Báo cáo khoa học: Properties of two multifunctional plant fatty acid acetylenase/desaturase enzymes pptx

... alignment of N-terminal (A ⁄ B) domains of LdEcR and DmEcR. (A) Alignment of EcRA from the two species. (B) Alignment of DmEcRB1, DmEcRB2, and LdEcRB. (C) Alignment of most carboxy-terminal side of ... over a stretch of 35–37 amino acids that lie just to the N-terminal side of the DBD (Fig. 1C). The EcRA isoforms from the two species share a few similar motifs in the middl...

Ngày tải lên: 18/02/2014, 08:20

... the formation of half-a- complex or one unit ( one tetramer of GAPDH, one dimer of PRK and one molecule of CP12) essential to the formation of the native complex by dimerization of this unit [29]. ... bi-enzyme complex (460 kDa) which i s made up of two molecules of tetrameric glyceraldehyde-3-phos- phate dehydrogenase (GAPDH) (EC 1.2.1.13), two mole- cules of dimeric ph...

Ngày tải lên: 19/02/2014, 16:20

... T. acidophilum, which shares a high degree of amino acid sequence similarity (60% identity) with the homologous enzyme of P. torridus. Based on the conservation of the zinc-binding sequences of ... the most acidophilic thermophiles known to date [1]. These organisms belong to the order of Thermoplasmales within the Euryarchaeota. Of note, the intracellular pH of Picrophilus ce...

Ngày tải lên: 19/02/2014, 16:20

... rate of accumulation of the two transcripts under these conditions. Induction for longer periods of time did not further change the levels of the two transcripts (not shown). The induced rates of ... the absence of the N-terminal Fe-S centers and the existence of extra sequences in the algal enzymes. To our knowledge, this work represents the first systematic study of expr...

Ngày tải lên: 20/02/2014, 11:20

... alignments of chorismate synthas- es from bacterial, fungal, plant and protozoan origin, of the crystal structure of the enzyme with bound EPSP and of the flavin cofactor, revealed two invariant serine ... those of the wild-type protein. The two- step chromatographic procedure developed for the purification of wild-type enzyme yielded similar amounts of the mutant proteins (3...

Ngày tải lên: 07/03/2014, 05:20

... retain traces of the ancestral properties of cyanobacteria [4]. The formation of b-carotene is one of the most com- mon steps in the synthesis of carotenoids. It requires the cyclization of lycopene ... three genes of the carB operon (Fig. 2) and a part of the fourth gene. Plasmid pMAR162 is similar to pMAR161 but with deletion of a fragment encoding 160 amino acids of t...

Ngày tải lên: 16/03/2014, 05:20

... the sequence properties of their binding sites. HSF4 binding was shown to be closely associated with reduced methylation of the histone H3K9, irre- spective of the relative location of the HSF4-binding regions ... HSF4-binding regions and of the transcriptional status of genes located around the HSF4-binding regions. This result is thus suggestive of a structural effect of H...

Ngày tải lên: 18/02/2014, 04:20

... most efficient of the two (Fig. 3). Correlation between the type of amino acid substitution and cleavage efficiency We analyzed whether there is any correlation between the type of amino acid substitution ... analyzed from this point of view. Substrates containing a silent or tolerated amino acid change Seven of 48 substrates contained a silent amino acid change. However, t...

Ngày tải lên: 07/03/2014, 17:20

... disassembly of IF networks, solubilization of IF proteins, and disruption of desmosomes. Conclusion and perspectives To date, a myriad of studies have exploited the inter- action of DSP toxins ... by ingestion of mollusks. A less well-known effect of these group of toxins is their effect in the cytoskeleton. OA has been shown to stimulate cell motility, loss of stabilization...

Ngày tải lên: 18/02/2014, 14:20

... with 6 lL of Lipofectamine Plus reagent at room temperature for 10 min. Four microliters of Lipofectamine Plus reagent were diluted in 100 lL of Opti-MEM I-reduced serum med- ium. The two solutions ... genera- tion of protein isoforms differing in amino acid sequence. Human DNASE1 pre-mRNA is transcribed from different transcription start sites, exons 1 and 1a, resulting in genera...

Ngày tải lên: 19/02/2014, 06:20