Báo cáo khoa học: Aspartate transcarbamylase from the hyperthermophilic archaeonPyrococcus abyssi Insights into cooperative and allosteric mechanisms docx

... 2683 Aspartate transcarbamylase from the hyperthermophilic archaeon Pyrococcus abyssi Insights into cooperative and allosteric mechanisms Sigrid Van Boxstael 1 , Dominique Maes 2,3 and Raymond Cunin 1 1 ... (2003) Aspartate transcarbamylase from the hyperthermophilic archaeon Pyrococcus abyssi: thermostability and 1.8 A ˚ resolution crystal structure of...

Ngày tải lên: 16/03/2014, 19:20

... in the absence ( r)andinthepresenceof50l M (j )and2 00l M (m)ribose 1-phospate. In the insets are shown the replot s o f the slopes and the intercep ts vs. the con centration of t he inhibitor. The ... to the re ciprocal of MTA concentration in the absence (r) and presence of 20 l M (j )and5 0l M (m) adenine. The inh ibition co nstants K is and K ii estimated from...

Ngày tải lên: 16/03/2014, 18:20

... released. Interestingly, the sequence at the cleavage point for the release of the octapeptide is also found at the boundary between the activation peptide and the enzyme moieties. The active enzyme ... of the activation is also lacking the C-terminal peptide. In Fig. 4B the analysis of the species isolated from the chromatograms in part A of the figure confirms...

Ngày tải lên: 17/03/2014, 03:20

... biosynthesized by various OSCs (Fig. 1) and these enzymes regulate the iso- prenoids pathway controlling the biosynthetic flux towards either triterpenoids or phytosterols [11]. Of the members of the ... °CÆmin )1 , and then held at 300 °C for 26 min. The carrier gas was helium and was delivered at a flow rate of 20 cmÆs )1 . The temperatures for the injector and detector...

Ngày tải lên: 23/03/2014, 07:20

... the fifth the RB ORD the very first the JJS the best the RB JJS the very best the ORD JJS the third biggest the RBS JJ the most popular the ORD RBS JJ the second least likely Table 2: The patterns ... extractions. We then randomly sampled and manually la- beled 2, 000 of these extractions for evaluation. We did a Web search to verify the correctness of the sequ...

Ngày tải lên: 23/03/2014, 16:20

... from porcine kidney [29] and bovine liver [30], the enzyme was then purified to homogeneity not only from rat [31] and human liver [32], but also from human lymphoblast [33], from yeast [34] and ... AMP Argininosuccinate is the immediate precursor of arginine leading to the production of urea in the liver and that of NO in many other cells. The importance of both the...

Ngày tải lên: 31/03/2014, 01:20

... site, while the N-terminal domain would contain the cosubstrate and the effector binding sites. The dimerization interface is composed of a-helices and a b-strand from the N-terminal end. The current ... followed the predicted b5-strand (ead///) andprovidesaThrandaValtotheactivesiteofUMP kinases, as well as in aspartokinases and glutamate kinases. This motif comprises t...

Ngày tải lên: 31/03/2014, 07:20

... gambiae TrxR-1 The A. gambiae trxr-1 gene was PCR cloned from genomic DNA as well as from the cDNA of adult insects. In the case of the amplification from genomic DNA, the bases coding for the first ... bands shown on lane 1 in the inset. The N-terminal sequence (17 residues in bold italics) was identified by Edman degradation of the protein isolated from the major ba...

Ngày tải lên: 31/03/2014, 07:20

... Address- ing the question of the nature of this signal and its importance in the fat body metabolic status will reveal interesting information regarding the dynamics of the fat body and provide the opportunity ... 2009 The Authors Journal compilation ª 2009 FEBS their putative functions. Taken together, these data support our hypothesis that our observed amplification of AaTl...

Ngày tải lên: 18/02/2014, 13:20

... occurred from an organelle other than the ER. Internalized saporin and uPA-saporin have not been detected in the Golgi apparatus or the ER [9]. Further- more, saporin intoxication is not affected by the ... increase the pool of saporin available for passage from the endo- somes to the cytosol and would thereby generate an elevated state of toxin sensitivity in the LEX...

Ngày tải lên: 07/03/2014, 05:20