Báo cáo khoa học: Characterization of rat cathepsin E and mutants with changed active-site residues and lacking propeptides and N-glycosylation, expressed in human embryonic kidney 293T cells ppt

... rat cathepsin E mutants in which active-site Asp residues were changed to Ala or which lacked propeptides and N-glycosylation. Wild-type cathepsin E expressed in human embryonic kidney 293T cells ... propeptide and ER-retention sequence. (A) Schematic representation of the structures of the mutants lacking the propeptide and most of the ER-r...

Ngày tải lên: 16/03/2014, 14:20

... pool sequencing of the mixture o f CNBr pep tides generated from CLAP revealed in the C-terminal CNBr peptides, the presence of N299, G300, and V 301 i n s equence c ycles 31–3 3, with V301 being the ... room temperature, the complex wasextracted with 1 mL n-butanol and the color intensity determined by analysis at 520 nm. The number of pmoles of cathepsin L were determine...

Ngày tải lên: 07/03/2014, 16:20

... Cardiosuppressive effects (i .e. negative inotropy) of N-terminal CGA-derived peptides have been well documented in vertebrate heart [31]. In particular, in the isolated working heart of the frog Rana esculenta, ... CGA-derived peptides present in rat heart extract Experiments were carried out to determine whether acidic or heat treatment of rat heart was able to enrich and...

Ngày tải lên: 07/03/2014, 12:20

... homodimer in the same organ. The structural differences between rIAPs observed in the crown A B Fig. 4. Stereoviews of the residues near metal-binding site 3 in (A) rat intestinal alkaline phosphatase-I ... rIAP-II, respectively [11,23]. The rIAP-I gene is expressed in both duodenal and jejunal mucosa, whereas the rIAP- II gene is expressed only in duodenal mucosa [17,18...

Ngày tải lên: 07/03/2014, 17:20

... observed in DU145 human prostatic carcinoma cells upon treatment with either phenylace- tate or phenylbutyrate, with the effect being larger in phenylbutyrate-treated cells, where a larger increase ... representa- tive experiment. Error bars indicate measurement errors. By comparing the results of at least seven inde- pendent experiments, the differences between control and i...

Ngày tải lên: 18/02/2014, 13:20

... (NEN Life Sciences, PE, Wal- tham, MA, USA). Dissected C. gigas mantle edges were fixed, dehydrated in an increasing alcohol series and xylene, and embedded in paraplast. Seven-micrometre sections ... compared with other sequences. The GH18 conserved sequence motif including the catalytic residues is marked with a thick black line above the sequence alignment. Arrowheads indicate...

Ngày tải lên: 19/02/2014, 00:20

... agarose gel and were stained with ethidium bromide. The sequences of the amplified fragments were determined as described above. Enzyme assay Enzyme activity of DNase I was determined by the single radial ... above. Plasmids The different length of 5¢-upstream sequence of exons 1 and 1a in the DNASE1 were PCR-amplified using sequence-spe- cific primers corresponding to the sequen...

Ngày tải lên: 19/02/2014, 06:20

... from one experiment with three replicates representative of at least three experiments; SE of the assays did not exceed 3% of the measured value. Acknowledgements This project was supported by the ... domain (capital letter in the sequence represent nucleotides match- ing target sequence). The PCR product was cloned into pET-32b plasmid (Novagen) deleted of the thioredoxin gene, u...

Ngày tải lên: 19/02/2014, 07:20

... environment with features that might be of commercial interest. The polar marine regions are characterized by their stabile low tempera- ture where the sea temperature rarely exceeds 4 °C. Enzymes ... multiple alignment of the C-terminal sequences ori- ginating from peptidase sequences of the proteinase K family along with some of the metallopeptidase sequences. Expression and p...

Ngày tải lên: 19/02/2014, 07:20

... hypothesis directly, Sno1p and Snz1p were separately expressed, purified and characterized as detailed under Experimental procedures. As described above, Sno1p expressed in E. coli resided in the inclusion body ... be emphasized that the dual mutation of the two genes was necessary to make yeast cells pyridoxine auxotrophic; cells were still viable in the absence of pyrid...

Ngày tải lên: 19/02/2014, 12:20