Báo cáo khoa học: Active site residues governing substrate selectivity and polyketide chain length in aloesone synthase docx

... compilation ª 2005 FEBS Active site residues governing substrate selectivity and polyketide chain length in aloesone synthase Ikuro Abe 1,2 , Tatsuya Watanabe 1 , Weiwei Lou 1 and Hiroshi Noguchi 1 1 School ... substitutions in place of the single resi- due lead to formation of shorter chain length products depending on the steric bulk of the side chain [18...

Ngày tải lên: 16/03/2014, 14:20

... these residues may be directly involved in binding of one of the substrates, the amino acid cysteine (Fig. 3). However, it cannot be excluded that cysteine binds to another site of CoaB and that ... conserved in CoaB proteins. Residues of the N-terminal part of the Escherichia coli CoaB domain that are conserved in CoaB (Dfp) proteins from all kingdoms of life and that were ex...

Ngày tải lên: 19/02/2014, 12:20

... this substrate and therefore the hydride transfer steps are not rate determining in this reaction. Site- directed mutagenesis In an effort to further understand the roles of the active site residues ... existence of an imine intermediate during catalysis, since the original C6¢¢ oxygen atom from UDP-glucose could conceivably be sequestered within the active site and redel...

Ngày tải lên: 23/03/2014, 12:20

... underlying substrate selectivity still remain to be discovered. With this in view, we examined the kinetic behaviour of pea seedling amine oxidase with cadaverine and hexylamine, the first bearing ... hydrophobic interactions. Instead, binding of the diamine is strongly facilitated by electrostatic factors, con- trolled by polar side-chains and two titratable residues present in...

Ngày tải lên: 06/03/2014, 00:21

... TK, reveal a common folding of these enzymes and in particular the amino acid residues involved in substrate interactions are highly conserved. However, the substrate binding site also exhibits some ... deoxyribonucleo- side kinase; dCK, deoxycytidine kinase; dGK, deoxyguanosine kinase; TK2, thymidine kinase 2; HSV-1 TK, herpes simplex virus type-1 thymidine kinase; dAdo, deoxya...

Ngày tải lên: 08/03/2014, 02:21

... a) 8 domain (amino acid resi- dues 32–330), and a C-terminal domain (residues 331– 429) containing eight antiparallel b strands on two sheets in a b sandwich (Fig. 1A) [28]. The first domain contains ... of protein folding in favor of proper folding, thereby increasing intracellular enzyme activity (Fig. 3). Retro- spectively, these enzyme inhibitors could be useful tools for probing a...

Ngày tải lên: 30/03/2014, 03:20

... didn’t understand the previous instruction; and handleStatusInformation gets called once per second and after each user action to inform the server of the player’s position and orientation and the ... player in a 3D game world with an NLG system over the Internet. It is imple- mented and open source, and can be a used online during EACL at www.give-challenge.org. In Section 2,...

Ngày tải lên: 22/02/2014, 02:20

... bind to a single PRE in vitro. This indicates that either the activation domain of Ste12 is incapable of activating transcription when bound to a single site, or that binding to a single site Organization ... examined the binding of recombinant maltose-binding domain-Ste12 fusions or Ste12 DNA-binding domain fragments to an FRE [15], or the FUS1 promoter in vitro [23]. We have expresse...

Ngày tải lên: 06/03/2014, 22:21

... binding K63-linked polyubiquitin chains than head to tail conjugated lin- ear polyubiquitin [21]. Because RIP1 is modified with predominantly K63-linked polyubiquitin chains [22], specific binding ... The NOA ⁄ UBAN ubiquitin-recognition domain is able to recognize linear ubiquitin linkages [20], but in the con- text of full -length NEMO a C-terminal ubiquitin bind- ing zinc finger in conju...

Ngày tải lên: 28/03/2014, 23:20

... and an antimycin-insensitive, myxothiazol-sensitive, quinol- cytochrome c oxidoreductase [1]. It also contains active membrane-bound NAD + -independent D -and L -lactate dehydrogenases ( D -and L -iLDH) ... iLDH expression in Euglena is independent of substrate availability and that iLDHs play a key role in the transfer of reducing equivalents from the cytosol to the res- pira...

Ngày tải lên: 30/03/2014, 20:20