Báo cáo khoa học: Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli potx

... and distribution of nematode protein Ag-NPA-1 FEBS Journal 272 (2005) 180–189 ª 2004 FEBS 189 Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia ... helminth lipid- binding proteins of oad their ligands to membranes: differential mechanisms of fatty acid transfer by the ABA-1 polypr...

Ngày tải lên: 16/03/2014, 18:20

... binding sites of these two AAA-proteins to their different protein regions and elucidated the importance of ATP -binding to the two AAA-cassettes of Pex1p and Pex6p for their inter- action. The amino ... structures and mislocalization of peroxisomal matrix proteins to the cytosol. Here we confirm and extend earlier studies of these AAA-peroxins and give a furth...

Ngày tải lên: 07/03/2014, 16:20

... To further determine the cooperation between the NEG-U and NEG-D elements, 2 lg of the NEG-U decoy, 2 lg of the NEG-D decoy, and a combination of 1 lg of each of the decoys were used, and the above-mentioned ... length between the NEG-U and NEG-D elements on the regula- tion of the Pokemon promoter, we inserted the NEG-U ele- ment into the KpnI ⁄ XhoI...

Ngày tải lên: 30/03/2014, 04:20

... USP7/ HAUSP, and the length of the polyQ region influences the strength of the interaction [163]. Unfortunately, the central part of the Josephin domain is difficult to model because of low sequence ... and the presence of intracellular aggregations of the mutant protein s in affected neurons. In contrast, the expression of the disease-associated genes oc...

Ngày tải lên: 30/03/2014, 15:20

... about the function of the protein. The Fig. 1. (A) Cartoon representation of CagZ protein fold. The L-shape is clearly visible. (B) Two views of the electrostatic potential surface of CagZ. In the ... glutamate and aspar- tate residues confined to the portion of the molecule involving a-helix A, the nearby loop, the first and last turns of helices E and...

Ngày tải lên: 06/03/2014, 00:21

... to rescue the possible damage of the other two disulfide bonds. The presence of a low percentage of the protein with Cys254 and Cys256 in the reduced form further supports this hypothesis. It ... studies the amounts of the protein was adjusted so that no more than 10% of the substrate was converted to product and the reaction rate was strictly linear as a fu...

Ngày tải lên: 23/03/2014, 09:21

... II. The hood domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig. 1A). The two ... atoms, whereas the side chain of Lys173 interacts with both O6 and N7 (Fig. 1C). The 2¢OH of the ribose is hydro- gen bonded to the main chain and the...

Ngày tải lên: 15/02/2014, 01:20

... bonds, one from 2¢-OH to the side chain of Asp70, and one from the backbone N of Gly63, and the phosphate group forming hydrogen bonds from O1 to Arg55, from O2 to the backbone N and the side chain of Thr138, ... E. coli. The probable binding motif for the uracil base is through hydrogen bonds from N3 to the backbone O of Phe133, and from O4 to t...

Ngày tải lên: 18/02/2014, 16:20

... run the typical 5 fold cross validation where we train on 4 folds and test on the 5th and then average the re- sults. In the test setting, we only ran with the best configurations yielded from the ... Surface, where the stem words are left as is with no further processing of the morpho-tactics that result from the segmentation of clitics; (2) Lemma, where the st...

Ngày tải lên: 20/02/2014, 05:20

... induction of the culture with IPTG, which matched the molecular mass predicted from the amino acid sequence of AK2 protein. The purification and functional folding of L. donovani AK from the E. coli cell ... 270) 4345 that of E. coli AK. The flexible LID domain which provides open and closed conformation to the protein during binding of the substrate is...

Ngày tải lên: 21/02/2014, 00:20