Báo cáo khóa học: How calcium inhibits the magnesium-dependent enzyme human phosphoserine phosphatase potx

... Belgium The structure of the Mg 2+ -dependent enzyme human phosphoserine phosphatase (HPSP) was exploited to examine the structural and functional role of the divalent cation in the a ctive site of phosphatases. ... How calcium inhibits the magnesium-dependent enzyme human phosphoserine phosphatase Yves Peeraer 1 , Anja Rabijns 1 , Jean-Franc¸ois ... atoms...

Ngày tải lên: 16/03/2014, 16:20

... that the subunit with the highest affinity for the substrate almost has the same affinity for the product (the K I1 values are only slightly lower than the K S1 values), while the subunit with the ... M1 site of the inactive subunit to the M2 site of an active subunit [20,22], the enzyme is expected to be present in the form containing two Zn 2+ ions on the same mon...

Ngày tải lên: 21/02/2014, 00:20

... orientation of the side chains, replacing P300 with a serine would leave the H-bond to the OH group of the side-chain nonsatis- fied, thereby destabilizing the protein. Based on the CPB structure, ... above. Determination of the half-life (T1/2) of CPU’s activity The T1 ⁄ 2 of the activity of the best mutants secreted in the supernatant of the cells in both screens was...

Ngày tải lên: 07/03/2014, 12:20

... architecture of the dimer is reminiscent of the human IMPase (Fig. 1, right panel). However, the dimer interface is quite dif- ferent from the human enzyme and other nonarchaeal IMPases. The superposition ... enclosed by the solid boxes. The contact on the left hand side comprises the active site loop (residues 22–42), shown in detail in Figs 3 and 4. At the other si...

Ngày tải lên: 16/03/2014, 11:20

... string- based in the sense that they base the translation de- cision on the words and their surrounding context. Contrary, in the field of syntax-based machine trans- lation, the translation models ... the rank rk(t, u) of the pre-translation and the nonterminals in the right-hand side that are aligned to p should be replaced by the translation that the alignment reques...

Ngày tải lên: 17/03/2014, 00:20

... have shown that the two proteases are essen- tially concentrated in the myofibrils near the Z-disk and, to a lesser extent, in the I-band [64–66]. Their presence has also been reported under the ... be another option. The numer- ous possible targets in cells (Table 1), the broad spectrum of the cleaved sequences, and the fact that the two ubiquitous isoforms can substitute...

Ngày tải lên: 23/03/2014, 10:21

... released by the Linguistic Data Consortium (LDC). We treated the human alignment as the oracle of supervised alignment. The re- sult is surprising: the gain of human alignment over a state of the ... over GIZA++ is small on the small corpus. One may wonder whether the small magnitude of the improvement is an artifact of the small size of the training corpus. To...

Ngày tải lên: 23/03/2014, 16:20

... that either the phosphatases are inactivated or they are unable to access their target, perhaps due to altered trafficking or sequestration of the phosphorylated ERK1/2. Many ERK- directed phosphatases, ... effects of the inhibitors are not a factor. However, the possibility of other kinases also being affected by the inhibitors cannot be excluded. Molecular manipulation of select co...

Ngày tải lên: 30/03/2014, 13:20

... concluded that the inherent flexibility of these LA derivatives allows the large conformational changes required to form the cross-b-structure of the amyloid fibrils. Of interest, it is also shown that the ... results from the replacement of labile protons by deuterium. The fea- tures of the H ⁄ D exchange of the low-pH MG of LA have been already examined [43–46], showing that...

Ngày tải lên: 30/03/2014, 16:20

... near the N- and C-termini of the linkers: this suggests that a mobile connection with both the catalytic domain and the binding module is required for the function of the nanomachine. Furthermore, ... etc.), the pK a difference between heteroatoms is of importance: the smaller this difference, the stronger the hydrogen bond, as the hydrogen atom is equally shared between...

Ngày tải lên: 14/02/2014, 18:20