Báo cáo khoa học: Interaction of gymnemic acid with cyclodextrins analyzed by isothermal titration calorimetry, NMR and dynamic light scattering doc

... Interaction of gymnemic acid with cyclodextrins analyzed by isothermal titration calorimetry, NMR and dynamic light scattering Yusuke Izutani 1 , Kenji Kanaori 2 , Toshiaki Imoto 3 and Masayuki ... effect of gymnemic acid (GA) is diminished by application of c-cyclodextrin (c-CD) to the mouth was evaluated at the molecular level using isothermal...

Ngày tải lên: 16/03/2014, 14:20

... inactivation of PDE5A1 by caspase-3 might occur via removal of key regions which constitute part of the wall of the catalytic site of PDE5A1. We also suggest a possible interaction between PDE5A1 and ... Institute of Biological and Life Sciences, University of Glasgow, Scotland; 5 Department of Molecular Biology, Institute of Biotechnology, Flanders Interuniversity,...

Ngày tải lên: 17/03/2014, 09:20

... Food Chemistry and Toxicology, University of Karlsruhe, Germany; 2 Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland; 3 Institute of Food Technology and Food Chemistry, ... IIÞ where N ¼ the number of s trand breaks per molecule of PM2, I ¼ the p ercentage of supercoiled PM2 DNA, and II ¼ the percentage of open circular PM2 DNA. The...

Ngày tải lên: 30/03/2014, 15:20

... reconstituted with copper by mixing an aliquot of protein ( 10 mg) with an equal vol- ume of 10 mm Tris-HCl (pH 8), containing a three-fold molar excess of CuSO 4 in a final volume of 1 mL. The ... m )1 Æcm )1 . Trypsinization Trypsin digest of purified pro-tyrosinase was performed by dissolving 20 lg of proteomics grade TPCK treated porcine trypsin (Sigma-Aldrich) in 50 lL...

Ngày tải lên: 06/03/2014, 11:20

... HSP25 on the polymerization of intact actin and aggregation of heated actin. Interaction of HSP25 with intact actin In the first series of experiments we analyzed the effect of HSP25 on the apparent ... buffer G and was initiated by the addition of KCl and MgCl 2 up to 50 m M and 2 m M , respectively. Different quantities of actin nuclei (short fragments of F-ac...

Ngày tải lên: 20/02/2014, 23:20

... S3) [10,19]. Interactions of the truncated TnrA proteins with GlnK and with GS were determined by pulldown and SPR analysis, as described above (Fig. 6). As expected, the C-terminus of TnrA was ... deletion of even six amino acids abolished this interaction (Fig. 6A,B). Truncated forms of TnrA with the C-terminus lacking six or 20 amino acids still bound to GlnK; however...

Ngày tải lên: 06/03/2014, 00:21

... Shai Y & Jelinek R (2002) Structures and mode of mem- brane interaction of a short alpha helical lytic peptide and its diastereomer determined by NMR, FTIR, and fluorescence spectroscopy. Eur J ... of lipids, were recorded between 310 nm and 450 nm, with an excitation wavelength of 290 nm, at a slit width of 4 nm. The fluorescence spectra were instrument corrected f...

Ngày tải lên: 07/03/2014, 17:20

... oligomers of vesicles made of acidic phospholipids resembles pore formation by A-b [27] and liposome permeabilization of a-synuclein [43]. The toxic activity exerted by prefi- brillar forms of stefin B and ... by both transmis- sion electron microscopy (TEM) and atomic force microscopy [15]. In this study, we measured the interaction of stefin B with various combination...

Ngày tải lên: 07/03/2014, 17:20

... deletion of one, two and three pre- dicted helices from the N-terminus of MP reduced the interaction with NV by 51.5%, 66.4% and 80.1%, respectively, compared with the interaction between GST–MP and ... 4. Interaction of rMP with NV. (A) ELISA of rMP and NV inter- action. ELISA plates coated with NV (5 lg) (P1) were blocked with 10% skimmed milk in 1% NaCl ⁄...

Ngày tải lên: 15/03/2014, 00:20

... contaminant of eEF1A protein can be excluded by MALDI TOF analysis of the Coomassie blue band extract [21] and by the absence of a corresponding nucleolin signal in western-blotting of our nuclear extracts ... were washed twice with buffer C and twice with buffer C with- out NP40. The elution was made with three volumes of buffer C without NP40 adjusted to 1.5 m NaCl...

Ngày tải lên: 16/03/2014, 13:20