Báo cáo khoa học: Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase pptx
Báo cáo khoa học: Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase pptx
... Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase Carmine Ercole1, Francesca Avitabile1, Pompea Del Vecchio1, Orlando ... N-terminal arms in BS -RNase maynot be located within the hinge peptide, and point to a crucial role of the interface residues.Keywords: bovine seminal ribonuclease; domain swapping; proline; ribonuclease ... alsoprovides indirect evidence of correct pairing of the inter-chain disulfide bridges in the recombinant sample [11]. The kinetics of the N-terminal arms swapping in the P19A-BS- RNase is comparable...
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Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx
... control the amount of intracellu-lar CagAPY. Cortactin, ezrin and vinculin are all Srcsubstrates, and Src inactivation causes dephosphoryla-tion of these proteins and is crucial for triggering ... distribution,making them unsuitable for the investigation of b-cate-nin signalling [29]. In support of this aspect, usingMadin–Darby canine kidney cells that expressE-cadherin and b-catenin without ... (11). In this manner, the bacteria could access integrins and inject CagA (12). Injected CagA can then induce the massive disruption of cell junctions(13) and a loss of cell polarity (14). The induction...
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Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx
... of R120 by lysine reduces the probability of forming the fully closed form of the protein. The results obtained with the K22V/R120K doublemutant protein point toward a central role of arginine ... the detectability of the peptides of interest. We have used thismethod to show that binding of fosfomycin to the K22mutant proteins strongly depends on the charge of the amino acid side chain occupying this ... Takentogether, our combined calorimetric and protein chemicalapproach leads to a more detailed understanding of the role of K22 and R120 with respect to fosfomycin binding as wellas the ligand-induced...
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Báo cáo khoa học: Role of the C-terminal extension in a bacterial tyrosinase Michael Fairhead and Linda Thony-Meyer doc
... motifArg40Cys84Tyr349Tyr347Lys370Ala36Ala36Ala36Val357Phe518BLaccaseTyrosinaseβ-sheet proteinFig. 1. Overview of the tyrosinase gene and surrounding genes in the genome of V. spinosum. (A) Showing the tyrosinasegene and those in its immediate vicinity in the ... binding motifs common to most tyrosinasesequences [3] that contain five of the six copper-bind-ing histidine ligands. The sixth histidine ligand found in tyrosinases typically occurs before the ... thisligand is most likely histidine 80 in the V. spinosumtyrosinase. Another motif, which is present not only in tyrosinases, but also in the oxygen transportinghaemocyanin proteins, is the PYWDW...
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Báo cáo khoa học: Role of DptE and DptF in the lipidation reaction of daptomycin ppt
... stronglydepends on the nature of the acyl moiety [9,10]. The fine tuning between these two features is of consider-able importance for the development of selectivepotent drugs. The biosynthesis of the peptide ... is GLDSV,indicating that this putative ACP domain is one of the few ACPs in which valine replaces isoleucine (I) orleucine (L) in the conserved sequence. To determinewhether DptF is the putative ... of the nonribosomally synthesized peptide is necessary.Here, we report the results of a steady-state kineticanalysis of DptE. The aim of this kinetic study was todetermine the specificity of...
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Báo cáo khoa học: Role of different moieties from the lipooligosaccharide molecule in biological activities of the Moraxella catarrhalis outer membrane pot
... that the OSs from the wild-type strain consisted of terminal-, 2-, 4-, and 3,4,6-linked glucosyl residues, terminal- and 4-linkedgalactosyl residues, and a terminal-linked N-acetylglu-cosaminosyl ... mutant contained a truncated core OS and consisted of a linker (Glc-Kdo2)-lipid A structure. The phenotype of this mutant was examined to investigate the roles of different moieties of the LOS ... MALDI-TOF-MS of de-O-acylated LOSfrom the mutant and glycosyl composition, linkage, and NMR analysis of its OS indicated that the LOS contained a truncated core OS and consisted of a Glc-Kdo2(linker)-lipid...
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Báo cáo khoa học: Role of Ca2+/calmodulin regulated signaling pathways in chemoattractant induced neutrophil effector functions Comparison with the role of phosphotidylinositol-3 kinase ppt
... whichcatalyzes the production of ROS and results in efficientkilling of invading pathogens [5]. The effect of the calmodulininhibitor W7 on fMLP induced ROS production wasinvestigated and compared with the ... counteracts the calcineurininduced affinity switch of a5b1integrin in CHO cells,suggesting also a role for Ca2+/calmodulin-dependentmolecules in regulating the affinity state of integrins onneutrophils ... calmodulin and PtdIns3K. In guinea pig neutrophils, it has been demonstrated thatinhibition of calmodulin results in inhibition of Rac and p21activated kinases [50]. Involvement of Rac in ROS...
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Báo cáo khoa học: Role of the structural domains in the functional properties of pancreatic lipase-related protein 2 pot
... of the water–lipid interface. Also, both the N-terminal and C-terminal domains of hoPL wereinvolved in the inactivation of the protein at the water–triglyceride interface. The inactivation of ... of the active site was better in the protein bear-ing the N2 domain than in the protein bearing the Ncdomain. Thus, the accessibility of the active site in the N2 proteins was independent of ... investigate the role of the two domains of PLRP2 in the function of the protein, two chimeric proteins were designed by swapping the N and C structural domains between the horse PL (Nc and Ccdomains) and...
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Báo cáo khoa học: Role of CCP2 of the C4b-binding protein b-chain in protein S binding evaluated by mutagenesis and monoclonal antibodies docx
... affected the interaction and taken together the results suggest that the role of CCP2 in the binding of protein S is to stabilize and orient CCP1rather than to provide binding sites for protein S.Materials ... ac-counter.ResultsSelection of amino acids in CCP2 to be mutated In this study, we were interested in investigating whichregions within CCP2 of the C4BP b-chain are involved in protein S binding [13,14]. The mutagenesis ... overlapping binding sites on the b chain, the following four different binding assays using microtiterplates were used. (a) First, the effect of increasing amounts of fluid phase protein S on the binding...
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Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx
... mediator of lethal sepsis, triggering the production of proinflammatory cytokines and liverinjury [13].Therefore, the present study aimed to elucidate the role of KCs in the regulation of CYP isoform ... effects of LPS, play an important role in downregulating hepaticCYP1A2 expression in sepsis [33]. In the present study,increased serum levels of TNF-a and IL-6 and the pro-tein expression of iNOS ... injury, including endotox-emia and ischemia ⁄ reperfusion injury [14]. In ourstudies, we employed GdCl3to inactivate KCs basedon the findings of other investigators showing the destruction of...
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Từ khóa: Báo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Định tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Thơ nôm tứ tuyệt trào phúng hồ xuân hươngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXQuản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)