... However, while protons from the solvent are incorporated in the course of the pathway, the metabolites can be detected and identified. Therefore adetailed determination of the metabolic process is ... [1]. Inaddition to these natural compounds, several syntheticchemicals contain triple bonds. The amount of theseproducts released to the natural environment in the form of drugs, pesticides, ... isshowninFig.4.Biotransformation of 2-butynedioate The UV spectrophotometric analysis of the 2-butynedioatemetabolism provided scattered absorptions at kmax¼265 nm during the first 24 h of the biotransformation.Furthermore...