Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy pdf

... phosphotransferase system in Escherichia coli is a transport and sen- sory system and, in this function, is one of the key players of catabolite repression. Mathematical modelling of signal transduction ... range of different condi- tions, allowing for an extensive analysis of the strains at hand. Dynamics and time hierarchies To show an application of their model,...

Ngày tải lên: 18/02/2014, 13:20

... Nakagawa for access to light scattering, and Miyo Sakai for per- forming ultracentrifugation. This work was supported, in part, by grants-in-aid for scientific research from the Japan Ministry of Education, ... ultra- centrifugation, multiangle laser light scattering and heteronuclear NMR. The results of our analyses allow us to understand the conformational property of EspB and...

Ngày tải lên: 16/03/2014, 18:20

... identified in the first pass analysis were manually interpreted (Table 1). Of the initial 51 tandem mass specta obtained by LC-MS/ MS analysis of the 190 kDa band 45 were selected for a second pass identification ... masses of 33 kDa and 28 kDa, respectively, and two additional protein bands at apparent masses of 42 and 190 kDa [9]. For sequence analysis, the BAP31 immunopreci...

Ngày tải lên: 20/02/2014, 23:20

... synthesis of 15 N-labelled proteins (Eur. J. Biochem. 271) 4093 Optimization of an Escherichia coli system for cell-free synthesis of selectively 15 N-labelled proteins for rapid analysis by NMR spectroscopy Kiyoshi ... preparation of 15 N-labelled samples of hCypA and analysis by NMR spectroscopy, the performance of the cell-free expre...

Ngày tải lên: 16/03/2014, 18:20

... and new compounds with significantly different resistance profiles in particular. Acknowledgements We thank Mrs Terese Bergfors for reading the manuscript and Dr Mats Sandgren for preparation of ... the flaps form a roof over the active site and the ligand. The flaps cover to a large extent the bound ligand. This arrangement is advantageous for the design of inhibitors, because it offer...

Ngày tải lên: 08/03/2014, 02:20

... re- entrancy between the value of agr feature struc- tures at the anchor (verb) node and the subject node. Notice the re-entrancy between the anchor node and the substitution node for the ... instantiates the agr feature of the anchor node as 3sg which has the effect (due to the re- entrancy in the unanchored tree) of instantiating the agr feature at the subject node as 3sg. A...

Ngày tải lên: 08/03/2014, 21:20

... correspondingly enhanced production of H 2 O 2 . A B C D Fig. 5. Activity and cytotoxicity of wt-DAAO and mutants. (A) Com- parison of the activity of wt-DAAO and mutants with 0.1 m MD-Ala and at 2.5% ... estimation of the spectra of intermediates, of rate constants, and of the con- centration of intermediates as a function of time. The same program was used to simulate...

Ngày tải lên: 16/03/2014, 02:20

... A for 5 min, followed by gradients of 0–30% of solution B (66.6% acetonitrile in A) for 5 min, 30–40% B for 30 min, 40–70% B for 5 min, 70–80% B for 10 min, 80–100% B for 5 min, and 100% B for ... seeds of Canavalia ensiformis. J Mol Biol 254, 237–246. 42 Van Scheltinga ACT, Hennig M & Dijkstra BW (1996) The 1.8 A ˚ resolution structure of hevamine, a plant chitina...

Ngày tải lên: 16/03/2014, 13:20

... span a much larger range for the vowel inventories than for the consonant inventories. The significance of the difference in the nature of the distribution of RRs for the vowel and the conso- nant ... inventories of the world’s languages. Through rigorous statis- tical analysis, we find that this redundancy is an invariant property of the consonant in- ventories. The statisti...

Ngày tải lên: 17/03/2014, 04:20

... efficiency. Acknowledgements We thank all members of the laboratory for their sug- gestions and critical reading of the manuscript. This work was supported by grants from the DFG (575 ⁄ 4-1 and TR17-B1) to SG. References 1 ... DHCH and D N mutants in a panel of different cell lines. Although diffi- cult to overexpress, distinct bands corresponding to LIN54 and the DHCH and DN mutants...

Ngày tải lên: 23/03/2014, 04:20