Báo cáo khoa học: Mercury(II) binding to metallothioneins Variables governing the formation and structural features of the mammalian Hg-MT species pptx
Báo cáo khoa học: Mercury(II) binding to metallothioneins Variables governing the formation and structural features of the mammalian Hg-MT species pptx
... Mercury(II) binding to metallothioneins Variables governing the formation and structural features of the mammalian Hg-MT species A ` ngels Leiva-Presa, Merce ` Capdevila and Pilar Gonza ` lez-Duarte Departament ... n the effect of two variables, the reaction time and the presence of chloride ions, on the stoichiometry, stereochemistry and deg...
Ngày tải lên: 16/03/2014, 18:20
Tài liệu Báo cáo khoa học: Calcium-binding to lens bB2- and bA3-crystallins suggests that all b-crystallins are calcium-binding proteins pptx
... cal- cium displaces the dye bound to calcium -binding sites of the protein. Other proteins of this superfamily, namely c-crystallin [20] and AIM1-g1 [15] also induce the J band of the dye indicating ... 492 and 547 nm (Fig. 3). The enhanced luminescence of terbium in the presence of these crys- tallins indicates that Tyr and Trp residues are in the vicinity o...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Covalent binding to glutathione of the DNA-alkylating antitumor agent, S23906-1 doc
... the bionucleophiles DNA and GSH. The covalent binding of the drug to GSH reduced the formation of DNA adducts and slightly decreased the cytotoxic potential of the molecule. There is now little room ... residues at the N2 position in double- stranded DNA [11]. The covalent binding to DNA is apparently responsible for the cytotoxic action [12] and the...
Ngày tải lên: 20/02/2014, 11:20
Báo cáo khoa học: Ibuprofen binding to secondary sites allosterically modulates the spectroscopic and catalytic properties of human serum heme–albumin doc
... 2011 FEBS tion of the heme Fe, as the result of the conforma- tional transition following ligand binding to FA2 [18]. The present considerations appear to apply only to ibuprofen, as other Sudlow ... named the heme site) and the FA2 (corresponding to the regulatory site of the neutral -to- basic conformational transition) pockets display high affinity for FAs...
Ngày tải lên: 06/03/2014, 01:20
Báo cáo khoa học: Nucleotide binding to human UMP-CMP kinase using fluorescent derivatives ) a screening based on affinity for the UMP-CMP binding site potx
... as fluorescent probes to monitor the binding of nucleotides to UCK. These assays were used to determine the binding affinity of bisubstrates and new phosphonate analogs and to validate a fluor- escent ... screening of new compounds. Results and Discussion Competitive fluorescence experiments to determine the binding of natural substrates to the donor and...
Ngày tải lên: 07/03/2014, 09:20
Báo cáo khoa học: Copper-containing nitrite reductase fromPseudomonas chlororaphis DSM 50135 Evidence for modulation of the rate of intramolecular electron transfer through nitrite binding to the type 2 copper center pot
... CoCl 2 ,0.6andNa 2 B 2 O 7 , 0.1. The optical density, the concentrations of nitrate [17] and nitrite [18], the pO 2 and the pH of the medium were monitored, and the composition of the gaseous phase in the fermentor was ... and Shain, and plotted vs. the inverse of the scan rate. The plots yielded straight lines, confirming the applicability of the...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Docosahexaenoic acid stabilizes soluble amyloid-b protofibrils and sustains amyloid-b-induced neurotoxicity in vitro ppt
... charges; in the case of Ab, the arginine at position 5, and the lysines at positions 16 and 28. Interestingly, the formation of fibrils by association of protofilaments has been proposed to involve ... pH. As protofibrils and other oligomeric species of Ab have been found to be toxic [3–5,28,29], and DHA treatment stabilized soluble protofibrils, we examined what e...
Ngày tải lên: 07/03/2014, 10:20
Báo cáo khoa học: 15-Deoxy D12,14-prostaglandin J2 suppresses transcription by promoter 3 of the human thromboxane A2 receptor gene through peroxisome proliferator-activated receptor c in human erythroleukemia cells ppt
... provide further evidence for the role of PPARc in the regulation of TP gene expression within the vasculature and point to further critical differences into the modes of regulation of TPa and TPb ... Similar to the EMSAs involving the extended PPARc ⁄ RXR probe A, nuclear factor bind- ing was independent of 15d-PGJ 2 stimulation. The lig- and independent natu...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Bistability from double phosphorylation in signal transduction Kinetic and structural requirements potx
... e 1 W a ,e 1 W b ,e 2 W b and e 2 W c are the M-M com- plexes. W T and e iT are the total concentration of the interconvertible protein and the total concentration of the catalyst, respectively. Note that only the formation of ... on the ratio between the product of the catalytic constant of cycle 1 (steps 1 and 2) and cycle 2 (steps 3 and 4) (Eqn 7)...
Ngày tải lên: 16/03/2014, 13:20
Báo cáo khoa học: Activator-binding domains of the SWI ⁄ SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo pot
... activation by galactose To determine whether the activator -binding domains of the SWI ⁄ SNF complex are important for its recruit- ment to promoters in vivo and to determine whether the Swi1 and Snf5 domains ... activation of these genes within the same time window. The activator -binding domains in the Swi1 and Snf5 subunits of the SWI ⁄ SNF complex are ess...
Ngày tải lên: 07/03/2014, 00:20