Báo cáo khoa học: Jackbean, soybean and Bacillus pasteurii ureases Biological effects unrelated to ureolytic activity docx

... Jackbean, soybean and Bacillus pasteurii ureases Biological effects unrelated to ureolytic activity Cristian Follmer, Rafael Real-Guerra, German E. Wasserman, Deiber Olivera-Severo and Ce ´ lia ... share, with jackbean ureases, the property of inducing biological effects not related to their ureolytic activity, we have tested soybean embryo-specific urease...

Ngày tải lên: 16/03/2014, 16:20

... proteins contribute to ER function and protein folding are beginning to emerge, and, importantly, knowledge concerning GmPDIL-3a and GmPDIL-3b can now be applied to the understanding of how divergent ... target these proteins to the A B D C Fig. 3. Expression of GmPDIL-3a and GmPDIL-3b in soybean tissues. (A) Purified recombinant GmPDIL-3a (lanes 1 and 4), GmPDIL-3b (lanes...

Ngày tải lên: 18/02/2014, 11:20

... wild-type and the mutant enzymes with Gly or Gly and THF ⁄ FTHF were monitored using a SX.18MV-R stopped-flow spectrophotometer (Applied Photophysics Ltd., Leatherhead, UK) with a path length of 2 mm and ... corresponding to H122 in bsSHMT, led only to a partial loss of enzyme activity, indicating that it could not be the residue abstracting the proton [14]. In addition, to abstra...

Ngày tải lên: 18/02/2014, 16:20

... cations for activity [6,7]. On the other hand, the type II isomerases of Streptomyces sp. CL190 and Staphylococcus aureus have been reported to require FMN and NADPH in addition to divalent metal ... B. subtilis [52]andthetwoArchaea Methanothermobacter thermoautothrophicus [53] and Sulf- olobus shibatae [54]. These enzymes were found to be dependent on NADPH and Mg 2+ as cofac...

Ngày tải lên: 19/02/2014, 13:20

... Omp1 a nd Omp2 (Table 2). T his ompA DNA was cloned into vector pUC18 and after digestion with Hin dIII and EcoRI, cloned into pDG148 vector to obtain the plasmid pOpac (Table 1). The respective ... cloning vectors and cloned into the vector pQE16 in frame with the His-tag-encoding sequence. The lepB gene was PCR ampli®ed from E. coli DNA using the primers Lep1 and Lep2 (Table 2),...

Ngày tải lên: 21/02/2014, 03:20

... to reserve carbon and nitrogen for germi- nation and early growth [18]. Primary soybean seed storage proteins are globulins called glycinin and b-conglycinin. They are folded and assemble into ... GmPDIL-1 and GmPDIL-2 with proglycinin and b-conglycinin a¢-subunit in cotyledon cells GmPDIL-1 and GmPDIL-2 were shown to have oxi- dative folding activity in vitro and to...

Ngày tải lên: 07/03/2014, 05:20

... displayed purple in color. To determine the effects of pH, temperature and inhibi- tors, l-aspartate and a-ketoglutarate were used as amino donor and acceptor, respectively, and the reactions were performed ... phenylpyruvate. A paper chromatography assay for amino acids was also used to detect the activity toward tryptophan. The reaction was performed as described above, and...

Ngày tải lên: 14/03/2014, 23:20

... All water and acetate atoms were removed. Hydrogen atoms were added to the crystal structure, and the substrates and atomic partial charges were assigned to the protein and car- bohydrate atoms. Atomic ... ‘Ames ancestor’ [BA1557 (NP_844007) and BA3524 (NP_845802)] were identical in size and shared identities of 96% and 95% to BC1534 and BC3461, respectively. The bc1534...

Ngày tải lên: 15/03/2014, 11:20

... time courses and effects of unlabeled ligands and low pH on the dissociation rates of [ 125 I]TC-PCSK9 (A) and [ 125 I]TC-PCSK9-D374Y (B) from HepG2 cells. Binding to equilibrium and removal of ... is in marked contrast to what is seen in many receptor systems, including the insulin receptor [26], where lowering of the pH leads to disso- ciation of ligand from receptor. The re...

Ngày tải lên: 14/02/2014, 14:20

... chaperones (Fig. 1) and are stored in the cytoplasm. However, upon cell contact, the entire cytoplasmic pool of translocator proteins is released rapidly and concurrently, and effectors are translocated ... the production of the pro-inflammatory cytokines tumour necrosis factor-a and interferon (IFN)-c and an over- production of interleukin-10, and it has also been shown to bind...

Ngày tải lên: 14/02/2014, 22:20