating the stable conformation of the peptide. The Gly3 enantiotopic H a protons were well separated in chemical shift, indicative of stable conformation around Gly3. The NH...

Ngày tải lên: 19/02/2014, 13:20