Báo cáo khoa học: Interaction of the E2 and E3 components of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus ppt
Báo cáo khoa học: Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF-1 – a molecular link between recombination and chromatin assembly during meiosis pot
... [8]. The heterodimeric complex of Hop2 and Mnd1 stimulates strand exchange of Lim15/Dmc1 [9–11]. The meiosis-specific proteins Mei5 and Sae3 form a complex with Lim15/Dmc1 and are necessary for the ... to the site of the Lim15/Dmc1–PCNA complex and deposits histone H3 (or a histone vari- ant) and H4 on the naked DNA to restore the nucleosome structure (Fig....
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Estrogen-related receptor a and PGC-1-related coactivator constitute a novel complex mediating the biogenesis of functional mitochondria potx
... FTC-133, XTC.UC1 and RO 82 W-1, the expression of PRC and ERRa was correlated with the mitochondrial mass, the expression of mitochon- drial genes and the activity of the COX and CS enzymes. ERRa ... involved in the metabolic regulation of the three thyroid cell lines We investigated the effects of XCT790, a specific inverse agonist of ERRa. As controls of...
Ngày tải lên: 06/03/2014, 09:22
Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt
... and enzyme–product complexes [29]. On the other hand, k cat ⁄ K m is an apparent second-order rate constant that refers to the properties and the reaction of the free enzyme and free substrate [29]. We have therefore ... published elsewhere. We have therefore measured the thermodynamic parameters of RNase-A in the presence of these amino acids and amino acid derivati...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: X-ray crystallography, CD and kinetic studies revealed the essence of the abnormal behaviors of the cytochrome b5 Phe35fiTyr mutant pdf
... in the side chain conformation of this residue. The shift of the Ca atom of Tyr35 of the Phe35fiTyr mutant from that of Phe35 of the wild-type cyt b 5 is 0.21 A ˚ , within the error limit. The ... [35,36,40,41]. The apo- cyt b 5 and apo-Mb have no absorption in the Soret band region because of the lack of the heme prosthetic group. For the holo-cyt b 5...
Ngày tải lên: 08/03/2014, 10:20
Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
... KIND2 and 43 residues of the mouse MAP2 central domain. Three Leu residues at amino acid positions 461, 474 and 477 in the MAP2-binding core module of KIND2 contributed to the interaction. The ... localized to the N-terminal region, and their specific functional domains are located in the C-termi- nal region. The C-lobe of protein kinases mediates the interaction...
Ngày tải lên: 14/02/2014, 19:20
Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc
... appearance of an additional peak at 7.65 mL on the elution profile. Increase of the temperature of incubation was accompanied by the simultaneous increase of the peak eluted at 7.65 mL and decrease of the ... 0.05, and 0.15 ± 0.06 l M in the absence of HSP25, and in the presence of the wild type HSP25 and its 3D mutant, respectively. At the same time, bo...
Ngày tải lên: 20/02/2014, 23:20
Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx
... more than 50% of the GlnK–TnrA interaction in the presence of 2 mm ATP. On the other hand, 2-oxoglutarate did not influence the GlnK–TnrA interaction, either alone, in the absence of divalent metals, ... affect the binding of ATP to GlnK [12]. Therefore, we investigated the binding of TnrA to GlnK in the presence of different mixtures of Mg 2+ or Mn 2+ with th...
Ngày tải lên: 06/03/2014, 00:21
Báo cáo khoa học: Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) ppt
... Parallel purification of three catalytic subunits of the protein serine ⁄ threonine phos- phatase 2A family (PP2A(C), PP4(C), and PP6(C)) and analysis of the interaction of PP2A(C) with a4 protein. Protein ... with the C-terminal region of the catalytic subunits of PP2A, PP4 and PP6. The TIPRL-interacting region on the catalytic subunit was mapped to residues 21...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles doc
... responsible for the actual membrane fusion step. However, the nature of the interaction of viral fusion proteins with membranes and the mechan- ism by which these proteins accelerate the formation of membrane ... spectra for the E2 peptides showed the characteristics of a random-coil conformation, as indicated by the presence of a negative band at Fig. 1. Fluor...
Ngày tải lên: 07/03/2014, 17:20
Báo cáo khoa học: Interaction of human stefin B in the prefibrillar oligomeric form with membranes Correlation with cellular toxicity doc
... mol ⁄ mol). The results are mean ± SD, n ¼ 1–4. The degree of permea- bilization is expressed as the percentage of the maximal value obtained at the end of the assay by the addition of 2 m M Triton X-100. ... (TEM) and atomic force microscopy [15]. In this study, we measured the interaction of stefin B with various combinations of phospholipid monolayers and b...
Ngày tải lên: 07/03/2014, 17:20