Báo cáo khoa học: Interaction of gymnemic acid with cyclodex

Báo cáo khoa học: Interaction of gymnemic acid with cyclodextrins analyzed by isothermal titration calorimetry, NMR and dynamic light scattering doc

... Interaction of gymnemic acid with cyclodextrins analyzed by isothermal titration calorimetry, NMR and dynamic light scattering Yusuke Izutani 1 , Kenji Kanaori 2 , Toshiaki Imoto 3 and Masayuki ... effect of gymnemic acid (GA) is diminished by application of c-cyclodextrin (c-CD) to the mouth was evaluated at the molecular level using isothermal...

Ngày tải lên : 16/03/2014, 14:20

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Báo cáo khoa học: Interaction of caspase-3 with the cyclic GMP binding cyclic GMP speciﬁc phosphodiesterase (PDE5a1) potx

... inactivation of PDE5A1 by caspase-3 might occur via removal of key regions which constitute part of the wall of the catalytic site of PDE5A1. We also suggest a possible interaction between PDE5A1 and ... Institute of Biological and Life Sciences, University of Glasgow, Scotland; 5 Department of Molecular Biology, Institute of Biotechnology, Flanders Interuniversity,...

Ngày tải lên : 17/03/2014, 09:20

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Báo cáo khoa học: Interaction of selenium compounds with zinc ﬁnger proteins involved in DNA repair pdf

... Food Chemistry and Toxicology, University of Karlsruhe, Germany; 2 Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland; 3 Institute of Food Technology and Food Chemistry, ... IIÞ where N ¼ the number of s trand breaks per molecule of PM2, I ¼ the p ercentage of supercoiled PM2 DNA, and II ¼ the percentage of open circular PM2 DNA. The...

Ngày tải lên : 30/03/2014, 15:20

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Báo cáo khoa học: Role of the C-terminal extension in a bacterial tyrosinase Michael Fairhead and Linda Thony-Meyer doc

... reconstituted with copper by mixing an aliquot of protein ( 10 mg) with an equal volume of 10 mm Tris-HCl (pH 8), containing a three-fold molar excess of CuSO 4 in a ﬁnal volume of 1 mL. The ... m )1 Æcm )1 . Trypsinization Trypsin digest of puriﬁed pro-tyrosinase was performed by dissolving 20 lg of proteomics grade TPCK treated porcine trypsin (Sigma-Aldrich) in 50 lL...

Ngày tải lên : 06/03/2014, 11:20

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Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc

... HSP25 on the polymerization of intact actin and aggregation of heated actin. Interaction of HSP25 with intact actin In the ﬁrst series of experiments we analyzed the effect of HSP25 on the apparent ... buffer G and was initiated by the addition of KCl and MgCl 2 up to 50 m M and 2 m M , respectively. Different quantities of actin nuclei (short fragments of F-ac...

Ngày tải lên : 20/02/2014, 23:20

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Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx

... S3) [10,19]. Interactions of the truncated TnrA proteins with GlnK and with GS were determined by pulldown and SPR analysis, as described above (Fig. 6). As expected, the C-terminus of TnrA was ... deletion of even six amino acids abolished this interaction (Fig. 6A,B). Truncated forms of TnrA with the C-terminus lacking six or 20 amino acids still bound to GlnK; however...

Ngày tải lên : 06/03/2014, 00:21

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Báo cáo khoa học: Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles doc

... Shai Y & Jelinek R (2002) Structures and mode of mem- brane interaction of a short alpha helical lytic peptide and its diastereomer determined by NMR, FTIR, and ﬂuorescence spectroscopy. Eur J ... of lipids, were recorded between 310 nm and 450 nm, with an excitation wavelength of 290 nm, at a slit width of 4 nm. The ﬂuorescence spectra were instrument corrected f...

Ngày tải lên : 07/03/2014, 17:20

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Báo cáo khoa học: Interaction of human steﬁn B in the preﬁbrillar oligomeric form with membranes Correlation with cellular toxicity doc

... oligomers of vesicles made of acidic phospholipids resembles pore formation by A-b [27] and liposome permeabilization of a-synuclein [43]. The toxic activity exerted by prefi- brillar forms of stefin B and ... by both transmis- sion electron microscopy (TEM) and atomic force microscopy [15]. In this study, we measured the interaction of stefin B with various combination...

Ngày tải lên : 07/03/2014, 17:20

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Báo cáo khoa học: Interaction of Sesbania mosaic virus movement protein with the coat protein – implications for viral spread Soumya Roy Chowdhury and Handanahal Subbarao Savithri docx

... deletion of one, two and three pre- dicted helices from the N-terminus of MP reduced the interaction with NV by 51.5%, 66.4% and 80.1%, respectively, compared with the interaction between GST–MP and ... 4. Interaction of rMP with NV. (A) ELISA of rMP and NV interaction. ELISA plates coated with NV (5 lg) (P1) were blocked with 10% skimmed milk in 1% NaCl ⁄...

Ngày tải lên : 15/03/2014, 00:20

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Báo cáo khoa học: Interaction of G-rich GT oligonucleotides with nuclearassociated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines potx

... contaminant of eEF1A protein can be excluded by MALDI TOF analysis of the Coomassie blue band extract [21] and by the absence of a corresponding nucleolin signal in western-blotting of our nuclear extracts ... were washed twice with buffer C and twice with buffer C without NP40. The elution was made with three volumes of buffer C without NP40 adjusted to 1.5 m NaCl...

Ngày tải lên : 16/03/2014, 13:20

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