Báo cáo khóa học: Mechanistic insight into the peroxidase catalyzed nitration of tyrosine derivatives by nitrite and hydrogen peroxide doc

... et al.(Eur. J. Biochem. 271) Ó FEBS 2004 Mechanistic insight into the peroxidase catalyzed nitration of tyrosine derivatives by nitrite and hydrogen peroxide Enrico Monzani 1 , Raffaella Roncone 1 , ... °C. The inset shows an expansion of the plot in the low nitrite concentration range. Fig. 2. Inhibition of the LPO-mediated phenol nitration by...

Ngày tải lên: 16/03/2014, 16:20

... works 4453 of the S1-bound chloride anion and ‘pushes’ the Cl ) out of the bottom of the S1 pocket (the occupancy of the Cl ) in this structure is 0.7). The alternate confor- mation of the Asp443 ... with the occupancy of the S1 pocket by a ligand and the ‘open–close’ transition exploits the hinges on the two sides of the lid made up of Asn540...

Ngày tải lên: 23/03/2014, 05:22

... erva- tamin-A, the P3 moiety of both the molecules of the asymmetric unit runs along the extended backbone of residues 65–64 and is partly exposed to the solvent (Fig. 2B). On the other hand, the P3 ... proton from the catalytic Cys pres- ent at the N-terminus of the helix to the His of the dyad [19,50,51]. The first stage of catalysis is mediated by...

Ngày tải lên: 18/02/2014, 16:20

... enveloped by subunit 7. The arrows labeled (b) and (c) point to the areas of interaction between the transmembrane helix of subunit 8 and helices G and H1 of cytochrome b and between the N-terminus of ... membrane or lack of detection of the truncated protein by the antibodies. In the W303–1B q° strain the amounts of the other two catalytic subunits...

Ngày tải lên: 19/02/2014, 12:20

... plate- lets and endothelial cells. The important role of these glycans in pharmacokinetics has been demonstrated by the slower and smaller increment of FDP in vivo after the injection of desialylated ... the b-Man residue at the C4 position. Finally, an H ion would be formed through concerted loss of the substituents on the 6-arm and the bisecting GlcNAc. The...

Ngày tải lên: 07/03/2014, 06:20

... the cytosol. This observation allows the further determination of the concentration of drug free in the cytosol of resistant cells. The determination of k a requires also the measurement of the ... the rate of passive diffusion of the dye through the plasma membrane. This cannot be done by the simple measurement of the increase of the fluorescence...

Ngày tải lên: 08/03/2014, 08:20

... motif, they are both targeted to the nuc- leus, and they bind to and become activated by DNase I-treated DNA. Both PARP-2 and PARP-1 can homo- and heterodimerise, and both are involved in the base excision ... by many groups. Attractive scenarios developed over the last couple of years are (a) the localized relaxation of chromatin at the site of DNA damage, media...

Ngày tải lên: 20/02/2014, 01:20

... conditions. The redox state of the PETN crystals could easily by assessed and verified by bright yellow colour of the oxidized crystals vs. the colourless reduced form. All data were processed and scaled ... above the si face of the FMN isoalloxa- zine ring and are held in position by hydrogen bond interactions with His181 and His184 [2]. These residues also f...

Ngày tải lên: 20/02/2014, 02:21

... mechanism of antitumour action of BS-RNase is based on the ability of the protein to resist the neutralizing action of the cytosolic RNase inhibitor, a resistance due to the dimeric structure of the enzyme. ... approaches [4], and explained [4–6] by the resistance of the enzyme in its dimeric state to the inhibitory action of CRI (the cytosolic RNase inhib...

Ngày tải lên: 20/02/2014, 11:20

... investigated the biological activities of these neuropeptides in the crab Carcinus maenas, at the target tissue, receptor and cellular level by bioassay (inhibition of ecdysteroid synthesis), radioligand ... MIH or CHH during the moult cycle is not at the receptor level. In view of these observations, we also investigated levels of MIH and CHH peptides in the sinus g...

Ngày tải lên: 21/02/2014, 00:20