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Báo cáo khoa học: Multiple-probe analysis of folding and unfolding pathways of human serum albumin docx

Báo cáo khoa học: Multiple-probe analysis of folding and unfolding pathways of human serum albumin docx

Báo cáo khoa học: Multiple-probe analysis of folding and unfolding pathways of human serum albumin docx

... Multiple-probe analysis of folding and unfolding pathways of human serum albumin Evidence for a framework mechanism of folding Manas Kumar Santra, Abhijit ... (GdnHCl)-induced unfolding of a multidomain protein, human serum albumin (HSA),followed a two-state process. However, using environmentsensitive Nile red fluorescence, the unfolding and folding pathways of ... GdnHClconcentrations (B) during unfolding (s) and refolding of HSA (d).HSA (2 lM) in the absence of GdnHCl was used as the control.Ó FEBS 2004 Mechanism of folding and unfolding of HSA (Eur. J. Biochem....
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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

... solvent of the matrix, and 0.5 lL of the mixturewas immediately applied to a sample probe spot.Cyclization reaction as a function of pH and temperatureIn order to determine the effect of pH and ... h.Fig. 2. Comparison of the hydrolysis reaction as a function of denaturingagent and pH. (A), (B) and (C): (d), (s )and( .) correspond toguanidinium chloride concentrations of 0 , 2 and 3M, respectively.Hydrolysis ... denaturation curve; DGU14;15;16, free energy of unfolding; DHTm14;15;16, enthalpy of unfolding calculated at Tm; Tm14;15;16,midpoint of the thermal denaturation curve. (Met1)-ONC (M23L),...
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Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf

Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf

... at 140(W140A and W140O) was much lower than both thewild-type protein and mutants E142O and K133A.Thermal analysis of protein unfolding The DSC curves of the wild-type protein and themutants ... melting curves of wild-type and mutants of SNase. DSC curves of five proteins [wild-type (WT), W140A,E140O, W140O and K133A]. The curves of W140A and W140Oare nearly linear in terms of intensity. ... for the study of folding and unfolding pathways. For example, Choy& Kay [22] using NMR15N and 2H spin relaxationtechniques illustrated that the fragment D131D (dele-tion of wild-type...
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Báo cáo khoa học: Regulated expression by PPARa and unique localization of 17b-hydroxysteroid dehydrogenase type 11 protein in mouse intestine and liver pdf

Báo cáo khoa học: Regulated expression by PPARa and unique localization of 17b-hydroxysteroid dehydrogenase type 11 protein in mouse intestine and liver pdf

... distribution and quantification of theexpression of mRNAs of peroxisome proliferator-acti-vated receptors and liver X receptor-a in humans: noalteration in adipose tissue of obese and NIDDMpatients. ... the LD itself. Our histocytochemical analysis of theintestine and liver of mice fed Wy-14 643 showed thatthe induced expression of 17b-HSD11 and formation of LDs are not coupled, at least in ... +Wy14,64317β-HSD11CBB-stainBAFig. 1. Effects of Wy14 643 on protein expression levels of 17b-HSD11 in the liver and intestine of mice. (A) Specificity of theantibodies to peptide (Peptide) and recombinant protein...
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Báo cáo khoa học: Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF-1 – a molecular link between recombination and chromatin assembly during meiosis pot

Báo cáo khoa học: Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF-1 – a molecular link between recombination and chromatin assembly during meiosis pot

... the co-localization of Rad51 and Lim15/Dmc1 [8]. The heterodimeric complex of Hop2 and Mnd1 stimulates strand exchange of Lim15/Dmc1[9–11]. The meiosis-specific proteins Mei5 and Sae3form a complex ... subunit of human CAF-1 (p150) [19] and the largest subunit of Saccharo-myces cerevisiae CAF-1 (Cac1, chromatin assemblycomplex 1) [20]. The sequence similarities of this clonewith human and S. ... cloning of CcCac1L and its interaction with CcLim15. (A) Schematic diagram of the CAF-1 large subunits in human, C. cine-rea and S. cerevisiae. The KER and ED domains are represented by black and...
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Báo cáo khoa học: Identification, subcellular localization and functional interactions of PilMNOWQ and PilA4 involved in transformation competency and pilus biogenesis in the thermophilic bacterium Thermus thermophilus HB27 ppt

