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Báo cáo khoa học: Properties of two multifunctional plant fatty acid acetylenase/desaturase enzymes pptx

Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx

Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx

... alignment of N-terminal (A ⁄ B) domains of LdEcR andDmEcR. (A) Alignment of EcRA from the two species. (B) Alignment of DmEcRB1,DmEcRB2, and LdEcRB. (C) Alignment of most carboxy-terminal side of ... over a stretch of 35–37 aminoacids that lie just to the N-terminal side of the DBD(Fig. 1C). The EcRA isoforms from the two speciesshare a few similar motifs in the middle region of theA ⁄ B ... 1B).Effects of selected agonists on EcR–USPtranscriptional activity in the two speciesIn an initial series of experiments, the basal andligand-induced properties of the three D. melanogasterisoforms...
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Tài liệu Báo cáo khoa học: Involvement of two positively charged residues of Chlamydomonas reinhardtii glyceraldehyde-3-phosphate dehydrogenase in the assembly process of a bi-enzyme complex involved in CO2 assimilation doc

Tài liệu Báo cáo khoa học: Involvement of two positively charged residues of Chlamydomonas reinhardtii glyceraldehyde-3-phosphate dehydrogenase in the assembly process of a bi-enzyme complex involved in CO2 assimilation doc

... the formation of half-a-complex or one unit ( one tetramer of GAPDH, one dimer of PRK and one molecule of CP12) essential to theformation of the native complex by dimerization of this unit[29]. ... bi-enzyme complex (460 kDa) which i s madeup of two molecules of tetrameric glyceraldehyde-3-phos-phate dehydrogenase (GAPDH) (EC 1.2.1.13), two mole-cules of dimeric phosphoribulokinase (PRK) ( ... theother partners of the GAPDH–CP12–PRK complex. Themodel of wild-type GAPDH from C. rein hardtii, like thestructure of spinach GAPDH, shows the presence of agroove containing two positively...
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Tài liệu Báo cáo khoa học: Properties of the recombinant glucose⁄galactose dehydrogenase from the extreme thermoacidophile, Picrophilus torridus ppt

Tài liệu Báo cáo khoa học: Properties of the recombinant glucose⁄galactose dehydrogenase from the extreme thermoacidophile, Picrophilus torridus ppt

... T. acidophilum, which sharesa high degree of amino acid sequence similarity (60%identity) with the homologous enzyme of P. torridus.Based on the conservation of the zinc-bindingsequences of ... the mostacidophilic thermophiles known to date [1]. Theseorganisms belong to the order of Thermoplasmaleswithin the Euryarchaeota. Of note, the intracellular pH of Picrophilus cells of 4.6 is ... the bio-chemical characterization of its product and the effect of zinc ions on the pH and temperature stability of the protein.ResultsAnalysis of the amino acid sequenceMetabolic pathway reconstruction...
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Tài liệu Báo cáo khoa học: Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under anaerobic conditions doc

Tài liệu Báo cáo khoa học: Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under anaerobic conditions doc

... rate of accumulation of the two transcripts under these conditions. Induction for longerperiods of time did not further change the levels of the two transcripts (not shown). The induced rates of ... the absence of theN-terminal Fe-S centers and the existence of extra sequencesin the algal enzymes. To our knowledge, this work representsthe first systematic study of expression of two algal ... sulfurdeprivation. However, the expression of the two transcriptsdiffers during the sulfur-deprivation period. This suggestsdifferences in the regulation of expression of the two genes,also suggested by...
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Báo cáo khoa học: Replacement of two invariant serine residues in chorismate synthase provides evidence that a proton relay system is essential for intermediate formation and catalytic activity docx

Báo cáo khoa học: Replacement of two invariant serine residues in chorismate synthase provides evidence that a proton relay system is essential for intermediate formation and catalytic activity docx

