Báo cáo khoa học: Chain initiation on type I modular polyketide synthases revealed by limited proteolysis and ion-trap mass spectrometry doc

... 2387 Chain initiation on type I modular polyketide synthases revealed by limited proteolysis and ion-trap mass spectrometry Hui Hong 1 , Antony N. Appleyard 2 , Alexandros P. Siskos 2 , Jose Garcia-Bernardo 2 , ... readily approach the AT L domain. In conclusion, our strategy for limited proteolysis in combination with on- line liquid chromatography ion tr...

Ngày tải lên: 16/03/2014, 18:20

... charac- teristics of a prothrombinase. This combination of consti- tutive expression and induction may permit a rapid response to inflammation. However, the mechanisms regulating fgl2 expression, either constitutive ... directed mutagenesis. The restriction fragment extending 3.5 kb upstream from the introduced SalI site was excised by digestion with SalIandSmaI (utilizing the SmaI contain...

Ngày tải lên: 20/02/2014, 11:20

... sensitive to inhibition by wortmannin, indicating PI-3¢ kinase is not involved in glucose uptake mediated by these agents [16–18]. Sodium arsenite is known for its atherogenic, carcino- genic and ... phosphorylation of PKC-k on T403. This observation agrees with the absence of PI-3¢ kinase activa- tion by arsenite in vivo (Fig. 3D). Consistent with these observations, no increase of...

Ngày tải lên: 21/02/2014, 00:20

... (amino acids 258–294) binding motifs common to most tyrosinase sequences [3] that contain five of the six copper-bind- ing histidine ligands. The sixth histidine ligand found in tyrosinases typically ... of V. spinosum. (A) Showing the tyrosinase gene and those in its immediate vicinity in the V. spinosum genome. Triangles indicate regions with homology to the binding sites of the E. coli Rp...

Ngày tải lên: 06/03/2014, 11:20

... Ben-Gurion University, Sede-Boker, Israel Nitric oxide (NO) serves as a central signal in a wide variety of processes, including vasodilation, neural communication and immune function in animals ... Arabidopsis catalyze a reaction with arginine that mimics an NOS reaction. This reaction, however, produces argininosuc- cinate, not citrulline, and requires fumarate, indicating that ASL is c...

Ngày tải lên: 07/03/2014, 05:20

... abolished in the pres- ence of actinomycin D, suggesting that, in addition to short-term activation events, TGFb elicits long-term actin cytoskeleton regulation requiring transcriptional induction of ... compilation ª 2008 FEBS RhoB and RhoA promoters and subsequent cloning into the pGL3basic vector at the HindIII (RhoB-Luc) and KpnI–HindIII (RhoA-Luc) sites. The oligonucleotides used a...

Ngày tải lên: 16/03/2014, 06:20

... by incubation on ice. Residual activity was determined by ELISA and the activity half-life (t 1 ⁄ 2, 48 °C ) is defined as the time in which the activity is reduced by 50%. DSC Thermal unfolding ... underlying DNA, leading to transcrip- tional activation or silencing via the recruitment of specific effector proteins. Histone acetylation is the process of acetylation of specific lysine...

Ngày tải lên: 23/03/2014, 06:20

... without the information of the observation sequence (i. e., x x x). The major reason for this simple realization of edge features in traditional CRF implementation is for reducing the dimension ... general purpose optimization method, and it is not limited in the specific task discussed in this paper. 1 Introduction Since Chinese sentences are written as continuous sequences of characters,...

Ngày tải lên: 23/03/2014, 14:20

... through a linguistic analysis based on machine trans- lation, on communication situations and on the users' requirements and satisfaction. I. MACHINE TRANSLATION AND POST-EDITING, A EUROPEAN ... revision, nor correction, nor rewriting. It is a new way of considering a text, a new wav of working on it, for a new aim. In order to define the characteristics of post-ed...

Ngày tải lên: 08/03/2014, 18:20

... hemagglu- tinin. In order to determine the sugar-binding ability and specificity for each of the two sugar-binding sites in EW29Ch, ligand-induced chemical- shift changes in EW29Ch were monitored using 1 H– 15 N ... Gly122, and the side chain nitro- gen and amide proton group of Asn124 in the c sugar- binding site. These residues, which exhibited the most significant perturbations in th...

Ngày tải lên: 23/03/2014, 04:21