Báo cáo khoa học: Retention of the duplicated cellular retinoic acid-binding protein 1 genes (crabp1a and crabp1b) in the zebraﬁsh genome by subfunctionalization of tissue-speciﬁc expression doc

... al. Duplicated crabp1 genes in zebraﬁsh FEBS Journal 272 (2005) 35 61 35 71 ª 2005 FEBS 35 71 Retention of the duplicated cellular retinoic acid-binding protein 1 genes (crabp1a and crabp1b) in the ... accepted 16 May 2005)doi :10 .11 11/ j .17 42-4658.2005.04775.x The cellular retinoic acid-binding protein type I (CRABPI) is encoded by asingle gene in mammals. We have characterized two crabp1 genes in ... identiﬁed, the cellular retinol-binding proteins (CRBPs) and the cel-lular retinoic acid-binding proteins (CRABPs). CRBPs,including CRBPI, CRBPII, CRBPIII and CRBPIV,show greatest binding afﬁnity...

Báo cáo khoa học: Suppression of a cold-sensitive mutant initiation factor 1 by alterations in the 23S rRNA maturation region pdf

... StockholmUniversity, S -10 6 91 Stockholm, SwedenFax: +46 8 16 4 315 Tel: +46 8 16 419 7E-mail: Leif.Isaksson@gmt.su.se(Received 24 January 2 011 , revised 1 March2 011 , accepted 14 March 2 011 )doi :10 .11 11/ j .17 42-4658.2 011 .08099.xGenetic ... cold-sensitive IF1FEBS Journal 278 (2 011 ) 17 45 17 56 ª 2 011 The Authors Journal compilation ª 2 011 FEBS 17 51 Immature 23S rRNA termini are present in both the 50S and the 70S fractionsWe then investigated ... cold-sensitive IF1 J. M. Belotserkovsky et al. 17 46 FEBS Journal 278 (2 011 ) 17 45 17 56 ª 2 011 The Authors Journal compilation ª 2 011 FEBSpD1, the e1 () 41) terminus was more prominent. Thus,there were...

Báo cáo khoa học: Structure of coenzyme F420H2 oxidase (FprA), a di-iron ﬂavoprotein from methanogenic Archaea catalyzing the reduction of O2 to H2O ppt

... positioned, the tricyclic F420ring is sand-wiched between the isoalloxazine ring of FMN and the segment between His1 51 and Pro153 of the switchloop, whereby the imidazole group of His1 51 interactswith ... activity. The amino acidsinvolved in FMN binding are highlighted in yellow, and those involved in iron coordination are highlighted in red. The amino acids lining the cavity above the di-iron ... directed towards the prosthetic groups, and the bulky side chains of His1 51 and Trp152 block F420H2binding.Model building of F420H2was governed by the experimentally determined Si-face stereospeciﬁcity...

Báo cáo khoa học: Structures of type B ribose 5-phosphate isomerase from Trypanosoma cruzi shed light on the determinants of sugar speciﬁcity in the structural family ppt

... showedthat a linear sugar molecule was bound (Fig. 3D).Again, the phosphate group interacts with His 11, Arg 113 , Arg137¢ and Arg1 41 . The other end of the substrate points into a deep pocket in the enzyme.Moving ... 24.7 1. 9 (2.00 1. 90) 30.0–2 .1 (2. 21 2 .10 ) 29.4–2 .15 (2.27–2 .15 )Number of reﬂections measured 14 1 283 455 063 2 91 2 91 108 089 13 1 784Number of unique reﬂections 28 418 79 637 63 050 23 17 5 20 ... ligand-free struc-ture) with the complexes with R5P or 4PEH, the moststriking difference is a 1. 5 1. 8 A˚movement of the main chain at residues 10 12 . Asp10 and His 11 inter-act with R5P and...

Báo cáo khoa học: Complexes of Thermoactinomyces vulgaris R-47 a-amylase 1 and pullulan model oligossacharides provide new insight into the mechanism for recognizing substrates with a-(1,6) glycosidic linkages docx

... subsite )1 more deeply than that of P2. This may be caused by Fig. 5. Stereoview of the catalytic site of D356N ⁄ P2. The binding of P2 is shown in green, and the binding of G6 and Phe 313 in D356N ... side chaindepending on the structure of the substrate to formfavorable interactions with the substrate. In the bind-ing of G6, Phe 313 mainly interacts with the six mem-bered ring of Glc–3, ... at the hydrolysing site [17 ,18 ], and subsequently the OD atom of Asp356attacks the C1 atom of the glucose at subsite )1 [19 ].Asp472 has been suggested to be involved in ﬁxing the substrate and...

