Báo cáo khoa học: Substrate specificity of vaccinia virus thymidylate kinase docx
Báo cáo khoa học: Substrate specificity of vaccinia virus thymidylate kinase docx
... cidofovir have already been described [5].
Vaccinia virus, the prototype member of the Orthopox-
virus family, is most often used as a surrogate virus for
variola virus. Unlike most other DNA viruses, ... virus, a DNA arbovirus.
We describe here the cloning and expression in
E. coli of the vaccinia virus TMP kinase gene. We
characterized for the first time the specificity...
Tài liệu Báo cáo khoa học: Substrate specificity of the pseudouridine synthase RluD in Escherichia coli doc
... to the RluA family [2]. Binding of
RluA to one of its substrates, tRNA
Phe
anticodon
stem-loop, induces reorganization of the RNA [21]. An
ability of the RNA substrate to adopt the alternative
fold ... RluD
has low substrate specificity, converting one out of 20
uridine residues in 23S rRNA and one out of eight uri-
dine residues in 16S rRNA to pseudouridine [9].
We have analy...
Báo cáo khoa học: Substrate specificity of the human UDP-glucuronosyltransferase UGT2B4 and UGT2B7 Identification of a critical aromatic amino acid residue at position 33 doc
... UGT2B4 may play an important role in the
substrate specificity of the isoform.
Importance of amino acid residue tyrosine 33
in the substrate specificity of UGT2B7
The wild-type UGT2B7 efficiently ... in UGT2B13 did not change the
specificity of this isoform.
The aim of this study was to identify amino acid res-
idues that are involved in substrate specificity of
UGTs 2B4...
Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt
... determined from the slope ()E
a
⁄ R) of Arrhenius
plots of log V (nmol of product per minute) versus
1 ⁄ T.
Substrate specificity
The substrate specificity of BHs was determined using
the synthetic ... with
55 mL of the same buffer, first alone and then with a
200 mL of a decreasing gradient of ammonium sul-
phate in 20 mm Tris⁄ HCl buffer (pH 8.0) with a flow
of 0.8 mLÆmi...
Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx
... striking in the case
Fig. 1. Substrate specificity of mPAT2. (A,B) Representative current traces of oocytes expressing mP AT2 in response to perfusion with 20 m
M
of
the different substrates in Na
+
-free ... substrate i n t he range
of 100–700 l
M
, whereas those of PAT1 are in the range of
1–15 m
M
[2,7,9,10]. PAT1 recognizes, besides
L
-a amino
acids, a number of addition...
Báo cáo khoa học: Substrate specificity and excision kinetics of natural polymorphic variants and phosphomimetic mutants of human 8-oxoguanine-DNA glycosylase pot
... distant from DNA.
Activity and substrate specificity of OGG1
mutants on oligodeoxynucleotide substrates
OGG1 is part of an enzymatic system responsible for
prevention of mutations generated by 8-oxoGua ... enzyme (Table 3).
The C ⁄ A specificity for all phosphomimetic mutants of
OGG1 resembled closely that of the wild-type enzyme
(Table 3).
Activity and substrate specificity...
Báo cáo khoa học: Site specificity of yeast histone acetyltransferase B complex in vivo pot
... as substrates of the two peptides is due to
different positions being modified by the enzyme. Our
data indicate a site specificity of the yeast HAT-B
complex that exactly matches the specificity of ... investigating the in vivo specificity of
the yeast HAT-B complex, and found that incubation
of cells with hydroxyurea (HU) resulted in an increase
of the histone H4 isoform with a...
Báo cáo khoa học: Aglycone specificity of Escherichia coli a-xylosidase investigated by transxylosylation ppt
... Acceptor
specificity and regioselectivity were investigated using various sugars as
acceptor substrates and a-xylosyl fluoride as the donor substrate. Compari-
son of the rate of formation of the ... studying the specificity of hydrolytic activity, thus
avoiding the synthesis of many different oligosaccha-
ride substrates.
Previously, we reported that the functionally
unknown prot...
Báo cáo khoa học: The specificity of alcohol dehydrogenase with cis-retinoids Activity with 11-cis-retinol and localization in retina docx
... an estimate of the effect of thecis-
bond position on the substrate specificity of human ADH4.
The 7-cis-and9-cis-retinol and retinal isomers were the most
active substrates, in terms of k
cat
/K
m
,forADH4,followedby
11-cis-retinol ... iterative cycles,
allowing free movement of the rotatable bonds of the
substrate and of the v angles of the residues inside a 5 A
˚
radiu...
Báo cáo Y học: Substrate specificity of human kallikrein 2 (hK2) as determined by phage display technology docx
... selection of specific enzyme
substrates [13,15,25].
The determination of the specificity constants of the
substrates showed a positive tendency during the selection.
Most of the better substrates ... percentage of hydrolysis, the specificity
constant and the site of cleavage (11).
Ó FEBS 2002 Substrate specificity of kallikrein hK2 (Eur. J. Biochem. 269) 2749
nitrilotriaceti...