Báo cáo khoa học: Alternative initiation of transcription of the humanpresenilin 1gene in SH-SY5Y and SK-N-SH cells The role of Ets factors in the regulation ofpresenilin 1 pptx

... FEBS 2004 Alternative initiation of transcription of the humanpresenilin 1 gene in SH-SY5Y and SK-N-SH cells The role of Ets factors in the regulation of presenilin 1 Martine Pastorcic 1 and Hriday ... in Ets and Sp1 motifs on PS1promoter activity. The positions of Ets (d) and Sp1 (h)motifsinthe )11 9/ +17 8 region of the PS1 promoter are indic ated. Th e p osi-tions relative to the +1 site of ... fragment SK-N-SH cells SH-SY5Y cells wt m wt m )11 9/ +17 8 10 0 ± 12 21 ± 5 10 0 ± 5 70 ± 20 )11 9/ +14 3 73 ± 10 10 ± 4 13 3 ± 30 70 ± 6)22/ +17 8 91 ± 10 3.4 ± 2.6 10 0 ± 20 28 ± 7)22/+4 23 ± 5 15 0 ± 20...

Báo cáo khoa học: Leishmania infantum LeIF protein is an ATP-dependent RNA helicase and an eIF4A-like factor that inhibits translation in yeast docx

... diffuse into the cytosol of L. donovani infected macrophages and inactivate them[74]. EF-1a plays an important role in eukaryotic pro-tein biosynthesis by binding aminoacyl-tRNAs and positioning them ... 913 –963.36 Pain VM (19 96) Initiation of protein synthesis in euk-aryotic cells. Eur J Biochem 236, 747–7 71. 37 Hershey JWB & MW (2000) Pathway and mechanism of initiation of protein synthesis. ... (P38 919 ) 411 468 71 6.30 55.6 85.9 e )11 8DDX48_Mus (Q91VC3) 411 46840 6.30 55.6 85.9 e )11 8IF42_Hu (Q14240) 407 46402 5.33 56.3 84.6 e )11 7IF42_Mus (P10630)IF 41_ Hu (P60842) 406 4 615 4 5.32 56.1...

Báo cáo khoa học: Alternative splicing: good and bad effects of translationally silent substitutions pdf

... sites) and their proximities (branchpoints). Other cis-acting elements involved in the deter-mination of the splicing outcome are recognized bytrans-acting factors that can either act as splicingsilencers ... aberrant isoforms altering the essential protein func-tion are produced. In this review we brieﬂy describe the current under-standing in the ﬁeld and discuss emerging directions in the study of the splicing ... and further elucidation of this complex and interesting system. Evolutionary studies representanother important ﬁeld for the investigation of the ele-ments involved in splicing regulation and...

Báo cáo khoa học: Alternative splicing: role of pseudoexons in human disease and potential therapeutic strategies pot

... ItalyIntroductionTowards the end of the 19 70s, in the beginning of pre-mRNA splicing research [1, 2], deﬁning exons and introns was essentially based on observing the ﬁnalcomposition of the mature ... is linked to chromosome 12 q23.3-24 .11 and caused by an intron mutation in the ISCU gene resulting in a splicing defect. Hum MolGenet 17 , 16 66 16 72. 11 4 Mochel F, Knight MA, Tong WH, Hernandez ... deletion abolished binding of an U1snRNP molecule in this position and activated a 3¢ss lying 12 nucleotidesupstream of this element. In the last case, binding of hnRNP E1 and U1snRNP to a silencer...

Báo cáo khoa học: Alternative splicing: regulation of HIV-1 multiplication as a target for therapeutic action docx

... sites in SLS3 and in agreement with the strong activationproperties of these proteins on site A3 [29], several of their binding sites overlap the hnRNP A1 bindingsites. However, SC35 binding on the ... further understanding of infection, spread and dissemination through splicing regulation. Such stud-ies coupled with the testing of splicing inhibitors should help the develop-ment of new therapeutic ... thatTat, Rev and their respective RNA binding elementsTable 1. Summary of all data concerning regulatory sites, their position in the genome of HIV -1 BRU strain, factors that bind to them and references...

