Báo cáo khoa học: DNA mediated disassembly of hRad51 and hRad52 proteins and recruitment of hRad51 to ssDNA by hRad52 pot

... DNA mediated disassembly of hRad51 and hRad52 proteins and recruitment of hRad51 to ssDNA by hRad52 Vasundhara M. Navadgi, Ashish Shukla, Rahul Kumar Vempati and Basuthkar J. Rao Department of ... aggregation states of hRad51 and hRad52 in the presence of 121-mer ssDNA and hRad52 mediated assembly of the ssDNA hRad51 complex. The chang...

Ngày tải lên: 16/03/2014, 14:20

... signaling and loss of apoptosis [141]. Silencing of FASL and TRAIL-R1, TRAIL-R2 and Caspase-8 expression by DNA methylation has been linked to resistance of small cell lung cancer cells to FASL and ... dedicated (by SKP) to Dr MK Pal, retired professor of biochemistry, University of Kaly- ani and Dr D Chattopadhyay, professor of biochemis- try, molecular biolo...

Ngày tải lên: 18/02/2014, 14:20

... and HinfI for 3 h and recovered by ethanol precipitation. The precipitated DNA was dissolved in 3 lL of water and added into the ligation mixture [100 ng each of landmark- and HinfI-adapter and ... containing 5 lLof 10· PCR buffer (Takara, Tokyo, Japan), 4 lL of 2.5 mm each dNTP mixture, 10 pmol each of CasA- and CasB- specific primers, 1 lL of ligation solution, and...

Ngày tải lên: 19/02/2014, 02:20

... preparation of the p53 proteins, and Professor G. Melino for donation of plasmids pcDNA3-HA-p73b and pcDNA3-HA-p73d. This work was supported by IGA MH CR grant No. NC ⁄ 7574-3 and partly by GACR ... & Brabec V (2006) Recognition of DNA modified by trans-[PtCl2NH3(4-hydroxymethylpyridine)] by tumor suppressor protein p53 and character of DNA adducts of this cy...

Ngày tải lên: 19/02/2014, 05:20

... renaturation of single-strand DNA (ssDNA) and assimilation of ssDNA into homologous super-coiled duplex DNA. J Biol Chem 276, 41906–41912. 10 Nara T, Hamada F, Namekawa S & Sakaguchi K (2001) Strand ... single and double stranded DNA binding activity. Binding to single stranded DNA stimulated a significant level of DNA dependent ATPase activity. OsDmc1 also showed re...

Ngày tải lên: 19/02/2014, 07:20

... additional and innovative attention to clarify the mechanisms of action of this modulator when operating at distinct levels with respect to controlling the functionality and fate of this receptor. ... factor on binding of glycolytic enzymes to muscle cytoskeleton and the antagonistic action of calmodulin inhibitors. Biochem Med Metab Biol 50, 24–34. 107 Llado ´ A, Tebar...

Ngày tải lên: 06/03/2014, 09:22

... amplified and then cleaved with EcoRI. Restricted fragments A and B (to delete site I), C and D (to delete site II), E and F (to delete site III), G and H (to delete the perfect inverted repeat) and ... characterization of the DNA- binding properties in response to phosphoryla- tion by the sensorkinase SenS. Results Cloning of wild-type and mutant senS c and senR g...

Ngày tải lên: 07/03/2014, 10:20

... Academy of Finland to JES and HP and a grant by the German- Isareli Foundation for Scientific Research and Develop- ment to GK. We are grateful to Tomi Ma ¨ kela ¨ for generous gifts of antibodies and ... presence of radioactive UTP or dATP and the photoreactive DNA precursor BrdUTP, and subjected to UV crosslinking. The photolabelled derivatives of Pols a, d a...

Ngày tải lên: 07/03/2014, 11:20

... constitution of PTP1B fragments obtained by calpain -mediated cleavage in the absence of oxidation. Fig. S2. Detection of PTP1B in wild-type and calpain- deficient cells by immunocytochemistry. Fig. ... degradation of PTP1B by cal- pain in vitro in order to prove that calpain is responsi- ble for the cleavage reaction, to identify the site of cleavage and the mechanism...

Ngày tải lên: 16/03/2014, 00:20

... followed by chemiluminescence (ECL; Amersham) and autoradiography. Bands were quantified using a calibrated imaging densi- tometer (GS 710; Bio-Rad Laboratories) and analyzed using quantity one software ... levels of cytokines and cytokine receptors in patients with non-small cell lung cancer, and correlations with clinicopathological features and prognosis. M-CSF-an independent...

Ngày tải lên: 16/03/2014, 01:20