Báo cáo khoa học: Effects of cardiomyopathic mutations on the biochemical and biophysical properties of the human a-tropomyosin docx

... fast degradation of IL-1b transcript independently of the presence of AREs (Fig. 6B, lanes 2 and 5). It is possible that in the region located before the AREs, consisting of 343 bp of the 3¢ UTR of the ... lower levels of IL-1b mRNA are due to shortening of the IL-1b transcript half-life, and are not related to the presence of AU-rich elements in the 3¢ U...

Ngày tải lên: 18/02/2014, 14:20

... demonstrated by the biophysical assays. Our studies h ave provided additional contributions to the understanding of the effects of these mutations on the clinical symptoms of patients carrying cardiomyopathic ... indicate that the main effects of mutations A63V, K70T and E180G are mainly related to the overall stability of the protein as a whole, rather...

Ngày tải lên: 16/03/2014, 18:20

... words of the two bunsetsus, and the morphemes at the ends of the bunsetsus (Uchimoto et al., 1999). It is necessary, however, to also consider features for the contex- tual information of the two ... (4) In the transformation from Equation (2) to Equa- tion (3), we assume that the set of noun ns i (T ) is independent of the verb v i (T ). And in the trans- fo...

Ngày tải lên: 20/02/2014, 12:20

... [19] dem- onstrated that phosphorylation of TSC2 by Akt caused the translocation of TSC2 protein from the membrane to the cytosolic fraction. On the basis of these findings, we examined whether or ... IGF-1 stimulation (Fig. 2F). The alteration of the distribution of TSC1 and TSC2 proteins was detected within 10 min of IGF-1 stimulation, which is consistent with th...

Ngày tải lên: 15/03/2014, 11:20

... properties of any particular amino acid segment therein [24]. We compared the localiza- tion of YFP-tagged isoforms A, C and E, containing the L477G, L487G and L515G mutations, with that of the ... understanding of the role of the coiled-coil segment in the differential structure–func- tion properties of the different isoforms, we performed gel filtration exp...

Ngày tải lên: 16/03/2014, 13:20

... FtGLDH AA and the artificial AtGLDH1 AA , are indeed exclusively due to the insertion of two additional alanyl residues into the N-terminus of the H-protein. In conclusion, the substrate properties of the ... differs from the naturally occur- ring Arabidopsis H-protein only by the insertion of two additional alanyl residues near the N-terminus. Kinetic analysis o...

Ngày tải lên: 23/03/2014, 04:20

... co-crystal structure of Bha-RNase H with the sub- strate and metal ions (yellow). The positions of the RNA strand of the substrate with the scissile phosphate group between R()1) and R(0) and two metal ions A and ... conserved in the Sto- RNase HI structure. The weak conservation of the DNA-binding groove may account for the ability of Sto-RNase HI to cleav...

Ngày tải lên: 30/03/2014, 02:20

... enolases and the high degree of sequence conservation, we believe that the effects of these mutations on the structure and function of yeast enolase will be similar to their effects on human bb-enolase. ... position would result in a change in the conformation of this loop. Changes in the conformation of the backbone at this point and changes in the o...

Ngày tải lên: 16/03/2014, 06:20

... extract. Comparison of the optimum concentration conditions between the wild-type and K42T systems We analyzed the dependence of protein productivity on the Mg 2+ and DNA concentrations for the wild-type and K42T ... by the misincorporation of Leu in the poly(U)-dependent poly(Phe) synthesis system. The reaction mixture (30 lL) contained almost all of the com...

Ngày tải lên: 30/03/2014, 13:20

... fraction to a change of the kinase concentration as a function of the concentra- tions of the two forms ([T] and [T*]) and the elastici- ties of the enzymes by the following simple relation: R T Ã K T ¼ ½T e t 2 T Ã ½Tþe t 1 T ½T Ã  ð3Þ As ... of the kinase (G–I). (A) Contour plot of the response coefficient R T Ã K T as function of the total concentratio...

Ngày tải lên: 19/02/2014, 07:20