Báo cáo khoa học: Determinants of the nucleocytoplasmic shuttling of muscle glycogen synthase potx
Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
... is composed of residues 25–540. The overall structure of the model of IPU and the mutated residues in this study are shown in Fig. 3. To elucidate the mechanism of the substrate recognition of IPU, ... panose (data not shown). Prediction of the positions of amino acid residues whose substitutions resulted in the reduction of the IPU activity While the study...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: "Methods for the Qualitative Evaluation of Lexical Association Measures" doc
... we find: (i) Examination of 50% of the data in the SLs leads to identification of between 75% (AdjN) and 80% (PNV) of the TPs. (ii) For the first 40% of the SLs, and lead to the worst results, with ... fre- quency) and the weak measures ( and ). In the second half of the data in the SLs, how- ever, there is virtually no difference between , , and the other measu...
Ngày tải lên: 20/02/2014, 18:20
Báo cáo khóa học: Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy pptx
... direct exploitation of the structural results of the former to explain the mechanism of CaM activation of the latter. Crystallization and resolution of the 3D structures of AC and AC–CaM, which ... coupling Fig. 5. Binding of Ant-dATP to AC (d) and AC–CaM (m). The degree of binding of the ligand was calculated from the value of the ampli- tude of the...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domain pptx
... ring of bacitracin A (937.33). Negative ion mode CID-TOF ⁄ TOF MS analysis of the same precursor yielded the corresponding signature peaks (m ⁄ z 1404.88, 1438.82 and 1470.80) of the other part of ... diminish the immunoblot signals for bacitracin at the site of PDI (Fig. 4C). These data support the view of a robust covalent PDI–bacitracin interaction. We hypothesized...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: Intrabodies against the EVH1 domain of Wiskott–Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells potx
... region. The association of WASP and the Arp2 ⁄ 3 complex activates the actin nucleation activity of the Arp2 ⁄ 3 complex [23]. To investigate further the function of the WASP- EVH1 domain in the ... 5B). Therefore, the EVH1 domain of WASP is not func- tional, at least in the Ag receptor-induced proliferative response of B cells. T cells from the other three 21SHL...
Ngày tải lên: 16/03/2014, 14:20
Báo cáo khoa học: Principles behind the multifarious control of signal transduction ERK phosphorylation and kinase/phosphatase control potx
... how the second kinase in the cascade determines the time dependence of the activity of the third kinase, we varied the V max of the second kinase reaction and recalculated the concentration of the ... are controlled by the kinases or phosphatases in the system, and whether they are controlled differ- entially by some or all of them. On the basis of the an...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: "Search in the Lost Sense of “Query”: Question Formulation in Web Search Queries and its Temporal Change" pptx
... clustering but on understanding the expression of intent. 3 Method We address the main thesis of the work by retrospec- tively studying queries issued to a search engine over the course of 12 consecutive months. Q-queries. ... compute a linear regression of Q-level across 12 months, conduct a hypothesis test (with the null hypothesis being the slope of the regression e...
Ngày tải lên: 17/03/2014, 00:20
Báo cáo khoa học: Studies on the regulatory properties of the pterin cofactor and dopamine bound at the active site of human phenylalanine hydroxylase pptx
... changes of the pro- tomer resulting in a displacement of the cofactor bound at the active site by 2.6 A ˚ (mean distance) in the direction of the iron and Glu286 which causes a loss of the stabilizing hydrogen ... complex, the binding of the cofactor at the active site results in the formation of stabilizing hydrogen bonds between the dihydroxypropyl side-cha...
Ngày tải lên: 17/03/2014, 09:20
Báo cáo khoa học: Alterations in the photoactivation pathway of rhodopsin mutants associated with retinitis pigmentosa potx
... influenced by pH. Rate of Meta II decay as measured by retinal release The rate of retinal release, which parallels the Meta II decay of the protein in the case of the WT under the condi- tions used, ... volume of 650 lL; spectra were normalized to the fluorescence inten- sity of the sample before illumination. For determining the rates of Gt activation, the in...
Ngày tải lên: 22/03/2014, 16:20
Báo cáo khoa học: Curcumin suppresses the dynamic instability of microtubules, activates the mitotic checkpoint and induces apoptosis in MCF-7 cells ppt
... to the pole of the monoastral spindle and also diffused all along the monoastral microtu- bules (Fig. 5B). In the presence of 24 lm curcumin, Eg5 primarily remained confined to the pole of the monopolar ... depolymeriza- tion of the interphase microtubules after 6 h of incuba- tion, suggesting that curcumin exerted a stronger depolymerizing effect on the microtubules...
Ngày tải lên: 23/03/2014, 03:20