Báo cáo khoa học: Inhibition of Drosophila melanogaster acetylcholinesterase by high concentrations of substrate potx

... and/ or inhibition (diagram not shown). Inhibition of substrate hydrolysis by substrate analogue To get additional information on inhibition by excess substrate, we analyzed the inhibition of substrate ... observed inhibition of the decarbamoy- lation rate by high concentrations of substrate analogue (Fig. 3). The existence of another mechanism for subs...

Ngày tải lên: 16/03/2014, 16:20

... a molecular model of the methyltetra- hydrofolate-binding domain of the rat liver MetS was constructed. In the absence of a high- resolution struc- ture of the methyltetrahydrofolate-binding site ... cycle of cytochrome P450 [29]. Details of the potential binding and electron transfer events that may account for the uncompetitive inhibition of MetS by nitrobenzothiadiazole...

Ngày tải lên: 19/02/2014, 05:20

... situation. Dimerization of C-LytA The occupation of high- affinity binding sites by choline triggers the dimerization of C-LytA [21]. The effect of bicyclic amines on C-LytA oligomerization was ana- lyzed by size-exclusion ... lysis) of the cell wall is an essential process for both cell viability and liberation of viru- lence factors. Inhibition of autolysis by excess c...

Ngày tải lên: 19/02/2014, 05:20

... altered by the presence of the inhibitor, and disrupting the electron transfer between the flavin and the second substrate mainly causes enzyme inhibition by Zn-ferrocyanide. Effect of Fld on the inhibition ... Trippi, V.S. (2000) Inhibition of wheat nitrate reductase a ctivity by zinc. B iol. Plant. 43 , 257–262. 21. Egash ira, T., Takayama, F . & Sakai, K. (2003) Inh...

Ngày tải lên: 19/02/2014, 16:20

... that fivefold higher concentrations of H 2 O 2 needed to be employed to generate a similar r ate and extent of inhibition (Fig. 4 B). Inhibition by protein-derived peroxides was slower than that induced by ... inactivation of G APDH being metal- ion independent, whereas inhibition of GR and LDH by H 2 O 2 occurs most rapidly in the presence of metal ions. It is proposed...

Ngày tải lên: 21/02/2014, 03:20

... level of p-Akt was increased in PrP C -transfected cells. Inhibition of PrP C expression by RNA interference resulted in decreased p-Akt expression. Inhibition of the PI3K/Akt path- way by one of ... in gastric cancer is associated with activation of the PI3K/Akt pathway. Inhibition of PI3K/Akt by LY2940002 or Akt siRNA leads to inhibition of PrP C -induced drug re...

Ngày tải lên: 07/03/2014, 03:20

... the presence of IRE, we measured hydrolysis of [ 32 P]ATP[cP] in the presence of 0–400 ng of IRE (Fig. 8). Compared to the amount of ATP hydrolyzed with 400 ng of IRP alone, addition of IRE increased the ... binding of IRP-1 to IRE, or instead facilitates dissociation of the com- plex. Enhancement of ATP hydrolysis by IRE in the presence of IRP-1, but absence of hydr...

Ngày tải lên: 07/03/2014, 09:20

... in steps of 3) and C-terminal (amino-acids 480–962 in steps of 3 and amino-acids 645–895 in steps of 2) cytosolic domains of hEAG1. Binding of BODIPY FL-labeled hCaM in the presence of 25 lm free ... the C-terminal domain of Ca 2+ -free CaM, i.e. apoCaM. The activation of these channels is medi- ated by conformational changes in the CaM ⁄ SK2 com- plex induced by binding...

Ngày tải lên: 07/03/2014, 12:20

... or by activity in the C-terminal domain of the full-size protein, thereby accounting for the inhibition by ‘acarbose’ reported previously [28]. Our previous studies of the inhibitory effect of ... treatment of Type 2 diabetes [23]. In order to examine the mechanism of action of this class of inhibitors, 3, 5, the stereoisomers of salacinol 6, 7 [25], and the six-membered...

Ngày tải lên: 07/03/2014, 12:20

... assay Transient transfection of Jurkat T-lymphocytes was accomplished by using 5 lL of SuperFect transfection reagent (Qiagen) per ml of culture medium and a total of 1 lg of plasmid DNA. Blank control ... subunit of NF-jB,and measured the level of NF-jB-dependent luciferase gene expression. Transfection of p65 alone resulted in high levels of luciferase expression, wher...

Ngày tải lên: 08/03/2014, 10:20