Báo cáo khoa học: Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase pptx

... Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase Carmine Ercole 1 , Francesca Avitabile 1 , Pompea Del Vecchio 1 , Orlando ... N-terminal arms in BS -RNase may not be located within the hinge peptide, and point to a crucial role of the interface residues. Keywords: bov...

Ngày tải lên: 16/03/2014, 23:20

... control the amount of intracellu- lar CagA PY . Cortactin, ezrin and vinculin are all Src substrates, and Src inactivation causes dephosphoryla- tion of these proteins and is crucial for triggering ... distribution, making them unsuitable for the investigation of b-cate- nin signalling [29]. In support of this aspect, using Madin–Darby canine kidney cells that express E-ca...

Ngày tải lên: 14/02/2014, 19:20

... of R120 by lysine reduces the probability of forming the fully closed form of the protein. The results obtained with the K22V/R120K double mutant protein point toward a central role of arginine ... the detectability of the peptides of interest. We have used this method to show that binding of fosfomycin to the K22 mutant proteins strongly depends on the charg...

Ngày tải lên: 19/02/2014, 13:20

... motif Arg40 Cys84 Tyr349 Tyr347 Lys370 Ala36 Ala36 Ala36 Val357 Phe518 B Laccase Tyrosinase β -sheet protein Fig. 1. Overview of the tyrosinase gene and surrounding genes in the genome of V. spinosum. (A) Showing the tyrosinase gene and those in its immediate vicinity in the ... binding motifs common to most tyrosinase sequences [3] that contain five of the six coppe...

Ngày tải lên: 06/03/2014, 11:20

... strongly depends on the nature of the acyl moiety [9,10]. The fine tuning between these two features is of consider- able importance for the development of selective potent drugs. The biosynthesis of the peptide ... is GLDSV, indicating that this putative ACP domain is one of the few ACPs in which valine replaces isoleucine (I) or leucine (L) in the conserved se...

Ngày tải lên: 07/03/2014, 04:20

... that the OSs from the wild-type strain consisted of terminal-, 2-, 4-, and 3,4,6-linked glucosyl residues, terminal- and 4-linked galactosyl residues, and a terminal-linked N-acetylglu- cosaminosyl ... mutant contained a truncated core OS and consisted of a linker (Glc-Kdo 2 )-lipid A structure. The phenotype of this mutant was examined to investigate the roles of diff...

Ngày tải lên: 07/03/2014, 05:20

... which catalyzes the production of ROS and results in efficient killing of invading pathogens [5]. The effect of the calmodulin inhibitor W7 on fMLP induced ROS production was investigated and compared with the ... counteracts the calcineurin induced affinity switch of a 5 b 1 integrin in CHO cells, suggesting also a role for Ca 2+ /calmodulin-dependent molecules in...

Ngày tải lên: 08/03/2014, 10:20

... of the water–lipid interface. Also, both the N-terminal and C-terminal domains of hoPL were involved in the inactivation of the protein at the water–triglyceride interface. The inactivation of ... of the active site was better in the protein bear- ing the N2 domain than in the protein bearing the Nc domain. Thus, the accessibility of the active...

Ngày tải lên: 16/03/2014, 06:20

... affected the interaction and taken together the results suggest that the role of CCP2 in the binding of protein S is to stabilize and orient CCP1 rather than to provide binding sites for protein S. Materials ... a c-counter. Results Selection of amino acids in CCP2 to be mutated In this study, we were interested in investigating which regions within CCP2 of the...

Ngày tải lên: 17/03/2014, 09:20

... mediator of lethal sepsis, triggering the production of proinflammatory cytokines and liver injury [13]. Therefore, the present study aimed to elucidate the role of KCs in the regulation of CYP isoform ... effects of LPS, play an important role in downregulating hepatic CYP1A2 expression in sepsis [33]. In the present study, increased serum levels of TNF-a...

Ngày tải lên: 14/02/2014, 18:20