... alignments of chorismate synthas-es from bacterial, fungal, plant and protozoan origin, of the crystal structure of the enzyme with boundEPSP and of the flavin cofactor, revealed two invariantserine ... those of the wild-type protein. The two- step chromatographic procedure developed forthe purification of wild-type enzyme yielded similaramounts of the mutant proteins (3 mg of protein pergram of ... amino acid replacementssignificantly affected the utilization of NADPH as asource of reducing equivalents for activation of thecofactor to its reduced form.Chorismate synthase activity of the...
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Báo cáo khoa học: Cooperation of two carotene desaturases in the production of lycopene in Myxococcus xanthus pot

Báo cáo khoa học: Cooperation of two carotene desaturases in the production of lycopene in Myxococcus xanthus pot

... retain traces of the ancestral properties of cyanobacteria [4].The formation of b-carotene is one of the most com-mon steps in the synthesis of carotenoids. It requires thecyclization of lycopene ... three genes of the carBoperon (Fig. 2) and a part of the fourth gene. PlasmidpMAR162 is similar to pMAR161 but with deletion of afragment encoding 160 amino acids of the coding region of crtIa. ... wouldintroduce two new double bonds, forming trans-lycopene. The high water insolubility of carotenoids,and the improvement of the desaturase efficiency of CrtIa in the presence of CrtIb, suggest...
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Tài liệu Báo cáo khoa học: Roles of heat shock factors in gametogenesis and development pptx

Tài liệu Báo cáo khoa học: Roles of heat shock factors in gametogenesis and development pptx

... the sequence properties of their bindingsites. HSF4 binding was shown to be closely associatedwith reduced methylation of the histone H3K9, irre-spective of the relative location of the HSF4-bindingregions ... HSF4-bindingregions and of the transcriptional status of geneslocated around the HSF4-binding regions. This resultis thus suggestive of a structural effect of HSF4 onchromatin. In the absence of HSF4, histone ... identification of non-Hsp targets, foreshadows a new vision of the role of HSF at a crossroads between stress and development[150,151]. HSF3, which regulates the stress-responsive properties of nonheat...
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Báo cáo khoa học: Adaptation of intronic homing endonuclease for successful horizontal transmission pptx

Báo cáo khoa học: Adaptation of intronic homing endonuclease for successful horizontal transmission pptx

... most efficient of the two (Fig. 3).Correlation between the type of amino acid substitution and cleavage efficiencyWe analyzed whether there is any correlation betweenthe type of amino acid substitution ... analyzed from this point of view.Substrates containing a silent or toleratedamino acid changeSeven of 48 substrates contained a silent amino acid change. However, two of seven such substrates ... Characterization of two intein homing endo-nucleases encoded in the DNA polymerase gene of Pyrococcus kodakaraensis strain KOD1. Nucleic AcidsRes 26, 4409–4412.29 Hanes CS (1932) Studies on plant amylases....
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Tài liệu Báo cáo khoa học: Marine toxins and the cytoskeleton: okadaic acid and dinophysistoxins pptx

Tài liệu Báo cáo khoa học: Marine toxins and the cytoskeleton: okadaic acid and dinophysistoxins pptx

... disassembly of IF networks, solubilization of IF proteins, anddisruption of desmosomes.Conclusion and perspectivesTo date, a myriad of studies have exploited the inter-action of DSP toxins ... by ingestion of mollusks. A less well-known effect of these group of toxins is their effect in the cytoskeleton. OA has been shown to stimulatecell motility, loss of stabilization of focal adhesions ... (35S-methylokadaic acid) cause rapid changes in the structural organization of intermediate fila-ments, followed by a loss of microtubules, solubilization of intermediatefilament proteins, and disruption of...
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Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... with6 lL of Lipofectamine Plus reagent at room temperaturefor 10 min. Four microliters of Lipofectamine Plus reagentwere diluted in 100 lL of Opti-MEM I-reduced serum med-ium. The two solutions ... genera-tion of protein isoforms differing in amino acid sequence. Human DNASE1 pre-mRNA is transcribedfrom different transcription start sites, exons 1 and 1a,resulting in generation of two kinds of ... patients with stable angina pec-toris, offers an in vivo model of mild myocardial ische-mia in humans. Irrespective of a lack of alteration inlevels of other conventional cardiac markers such...
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