Báo cáo khoa học: "Effect of intern’s consecutive work hours on safety, medical education and professionalism"

... rapidly and the propensity to err rapidly increases after 16 hours of sustainedwakefulness, a finding reflected in the twofold increase in interns’ attentional failures after they had been working ... intensivecare unit during the studies noted that interns in the intervention schedule “often knew very little about the patients who had been admitted the night before” and that the “intern coming on at ... hand-offsoffset the reduced errors associated with better-rested careproviders? As noted by the authors, their intervention tookplace in a system that was designed to minimize the impactof...

Tài liệu Báo cáo khoa học: Association of feather colour with constitutively active melanocortin 1 receptors in chicken docx

... lLofthesupernatant after addition of [3H]cAMP and bovineadrenal binding protein and incubation for 2 h at 4 °C.Standards containing nonlabeled cAMP were assayed in the same manner. The incubates ... in the humanMC1 receptor and these are over-represented in NorthernEuropean redheads and in individuals with pale skin [17 ,18 ].These variants are a risk factor, possibly independent of skintype, ... expressed in the central regions of the brain,including the hypothalamus where it is an importantregulator of food intake. The MC4 receptor shares 60% of the amino acids with the MC1 receptor,...

Báo cáo khóa học: Characterization of Mesorhizobium huakuii lipid A containing both D-galacturonic acid and phosphate residues ppt

... 3-OH-i -13 :0 16 40 .13 7B 1 + 14 81 – – – 1 1 1 2 1 1 81 P-DAG, 1 · 3-OH-20:0, 1 · 3-OH-i -13 :0,27-OH-28:0, 20:0 14 81. 276B 1 + 11 87– – – 1 1 1 2 1 0 61 P-DAG, 1 · 3-OH-20:0, 1 · 3-OH-i -13 :0,27-OH-28:0 11 86.753Unphosphorylated ... 3-OH-i -13 :0,27-OH-28:0, 20:0 19 82.896B 1 + 14 00– – – 1 0 1 2 1 1 81 DAG2 · 3-OH-20:0,2 · 3-OH-i -13 :0,27-OH-28:0, 20:0 14 01. 297B 1 + 11 08– – – 1 0 1 2 1 0 61 DAG2 · 3-OH-20:0,2 · 3-OH-i -13 :0,27-OH-28:0 11 06.774 13 14 ... 2004,accepted 16 February 2004)Eur. J. Biochem. 2 71, 13 10 13 22 (2004) Ó FEBS 2004 doi :10 .11 11/ j .14 32 -10 33.2004.04038.xNumerous ester-linked nonpolar and (x -1) hydroxyl longchain fatty residues...

Báo cáo khoa học: Construction of a novel detection system for protein–protein interactions using yeast G-protein signaling pdf

... strategy The aim of this study was to establish a rapid and reli-able method for the detection of protein protein inter-actions using yeast G -protein signaling. In our system, protein protein interactions ... BZG 211 8 (ZZmem), BFG 211 8 (Gccyto–Fc) and BZFG 211 8 (ZZmem⁄ Gccyto–Fc) (Table 1) . Expres-sion of ZZmemproteins in BZG 211 8 (lane 2) and BZFG 211 8 (lane 4), or of Gccyto–Fc fusion proteins ... aureus protein A,ZK35Ais a single-site mutant of the Z domain by alter-ing the lysine at position 35 to alanine, and ZI31Ais asingle-site mutant of the Z domain by altering isoleu-cine at...

Báo cáo khoa học: Characterization of a eukaryotic type serine/threonine protein kinase and protein phosphatase of Streptococcus pneumoniae and identiﬁcation of kinase substrates doc

... Most of them were identiﬁeddue to the presence of their genes in the close vicinity of cognate protein kinase genes [12 14 ]. Anotherapproach which could make the identiﬁcation of sub-strates of ... (10 0 %) and compared with the activity of mutated enzyme StkPK42R(lane 2) and StkP in the presence 0 .1 mM,1mM and 10 mM of staurosporine (lanes 3, 4, and 5, respectively), a protein kinase inhibitor. ... lmolÆL )1 [32P]ATP[cP]). The reaction was started by the addition of ATP and terminated after 10 min of incubationat room temperature by adding of 5· SDS sample buffer and analyzed by SDS ⁄ PAGE. After staining and...

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