Báo cáo khoa học: Intrabodies against the EVH1 domain of Wiskott–Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells potx
Báo cáo khoa học: Intrabodies against the EVH1 domain of Wiskott–Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells potx
... bind to the target proteins on the lumen side and inhibit transport of tar- get proteins in the process of functional maturation [6,8]. If the targets are cytosolic proteins, scFvs without the ... scFv Tg mice M. Sato et al. Intrabodies against the EVH1 domain of Wiskott–Aldrich syndrome protein inhibit T cell receptor signaling in transgenic...
Ngày tải lên: 16/03/2014, 14:20
Báo cáo khoa học: Zinc potentiates the antibacterial effects of histidine-rich peptides against Enterococcus faecalis pot
... activities of histatin 5 in the presence of ions Finally, we investigated the antibacterial effect of the histidin-rich peptide histatin 5 against E. faecalis 2374 in the presence of different ... demonstration that Zn 2+ potentiated the antimicrobial activity of a set of peptides from the heparin- and Zn 2+ -binding regions of HMWK domain 5 further substanti...
Ngày tải lên: 23/03/2014, 11:20
Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
... CCGGTG GTcGAcTTTGGTTtcACGCCC SalI E273Q ACGTACTGCTgTCCGGAA AGtACtCCATGGCCGC ScaI D353N TCCAATCCGTtAGTC TGgCCaTAGAACGCGC MscI E356Q GGAGAAT GGTgCCAGGGTACATTTcCAATCCGTCA KpnI D372N AATACAT CTTaAGGCCGTCGTtGTCGGTGTGG AflII D373N ... described in Materials and methods. Lower c ase letters indicate the nucleotide m utations. To facilitat e the selection of mutant clones, silent mutations wer...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: "Methods for the Qualitative Evaluation of Lexical Association Measures" doc
... computed. Another measure of goodness is the proportion of TPs in the base data that are also contained in the -best list (the re- call). While precision measures the quality of the -best lists ... significantly 10 different from the baseline obtained by random selection. In contrast to our expectation stated at the be- ginning of this section, the performance o...
Ngày tải lên: 20/02/2014, 18:20
Báo cáo khoa học: Global shape and pH stability of ovorubin, an oligomeric protein from the eggs of Pomacea canaliculata pot
... giving the best t to the scattering intensity. The resulting dummy atom model represents the shape of the scattering particle. To increase the reliability of the results, the final model for the ... number of predators. The pH stability of ovorubin is within the pH range of verte- brate digestive tract fluids [30,31], and the present results indicate that the...
Ngày tải lên: 07/03/2014, 06:20
Báo cáo khóa học: Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy pptx
... phenotype of B. pertussis,and for the translocation of the catalytic domain into the target cells [5–7]. The N-terminal domain of about 400 amino acids contains the CaM-dependent AC [2]. This domain ... observed in this region of the anthrax exotoxin on CaM addition [23]. In contrast with the relatively low sensitivity of the catalytic subdomain to CaM bi...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domain pptx
... partners. Karala and Ruddock [16] tested the effect of bacitra- cin on the reductive activity of a truncated PDI con- taining the catalytic a -domain, but lacking the independent substrate-binding ... pres- ence of the covalently bound bacitracin in this region would impair the binding of the substrate insulin, ulti- mately inhibiting its reduction. Although all bacitr...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khóa học: Acharan sulfate, the new glycosaminoglycan fromAchatina fulica Bowdich 1822 Structural heterogeneity, metabolic labeling and localization in the body, mucus and the organic shell matrix docx
... chain from the core protein) or papain digestion (except by the change in the profile of protein elution after treatment with this enzyme, as expected). However, we cannot exclude the possibility ... variants containing unsulfated iduronic acid. Acharan sulfate was found to be located in the body of this species using alcian blue staining and it was suggested to be the m...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo khoa học: Principles behind the multifarious control of signal transduction ERK phosphorylation and kinase/phosphatase control potx
... signaling amplitude, both in the initial phase of signaling and Fig. 2. Time profile of the activity of the four proteins in the model. The receptor R is inactivated (relatively) quickly to attain ... activity of a target molecule in tumor cells will affect cellular signaling, but in addition, the control that other kinases or phosphatases have on that sig...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Studies on the regulatory properties of the pterin cofactor and dopamine bound at the active site of human phenylalanine hydroxylase pptx
... to demonstrate the interactions of the dihydroxy- propyl side-chain of the pterin cofactors with the N-terminal autoregulatory sequence. Similarly, the coordinates for the structure of the binary ... [20] pterin cofactor onto the structure of the ligand- free rPAH (containing the regulatory and catalytic domains) [19] revealed that both the reduced and the oxid...
Ngày tải lên: 17/03/2014, 09:20