Báo cáo khoa học: Puriﬁcation and characterization of Helico

Báo cáo khoa học: Puriﬁcation and characterization of Helicobacter pylori arginase, RocF: unique features among the arginase superfamily doc

... al. (Eur. J. Biochem. 271) Ó FEBS 2004 Purification and characterization of Helicobacter pylori arginase, RocF: unique features among the arginase superfamily David J. McGee 1 , Jovanny Zabaleta 2 , ... necessary and sufficient for arginase activity and has unparalleled features among the arginase superfamily, which may reflect the unique gastric e...

Ngày tải lên : 16/03/2014, 16:20

11
575
0

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc

... composed of two each of the a and b subunits. Analysis of the N-terminal transit peptide of CLGAT Analysis of the CLGAT cDNA predicted a chloroplast transit peptide upstream from the N-terminus of CLGAT. ... the puriﬁcation and characterization of GAT from wild watermelon leaves, and discusses its function during drought ⁄ strong-light stress on the basis...

Ngày tải lên : 20/02/2014, 03:20

12
649
0

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a sialic acid speciﬁc lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... the hemolymph of several crustaceans has been well known since the beginning of the 20th century [34]. An evaluation of the literature revealed that puriﬁcation of lectin from the hemolymph of ... microtiter well and mixed. The values of the HAI titer were determined after 60 min of incubation and expressed as the highest dilution of sugars that inhibited...

Ngày tải lên : 21/02/2014, 00:20

8
616
0

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulﬁde reductase from methanogenic archaea pdf

... out in the presence of one of the substrates of the enzyme. The physiological electron acceptor of the A. fulgidus enzyme is still unknown. Therefore, no substrate could be added to the titration ... Further studies are necessary to elucidate the nature of the extractable heme present in this enzyme. The oxidation of the heme groups of the enzyme by vario...

Ngày tải lên : 21/02/2014, 03:20

10
564
0

Báo cáo khoa học: Puriﬁcation and characterization of zebraﬁsh hatching enzyme – an evolutionary aspect of the mechanism of egg envelope digestion pot

... and E corresponded to the N-termini of the 39 kDa product of ZP2 and the 43 kDa product of ZP3 observed in the 2 min ZHE1 digestion of unfertilized egg envelopes, respectively. By contrast, the ... with those of ZHE1 and MHCE. However, the ratios of the speciﬁc activities of MLCE toward the three substrates were similar to that of ZHE1 and MHCE. In summ...

Ngày tải lên : 07/03/2014, 04:20

13
581
0

Báo cáo khoa học: Puriﬁcation and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt

... Fractionation and puriﬁcation of the enzymes stored in the latex of Carica papaya. J Chrom B 790, 229–238. 32 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage ... content in their latex; and (2) the presence of other cysteine proteinases or isoforms in the latex. To investigate these two hypotheses, the protei...

Ngày tải lên : 07/03/2014, 11:20

12
525
0

Báo cáo khoa học: Puriﬁcation and characterization of cathepsin B-like cysteine protease from cotyledons of daikon radish, Raphanus sativus docx

... in terms of its enzymatic properties. Therefore, TcoCB6 and daikon CBCP are distinct from one another in terms of the role of the one histidine in the occluding loop. As shown in Figs 3 and 4, the ... corresponding to the N-terminal sequence (LPKSFDART) of puriﬁed enzyme and a conserved sequence (AGLPSSKN) in the C-terminal region of the catalytic domain of pla...

Ngày tải lên : 16/03/2014, 04:20

15
426
0

Báo cáo khoa học: Puriﬁcation and characterization of NTPDase1 (ecto-apyrase) and NTPDase2 (ecto-ATPase) from porcine brain cortex synaptosomes doc

... ATP and ADP and the product of ATP and ADP hydrolysis is AMP – the compound with a very low afﬁnity for P2 receptors. The above results conﬁrm the participation of NTPDase1 in the scavenging of ... Cu 2+ and Ba 2+ were strong inhibitors of the analysed enzymes, whereas the presence of monovalent ions in the incubation mixture did not affect the rate of AT...

Ngày tải lên : 17/03/2014, 10:20

8
321
0

Báo cáo khoa học: Puriﬁcation and characterization of three isoforms of chrysophsin, a novel antimicrobial peptide in the gills of the red sea bream, Chrysophrys major doc

... with the DC-protein assay kit using BSA as standard. The concentration of puriﬁed and synthetic chrysophsin-1 and chrysophsin-2 was obtained from the A 280 [24], and that of chrysophsin-3 with the ... difference between the deduced mean molecular mass of the peptide fragment by ESI-ITMS and the theoretical mean molecular mass of the identiﬁed amino-acid sequenc...

Ngày tải lên : 23/03/2014, 20:22

12
482
0

Báo cáo khoa học: Puriﬁcation and characterization of novel salt-active acharan sulfate lyase from Bacteroides stercoris HJ-15 ppt

... roles and structure of the glycoaminoglycans (and proteoglycan), which are involved in the well known anticoagulant activity [7] and the regulation of various cellular processes such as the potentiation ... under the standard reaction conditions. These lyase activities on other sulfated polysaccharides were also measured. One milligram of each substrate was added to the...

Ngày tải lên : 23/03/2014, 21:20

6
400
0