... UK). LY294002 and rapamycin were from Calbiochem (Nottingham, UK),genistein from Oxford Biomedical Research (Oxford, MI,USA), PP1 from Biomol (Exeter, UK), SU6656 and phal-loidin from Sigma.Construction ... 236) and anti-src IgG were from Upstate (LakePlacid, NY, USA). Phosphospecific antibody againstp70S6Kinase (pT389) was purchased from Cell Signaling(Danvers, MA, USA). Anti-flag (M2) IgG and ... four dis-tinct bands on a SDS ⁄ PAGE gel due to multiple phos-phorylation. The tyrosine phosphorylated bands ofS6K overlap with two of the activated and slowermigrating bands.We have shown...