Báo cáo khoa học: Cdc37 maintains cellular viability in Schizosaccharomyces pombe independently of interactions with heat-shock protein 90 doc

... FEBS Cdc37 maintains cellular viability in Schizosaccharomyces pombe independently of interactions with heat-shock protein 90 Emma L. Turnbull, Ina V. Martin* and Peter A. Fantes Institute of Cell ... of S. pombe Cdc37, lacking the postulated heat-shock protein (Hsp) 90- binding and homodimerization domains, can also sustain cellular viability, indic...

Ngày tải lên: 16/03/2014, 22:20

... quantification of microgram quantities of protein utilizing the principle of protein- dye binding. Anal Biochem 72, 248–254. 24 Weber K, Pringle JR & Osborn M (1972) Measurement of molecular ... theoretical pI of 7.73; bcp4 encodes a putative pro- tein of 156 amino acids with a predicted molecular mass of 17 461.28 Da and a theoretical pI of 7.65; bcp3 encodes a putati...

Ngày tải lên: 07/03/2014, 05:20

... biochemical wiring within a cell has introduced novel approaches to map the intricate networks of biological interactions arising from the interactions of proteins. The current technologies for assaying protein ... characterization of protein interactions that may provide higher resolution functional data. Common methods for studying protein interactions Current approaches...

Ngày tải lên: 23/03/2014, 15:21

... 6217 Nanoparticles can induce changes in the intracellular metabolism of lipids without compromising cellular viability Ewa Przybytkowski, Maik Behrendt, David Dubois and Dusica Maysinger Department of Pharmacology ... QDs can induce changes in cellular lipid metabolism with- out affecting cellular viability; and (b) QD-induced A B Fig. 5. Uncoated and coated QDs increase...

Ngày tải lên: 07/03/2014, 00:20

... domain inter- acts with an inhibitory phosphotyrosine in the C-terminus. Activation involves dephosphorylation of this inhibitory phosphotyrosine, followed by intermolecular autophosphorylation of ... activities of Src inhibitors and activators, such as C-terminal Src kinase and receptor- type protein tyrosine phosphatases. We demonstrate that C-terminal Src kinase and receptor-ty...

Ngày tải lên: 18/02/2014, 11:20

... duration of overlap seconds of overlap with any other speaker speech rate in A Lexical features: number of words in A number of content words in A ratio of words of A (respectively B) that are also in ... baseline algorithm. 3.2 Maximum Entropy Ranking We view the problem as an instance of statisti- cal ranking, a general machine learning paradigm used for example in...

Ngày tải lên: 20/02/2014, 16:20

... provide information on the local repartition of the angles involved in the SRE fos helix bending. Relative effect of bending strain There are several indications of a redistribution of the strains ... through a mechanism that in uences SRF binding, i.e. by mutual combined interactions of CArG boxes and accessory proteins [21,22]. The core domain of the SRF (core-SRF) binds to...

Ngày tải lên: 07/03/2014, 09:20

... loss in C4H activity, does not affect I2C hydroxylation. As initial cinnamate binding is not in u- enced by this mutation (Table 2) and binding kinetics are first-order (indicating a single binding-site), ... identification of a residue that would be critical for the recognition or positioning of CA. Mutation of all arginines led to a significant loss in protein stability suggesting...

Ngày tải lên: 17/03/2014, 10:20

... transcriptional changes of neuroglobin and cytoglobin in a situation of chronic hypoxia in mice were examined using real-time quantitative PCR. The kinetics of the hypoxic expression of neuroglobin (brain, eyes) ... matter of debate, the estimated local concentration of Ngb in the mouse retina is > 100 lm, and is thus within the range of Mb in the muscle, indicating t...

Ngày tải lên: 23/03/2014, 09:21

... is interesting to note that both forkhead and NHL domains are implicated in protein protein interactions [48,49]. Simi- larly, both bacterial immunoglobulin-like domains and fibronectin type 3 domains ... performed using molar extinction coefficients of 1150 M )1 Æcm )1 at 253 nm. Determination of protein concentration Protein concentration was determined by the Lowry method [21]...

Ngày tải lên: 23/03/2014, 12:20