Báo cáo khoa học: Multiple-probe analysis of folding and unfolding pathways of human serum albumin docx

... Multiple-probe analysis of folding and unfolding pathways of human serum albumin Evidence for a framework mechanism of folding Manas Kumar Santra, Abhijit ... (GdnHCl)-induced unfolding of a multidomain protein, human serum albumin (HSA), followed a two-state process. However, using environment sensitive Nile red fluorescence, the unfolding and folding...

Ngày tải lên: 16/03/2014, 16:20

... solvent of the matrix, and 0.5 lL of the mixture was immediately applied to a sample probe spot. Cyclization reaction as a function of pH and temperature In order to determine the effect of pH and ... h. Fig. 2. Comparison of the hydrolysis reaction as a function of denaturing agent and pH. (A), (B) and (C): (d), (s )and( .) correspond to guanidinium chloride concentrati...

Ngày tải lên: 19/02/2014, 12:20

... at 140 (W140A and W140O) was much lower than both the wild-type protein and mutants E142O and K133A. Thermal analysis of protein unfolding The DSC curves of the wild-type protein and the mutants ... melting curves of wild-type and mutants of SNase. DSC curves of five proteins [wild-type (WT), W140A, E140O, W140O and K133A]. The curves of W140A and W140O are nearly...

Ngày tải lên: 20/02/2014, 01:20

... distribution and quantification of the expression of mRNAs of peroxisome proliferator-acti- vated receptors and liver X receptor-a in humans: no alteration in adipose tissue of obese and NIDDM patients. ... the LD itself. Our histocytochemical analysis of the intestine and liver of mice fed Wy-14 643 showed that the induced expression of 17b-HSD11 and formation of L...

Ngày tải lên: 07/03/2014, 05:20

... the co-localization of Rad51 and Lim15/Dmc1 [8]. The heterodimeric complex of Hop2 and Mnd1 stimulates strand exchange of Lim15/Dmc1 [9–11]. The meiosis-specific proteins Mei5 and Sae3 form a complex ... subunit of human CAF-1 (p150) [19] and the largest subunit of Saccharo- myces cerevisiae CAF-1 (Cac1, chromatin assembly complex 1) [20]. The sequence similarities of thi...

Ngày tải lên: 07/03/2014, 05:20

... the C-termini of other members of the secre- tion family, such as PilQ of Myxococcus xanthus [28], ExeD of Aeromonas salmonicida [29], PilQ of P. aerugi- nosa [25,30], and PilQ of N. gonorrhoeae ... function of the competence proteins PilM, PilN, PilO, PilW, PilQ and PilA4 in the DNA translocator of T. thermophilus HB27. Results Heterologous expression and purification o...

Ngày tải lên: 07/03/2014, 12:20

... perturbation of the stability of the protein tertiary structure (although there is about 7% difference of thermal energy change between E129Q and E129G). Discussion Protein folding and unfolding are ... difference of DH cal of mutant from wild-type (%), heat capacity (DC p ) and difference of DC p of mutant from wild-type (%) were determined and the results for the w...

Ngày tải lên: 07/03/2014, 21:20

... biosynthesis and the proliferation of HepG2 cells Jose ´ M. Jime ´ nez-Lo ´ pez, Marı ´ a P. Carrasco, Josefa L. Segovia and Carmen Marco Department of Biochemistry and Molecular Biology, Faculty of Sciences, ... 10 6 cells). Thus we determined the effect of 50 l M and 100 l M doses of HePC upon the contribution of particulate and cytosolic CT to the Table 1. Reversion b...

Ngày tải lên: 17/03/2014, 11:20

... of DNA and the b-ribbon arms, and variable contacts between additional DNA and the body of the protein [19]. In the case of MC1ÆDNA complexes, we know that the protein covers at least 15 bp and ... R 1 relaxa- tion delays of 10, 100, 200, 300, 500, 800, 1000 and 1300 ms and R 2 relaxation delays of 10, 30, 50, 70, 90, 110, 130 and 150 ms at 299 K. Backbone CSPs and 1...

Ngày tải lên: 22/03/2014, 17:20

... mechanisms: cellular and viral mediators of herpes simplex virus entry Jihan Akhtar 1 and Deepak Shukla 1,2 1 Department of Ophthalmology and Visual Sciences, College of Medicine, University of Illinois ... USA 2 Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, IL, USA Introduction The herpesvirus family consists of ove...

Ngày tải lên: 23/03/2014, 04:20