Báo cáo khóa học: Membrane transport of fatty acylcarnitine and free L-carnitine by rat liver microsomes pot

... Membrane transport of fatty acylcarnitine and free L -carnitine by rat liver microsomes Jason M. Gooding, Majid Shayeghi and E. David Saggerson Department of Biochemistry and Molecular ... trans-ER membrane transport of palmitoylcarni- tine together with an ER transport process for CoASH, the cosubstrate for microsomal CAT [12]. In support of the notion...

Ngày tải lên: 16/03/2014, 16:20

... N-terminal sequencing.) At 8 h of incubation (lane 2), the bands at 19 and 20 kDa had disappeared, and two clear bands appeared at 15.0 and 13.3 kDa. These bands are thought to be fragments of hTNF-a.At20hof incubation ... After 8 h of incu- bation, the bands at 19 and 29 kDa had disappeared, and two clear bands appeared at 15.0 and 13.3 kDa. These bands are thought to be fr...

Ngày tải lên: 06/03/2014, 22:21

... must be that F , so by static typability, the lemma holds. Intro F and Intro F : and F , so and F are con- sistent. By bounded completeness, F and F . By upward closure, F F and by static typability, F ... unifying the representatives of and , one ( ) for unifying the representatives of and , and one ( ) for unifying the representatives of and . Notice that the...

Ngày tải lên: 08/03/2014, 07:20

... understanding and written consent of the subject) [7] and subsequently maintained in BALB/c mice by intravenous passage every 6 weeks. Promastigotes, obtained by in vitro transformation of liver and ... was terminated by chilling on ice and adding 4 lLof0.25 M PMSF and 60 lLof0.5%(w/v) egg white trypsin inhibitor in 0.15 M NaCl. From the protease treated and untreated...

Ngày tải lên: 21/02/2014, 00:20

... constrained analogues of diacylglycerol (DAG). 27. Modulation of membrane translocation of protein kinase C (PKC) isozymes a and d by diacylglycerol Membrane interactions of diacylglycerol derivatives ... initially to the plasma membrane and only later to the nuclear membrane and other internal membranes (Fig. 1C and D, top rows). Additionally, the PKC-a translocatio...

Ngày tải lên: 15/03/2014, 09:20

... presently of the nature of the FtsY membrane interaction in Archaea. Moreover, the steps leading to deliveryofSRPtothearchaealmembranehaveyettobe described. Towards an understanding of the membrane ... soluble and a membrane- associated protein [11]. While the precise roles and temporal positions of SRP and FtsY in the bacterial SRP cycle remain topics of on-going investig...

Ngày tải lên: 16/03/2014, 16:20

... glycolipid composition of the plasma membrane by altering the ganglioside biosynthetic activity of the cell, while maintaining the normal process of intracellular transport and membrane insertion ... changes of membrane distribution associated with its activation status. EGFr and GD3 localization in CHO-K1 cell membranes Having demonstrated a converse segregation of GD...

Ngày tải lên: 16/03/2014, 16:20

... PAGE (15% gel) and analyzed by WB with mAb against GFP to detect proteolysed fragments of mGFP–calpain 7–Strep. Bands of full-length WT and C290S are indicated by the arrow, and those of 30 K are ... bands were detected by the chemiluminescence method. Membranes were also stained with CBB. Bands of GST– MIT–pHis and GST–pHis in the pulldown products are indicated by ar...

Ngày tải lên: 18/02/2014, 04:20

... NADPH, and 4 mm NDMA in a 1.0 mL final volume. The reaction mixture was incubated at 37 °C for 30 min, and the reaction was stopped by the addition of 0.1 mL of 25% zinc sulfate and 0.1 mL of saturated ... concentrations of digitonin. It is known that low concentrations of digitonin (about 0.05%) selectively damage the outer membrane, and higher concentrations (about 0.1%)...

Ngày tải lên: 18/02/2014, 16:20

... including proliferation, apopto- sis, differentiation, and so on. Isoforms of numerous proteins have been studied and compared in this man- ner, including alternative isoforms of members of the p53 ... Expression constructs were generated encoding Myc-tagged versions of these two isoforms, as well as of the Mxi1-WR isoform that lacks the SID, and shown to give rise to proteins...

Ngày tải lên: 18/02/2014, 16:20