Báo cáo khoa học: Identification, subcellular localization and functional interactions of PilMNOWQ and PilA4 involved in transformation competency and pilus biogenesis in the thermophilic bacterium Thermus thermophilus HB27 ppt

... the C-termini of other members of the secre-tion family, such as PilQ of Myxococcus xanthus [28],ExeD of Aeromonas salmonicida [29], PilQ of P. aerugi-nosa [25,30], and PilQ of N. gonorrhoeae ... function of the competence proteinsPilM, PilN, PilO, PilW, PilQ and PilA4 in the DNAtranslocator of T. thermophilus HB27.ResultsHeterologous expression and purification of PilMNOWQ and PilA4To ... The crudeextracts of wild-type (20 lg of protein) and mutant strains (20 lgofprotein each) were analyzed by SDS ⁄ PAGE and western blotting byusing PilM, PilN, PilO, PilW and PilQ antisera....
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Báo cáo khoa học: Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt

Báo cáo khoa học: Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt

... perturbation of thestability of the protein tertiary structure (althoughthere is about 7% difference of thermal energy changebetween E129Q and E129G).DiscussionProtein folding and unfolding are ... difference of DHcal of mutant fromwild-type (%), heat capacity (DCp) and difference of DCp of mutant from wild-type (%) were determined and the results for the wild-type protein and 20mutants ... Calometric melting curves of wild-type and mutants of SNase. DSC curves of nine proteins (WT, E73G, E75G, E101G,E122G, E129G, D77G, D83G and D95G). Lines 3 and 6 representE75G and E129G, respectively;...
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Báo cáo khoa học: Hexadecylphosphocholine inhibits phosphatidylcholine biosynthesis and the proliferation of HepG2 cells pot

Báo cáo khoa học: Hexadecylphosphocholine inhibits phosphatidylcholine biosynthesis and the proliferation of HepG2 cells pot

... biosynthesis and the proliferation of HepG2 cellsJose´M. Jime´nez-Lo´pez, Marı´a P. Carrasco, Josefa L. Segovia and Carmen MarcoDepartment of Biochemistry and Molecular Biology, Faculty of Sciences, ... 106cells). Thus wedetermined the effect of 50 lM and 100 lMdoses of HePCupon the contribution of particulate and cytosolic CT to theTable 1. Reversion by oleate of effect caused by HePC on phosphati-dylcholine ... of HePC can be modulated by the quantity of lipid activator [15], we checked cytosolic CT in the presence of different amounts of PC/oleate liposomes. As can be seenin Fig. 5, the analysis of...
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Báo cáo khoa học: Refined solution structure and backbone dynamics of the archaeal MC1 protein ppt

Báo cáo khoa học: Refined solution structure and backbone dynamics of the archaeal MC1 protein ppt

... of DNA and the b-ribbon arms, and variable contactsbetween additional DNA and the body of the protein[19]. In the case of MC1ÆDNA complexes, we knowthat the protein covers at least 15 bp and ... R1relaxa-tion delays of 10, 100, 200, 300, 500, 800, 1000 and 1300 ms and R2relaxation delays of 10, 30, 50, 70, 90, 110,130 and 150 ms at 299 K.Backbone CSPs and 15N-NOE changes upon ... display and analysis of macromolecularstructures. J Mol Graph 14, 51–55.40 Krzeminski M, Loth K, Boelens R & Bonvin AM(2010) SAMPLEX: automatic mapping of perturbed and unperturbed regions of...
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Báo cáo khoa học: Viral entry mechanisms: cellular and viral mediators of herpes simplex virus entry potx

Báo cáo khoa học: Viral entry mechanisms: cellular and viral mediators of herpes simplex virus entry potx

... mechanisms: cellular and viral mediators of herpes simplex virus entryJihan Akhtar1 and Deepak Shukla1,21 Department of Ophthalmology and Visual Sciences, College of Medicine, University of Illinois ... USA2 Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, IL, USAIntroductionThe herpesvirus family consists of over 100 double-stranded DNA viruses ... proteins and bind a microtubule-dependent, minus end-directedmotor, dynein [9]. Although most of the tegumentproteins are required for activation and modulation of viral gene expression and shut-off...
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