Báo cáo khoa học: Identification and characterization of four novel peptide motifs that recognize distinct regions of the transcription factor CP2 doc

... (2005) 1265–1277 ª 2005 FEBS 1277 Identification and characterization of four novel peptide motifs that recognize distinct regions of the transcription factor CP2 Ho Chul Kang 1 , Bo Mee Chung 1 , ... CP2 and YY1 via the HXPR sequence. Mapping the sites on CP2 for interaction with the four distinct CP2- bind- ing motifs revealed at least thr...

Ngày tải lên: 16/03/2014, 18:20

... that there is a relationship between the expression of these transcription factors and the cytokine genes known to be produced by dif- ferent T-cell subtypes in mammals. These transcription factors, ... between the expression of t-bet, stat6 and foxp3 with other genes involved in Th and T reg responses using quantitative PCR. These transcription factors, together with...

Ngày tải lên: 16/02/2014, 09:20

... increased the interaction of SmSmad2 with SmGCN5, but also revealed the interaction of SmSmad4 with SmGCN5 and demon- strated its participation in the formation and, prob- ably, the stabilization of the ... above. Unlike most transcription factors that are sufficient to recruit the basal transcription machinery and therefore activate transcription on both naked...

Ngày tải lên: 18/02/2014, 16:20

... acids at the 5¢ end of the DBD, the DBD and the 40 amino acids at the 3¢ end of the DBD of SmNR1 (Ile247-Ser372) and SmRXR1 (Glu251-Asn376) were tested in vitro. Lanes 1, 5, 9, 13, 17, 21, 25 and ... 6A). The results sugges- ted that both full-length and the A ⁄ B domain of SmNR1 activated transcription of GAL4 reporter gene in the absence of ligand,...

Ngày tải lên: 07/03/2014, 11:20

... nm. The per- centage of H 2 O 2 removed was calculated on the basis of the change in A 490 obtained with Bcp2 relative to that obtained without Bcp2. Thermophilicity and thermoresistance Thermophilicity ... studies have shown that the only cysteine, Cys 49 , present in the Bcp2 sequence, is involved in the catalysis. Lastly, the presence of this Cys in the sequence...

Ngày tải lên: 07/03/2014, 12:20

... analysis of the structure of the latter made it possible to improve the current knowledge of the mammalian PR48 cDNA. To extend these findings, the genomic structures of the human PR130 and PR48 ... GemeenschapÕ, the Fonds voor Wetenschappelijk onderzoek Vlaanderen¢, V. J. and C. V. H. are postdoctoral fellows of the F.W.O. Vlaanderen; I.S. is holder of a postdo...

Ngày tải lên: 08/03/2014, 08:20

... [55]. Expression and purification of the recombinant N-terminal region of FXII and domain D5 of HK Expression vectors for the recombinant N-terminal region of FXII and domain D5 of HK were constructed ... Matsuoka H, Ando K, Watanabe T, Suzuki K et al. (1996) Purification, characterization and cDNA cloning of a novel anticoagulant of the intrinsic path- way, (pro...

Ngày tải lên: 16/03/2014, 05:20

... with the excep- tion of the other peaks in the cysteinylated HSA sam- ple solution. The difference of relative molecular mass of the peak d and the peak g (162.2 Da) and that of peak c and peak ... 91% with the excep- tion of the other peaks in the homocysteinylated HSA sample solution. The difference of relative molecular masses of peaks h and j (1...

Ngày tải lên: 16/03/2014, 13:20

... two protein bands of 40 and 47 kDa, respectively (Fig. 1B). All of these antibodies were raised against regions in the C subunit that are conserved and therefore do not differ significantly between the ... phosphoryla- tion of the nuclear factor of activated T cells [18] and nuclear factor jB [19]. The fact that human T cells express two distinct C subunits (C...

Ngày tải lên: 16/03/2014, 18:20

... or dimerization of the polypeptide chain. The CD spectrum of the mutant was identical to that of the wild-type enzyme, and gel filtration showed that, like the wild-type enzyme, the mutant enzyme ... also propose that the hydropho- bic pocket plays a role in substrate discrimination against binding of the 5-OH group of Kdo and in favour of the N-acetyl g...

Ngày tải lên: 22/03/2014, 21:21