Báo cáo khóa học: Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding pdf

... of metal binding to arginase II To further examine the effects of the His120fiAsn and His145fiAsn mutations on the interaction of the enzyme with manganese ions, maximally activated spe- cies of ... bound to a binuclear metal cluster and, consequently, by a higher concentra- tion of the nucleophilic metal- bound hydroxide [33–35] and increased stabilization of the tran...

Ngày tải lên: 20/02/2014, 02:21

... process of state transitions represents a short-term adaptation of the photosynthetic apparatus to the con- ditions of imbalanced illumination of PSII or PSI. It occurs on a timescale of seconds ... conditions of over- excitation of PSI (or preferential illumination with light 1), oxidation of the PQ pool occurs followed by de-activation of LHCII-specific kinase(s) and de...

Ngày tải lên: 16/03/2014, 06:20

... cDNA of Chang cells, and the PCR product was sequenced. (D) PCRs for exon 1 and exon 1a were performed in Huh7 and Chang cells. (E) The boundary regions of exon 8 and exon 9 in the genomic DNA of ... apoptosis and the inhibition of invasion, along with the downregulation of matrix metalloproteinase expression. In addition, by using the calcium indicator X-rhod-1, and the...

Ngày tải lên: 18/02/2014, 18:20

... transcription–PCR analysis of unknown serial analysis of gene expression tags; rSAGE, reverse serial analysis of gene expression; SAGE, serial analysis of gene expression; TSAT-PCR, two-step analysis of ... PCR analysis of unknown tags on serial analysis of gene expression Wang-Jie Xu 1 , Qiao-Li Li 1 , Chen-Jiang Yao 1 , Zhao-Xia Wang 1 , Yang-Xing Zhao 1 and...

Ngày tải lên: 07/03/2014, 04:20

... starting values of the concentra- tions and fluxes remain unaltered (as previously). Inhibition of the AKI and AKII activities makes the main contribution to the generation of the control pat- tern ... by Lys (G-III) on the flux control pattern for large responses. Fig. S3. Contributions of the inhibitions of the AK and HSDH activities (of bifunctional AK-HSDH) by Thr to the...

Ngày tải lên: 14/03/2014, 23:20

... kinases and phosphata- ses, which control the activation state of signaling pathways by the modulation of phosphorylation on Tyr, Ser and Thr residues on each other and on a vari- ety of downstream ... effects of kinase inhibition on the phosphorylation status of pathway components may be obtained using a compound titra- tion series (Fig. 1). A concentration–response...

Ngày tải lên: 16/03/2014, 00:20

... Under such conditions, an increasing dependence of k on substrate concentration from 0 to k ¼ k es + k )es would be expected. So far, such a dependence of k on substrate concentration has only been ... step appears to be the result of a combination of steric con- straints and impaired electronic effects of the ethereal sulfur atom on stabilization of the acylation tran...

Ngày tải lên: 07/03/2014, 14:20

... was normalized based on protein concentrations. FACS analysis and cytokine displacement BAF GLC and SK-N-GP cells were incubated in the presence of increasing concentrations of putative competitor (CC–FP, ... demonstrate the involve- ment of the PI3-kinase/AKT and MAP kinase signaling pathways in functional responses to the CC–FP fusion cytokine. DISCUSSION We have demonstrate...

Ngày tải lên: 08/03/2014, 10:20

... transition and functional behaviour upon interaction with uPA and several side chains involved in the retardation of latency transition, and hence of importance for the functional stability of stressed ... Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of...

Ngày tải lên: 20/02/2014, 11:20

... the corresponding nitriles. NHase is a metalloenzyme that contains iron or cobalt in its catalytic center. Iron-containing (Fe-type) NHase contains a nonheme iron ion [3,4], and cobalt-containing (Co-type) ... form and size of the hydrophobic pocket Fig. 5. Superimpositioning of the metal centers of the wild-type enzyme and mutants of the cobalt-containing nitrile hydra...

Ngày tải lên: 16/03/2014, 16:20