Báo cáo khoa học: Intrabodies against the EVH1 domain of Wiskott–Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells potx

... bind to the targetproteins on the lumen side and inhibit transport of tar-get proteins in the process of functional maturation[6,8]. If the targets are cytosolic proteins, scFvs without the ... scFv Tg mice M. Sato et al. Intrabodies against the EVH1 domain of Wiskott–Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells Mitsuru Sato1, Ryo Iwaya1,2, Kazumasa ... association of WASP and the Arp2 ⁄ 3 complex activates the actinnucleation activity of the Arp2 ⁄ 3 complex [23].To investigate further the function of the WASP- EVH1 domain in the TCR signaling pathway,...

Báo cáo khoa học: Zinc potentiates the antibacterial effects of histidine-rich peptides against Enterococcus faecalis pot

... activities of histatin 5 in the presence of ionsFinally, we investigated the antibacterial effect of the histidin-rich peptide histatin 5 against E. faecalis 2374 in the presence of different ... demonstration that Zn2+potentiated the antimicrobial activity of a set of peptides from the heparin- and Zn2+-binding regions of HMWK domain 5 further substantiates ﬁndings obtained with the prototypic ... Zn2+mayregulate the antimicrobial activity of histidine-richAMPs. In this context, it is interesting to note that the total concentration of Zn2+ in plasma is 10–18 lm,but thrombocytes can accumulate...

Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc

... CCGGTGGTcGAcTTTGGTTtcACGCCCSalIE273Q ACGTACTGCTgTCCGGAAAGtACtCCATGGCCGCScaID353N TCCAATCCGTtAGTCTGgCCaTAGAACGCGCMscIE356Q GGAGAATGGTgCCAGGGTACATTTcCAATCCGTCAKpnID372N AATACATCTTaAGGCCGTCGTtGTCGGTGTGGAﬂIID373N ... described in Materials and methods.Lower c ase letters indicate the nucleotide m utations. To facilitat e the selection of mutant clones, silent mutations were made to introducerestriction enzyme ... panose (data notshown).Prediction of the positions of amino acid residues whosesubstitutions resulted in the reduction of the IPU activityWhile the study of site-directed mutagenesis describedabove...

Tài liệu Báo cáo khoa học: "Methods for the Qualitative Evaluation of Lexical Association Measures" doc

... computed. Another measure of goodness is the proportion of TPs in the base datathat are also contained in the -best list (the re-call). While precision measures the quality of the -best lists ... signiﬁcantly10differentfrom the baseline obtained by random selection. In contrast to our expectation stated at the be-ginning of this section, the performance of and relative to the other AMs is not better forhigh-frequency ... log-likelihood and t- test lead to the best re-sults, with log-likelihood giving an overall betterresult than the t- test. The picture differs slightlyfor the PNV data (Figure 2). Here t- test outper-forms...

Báo cáo khoa học: Global shape and pH stability of ovorubin, an oligomeric protein from the eggs of Pomacea canaliculata pot

... giving the best t to the scattering intensity. The resulting dummy atom model represents the shape of the scattering particle. To increase the reliability of the results, the ﬁnal model for the ... number of predators. The pHstability of ovorubin is within the pH range of verte-brate digestive tract ﬂuids [30,31], and the presentresults indicate that the protein can withstand the com-bined ... subunits,leading to their dissociation. In addition, the isoelectric point determined at pH 4.9 suggests thatalterations in the charge of the molecule are takingpart in the Rgchange. All these results...

Báo cáo khóa học: Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using ﬂuorescence spectroscopy pptx

... phenotype of B. pertussis,andfor the translocation of the catalytic domain into the target cells [5–7]. The N-terminal domain of about 400 aminoacids contains the CaM-dependent AC [2]. This domain ... observed in this region of the anthrax exotoxin on CaM addition [23]. In contrast with the relatively low sensitivity of the catalytic subdomain to CaM binding, at least indicatedusing the two probes ... site as probedby Ant-dATPTo explore the dynamics of the catalytic domain of the protein, we used the ﬂuorescent nucleotide Ant-dATP, astrong competitive inhibitor of AC activity [22]. Binding...

Báo cáo khoa học: Bacitracin inhibits the reductive activity of protein disulﬁde isomerase by disulﬁde bond formation with free cysteines in the substrate-binding domain pptx

... partners.Karala and Ruddock [16] tested the effect of bacitra-cin on the reductive activity of a truncated PDI con-taining the catalytic a -domain, but lacking the independent substrate-binding ... pres-ence of the covalently bound bacitracin in this regionwould impair the binding of the substrate insulin, ulti-mately inhibiting its reduction. Although all bacitracinanalogs seem to interact ... not diminish the immunoblot signals for bacitracin at the site of PDI (Fig. 4C). These data support the view of a robustcovalent PDI–bacitracin interaction.We hypothesized that the thiazoline...

Báo cáo khóa học: Acharan sulfate, the new glycosaminoglycan fromAchatina fulica Bowdich 1822 Structural heterogeneity, metabolic labeling and localization in the body, mucus and the organic shell matrix docx

... chain from the core protein) or papaindigestion (except by the change in the proﬁle of protein elution after treatment with this enzyme, as expected).However, we cannot exclude the possibility ... variants containingunsulfated iduronic acid. Acharan sulfate was found to belocated in the body of this species using alcian blue stainingand it was suggested to be the main constituent of the ... column. Fractions were monitored by scintillation counting (k). The NaCl concentration in the fractions (–) was determined by measuring the conductivity. The fractions corresponding to the 35S-labeled...

Báo cáo khoa học: Principles behind the multifarious control of signal transduction ERK phosphorylation and kinase/phosphatase control potx

... signaling amplitude, both in the initial phase of signaling andFig. 2. Time proﬁle of the activity of the four proteins in the model. The receptor R is inactivated (relatively) quickly to attain ... activity of a target molecule in tumor cells will affect cellular signaling, but in addition, the control that other kinases or phosphatases have onthat signaling. Therefore, antitumor strategies ... value. If at that point in time the time deriv-ative is small, then the simpler form of the law isretained. We now consider the time t it takes for the concentration to attain a certain valuey...

Báo cáo khoa học: Studies on the regulatory properties of the pterin cofactor and dopamine bound at the active site of human phenylalanine hydroxylase pptx

... to demonstrate the interactions of the dihydroxy-propyl side-chain of the pterin cofactors with the N-terminalautoregulatory sequence. Similarly, the coordinates for the structure of the binary ... [20] pterin cofactor onto the structure of the ligand-free rPAH (containing the regulatory and catalytic domains)[19] revealed that both the reduced and the oxidized cofactorinteract with the ... onto that of the ligand-free rPAH containing the regulatory andcatalytic domains [19] the catecholamine main-chain is alsoFig. 4. Stereo view of the site of interactionbetween the pterin cofactors...

Xem thêm