Báo cáo khoa học: 5-Bromodeoxyuridine induces transcription of repressed genes with disruption of nucleosome positioning pptx

... increases the transcription of repressed genes by disruption of nucleosome position-ing around their promoters. These data will facilitate the understanding of the role of BrdU and AT-tracts in the induction ... positioningWe examined two derivatives of pRS-BAR1, pRS0 01- BAR1 and pRS8 01- BAR1, containing T3CCT6CT5GCT5CT7 and A34, respectively, in a promoter region of the BAR1 gene (Fig. 1A). Insertion ... (Fig. 1) to easily examine the role of BrdU in gene expression and nucleosome positioning in vivo.To further examine the effects of AT-tracts on nucle-osome positioning, we inserted them into...

Báo cáo khoa học: Alternative splicing produces an H-protein with better substrate properties for the P-protein of glycine decarboxylase doc

... naturallyoccuring FtGLDHAA and the artiﬁcial AtGLDH1AA,are indeed exclusively due to the insertion of twoadditional alanyl residues into the N-terminus of the H-protein. In conclusion, the substrate ... NaH 14 CO3(2.5 lCi), 9 lg matrixprotein and 0 10 0 lm H-protein in a total volume of 15 0 lL. H-protein concentrations varied as indicated in the ﬁgures. Reactions were run at 30 °C in the linear ... P-protein) independent experiments.Km (H-protein)(lM)Vmax(nmolÆCO2Æmg )1 Æmin )1 )Mitochondrial P-proteinFtGLDH +4 20.4 ± 2 .1 9.4 ± 0 .1 FtGLDHAA+4 15 .9 ± 1. 6 11 .2 ± 0.4AtGLDH1 + 4...

Báo cáo khoa học: Alternative binding proteins: Anticalins – harnessing the structural plasticity of the lipocalin ligand pocket to engineer novel binding activities pdf

... representation of the crystal structure of the digoxi-genin-binding anticalin DigA16 (PDB entry 1LKE). The bound ligandis shown in a space-ﬁlling representation in yellow, whereas the 16 amino acid ... ﬂuorescein dianion and a Trp sidechain in the binding site of the engineered lipocalin,which closely packs against the xanthenolone moiety[26]. This phenomenon opens interesting applications in ... derivative of digoxigenin) [11 ]. The crystal structures of these ﬁrst anticalins in com-plex with their ligands – and in one instance also asapo-protein – which were solved at resolutions of 2.0...

Báo cáo khoa học: "Alternative Approaches for Generating Bodies of Grammar Rules" docx

... approach(Klein and Manning, 2003) consists in adding headinformation to dependents; words tagged with VBPare then split into classes according to the words thatdominate them in the training corpus.Some ... close to 1 (and 0 MS)for all values of alpha. Hence, there is no needto introduce further distinctions in DT, doing so willnot increase the quality of the automata but will in- crease their number; ... remain hidden in the ﬁnal tree. To clar-ify this, consider the trees depicted in Figure 1; the tree in part (a) is the CW-tree corresponding to the word red big green ball, and the tree in part...

Báo cáo khoa học: Alternative binding modes of an inhibitor to two different kinases doc

... Ile174 (1) C3 Val18(2) Val53(2), Ile174 (1) C4 Leu134 (1) Val53 (1) , Ile66 (1) , Ile174(2)C5 Ala 31( 1), Leu134 (1) Val53 (1) , Ile66 (1) , Ile174 (1) C6 Ile10 (1) , Leu134 (1) , Ala 31( 1) Val53(3), Ile174 (1) C7 ... chain atoms of the hinge region. The N1 atom of the adenine hydrogen bondsto the main chain nitrogen of Leu83 while the N6 grouphydrogen bonds to the main chain oxygen of residueGlu 81. The crystal ... olomoucine [39], roscovitine[40], purvalanol [ 41] and H 717 [42] bound at the ATPbinding site with the purine ring overlapping the siteoccupied by the adenine of ATP but with the purine in aquite...

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