Báo cáo khoa học: Reconstitution in vitro of the GDP-fucose biosynthetic pathways of Caenorhabditis elegans and Drosophila melanogaster ppt

... 2006 The Authors Journal compilation ª 2006 FEBS Reconstitution in vitro of the GDP-fucose biosynthetic pathways of Caenorhabditis elegans and Drosophila melanogaster Simone Rhomberg1, Christina ... Amsterdam, the Netherlands), which weregrown overnight in 10 mL Luria–Bertani medium containingkanamycin and chloramphenicol (also containing ampicillin in the case of double transformation). In the ... (alternatively spliced form of GMD-1), gmd-2 and ger-1. The amounts of cDNA used in the PCRs were normalized on the basis of the intensity of actin transcripts. GDP-fucose biosynthesis in invertebrates S....

Báo cáo khoa học: "Conundrums in Noun Phrase Coreference Resolution: Making Sense of the State-of-the-Art" docx

... runs using anoptimal threshold (box 3) for the experiment as de-termined by using the test set. In all remaining ex-periments, we learn the threshold from the trainingset as in the BASELINE ... including the number of documents, annotated CEs, coreference chains, annotatedCEs per chain (average), and number of documents in the train/test split. We use st to indicate a standard train/test ... coreference chain to which ce isassigned in the response (i.e. the system-generatedoutput) and Kceis the coreference chain that con-tains ce in the key (i.e. the gold standard). Pre-cision and recall...

Báo cáo khoa học: "Proximity in Context: an empirically grounded computational model of proximity for processing topological spatial expressions∗" docx

... location of other objects in the scene. Ourmodel is based on ﬁrst computing absolute prox-imity between each point and each landmark in ascene, and then combining or overlaying the re-sulting ... each point in the scene comparing the ab-solute proximity of the selected landmark at thatpoint with the absolute proximity of all other land-marks at that point. The relative proximity of aselected ... relativeproximity than the other. There are two such areas in the Figure. The ﬁrst is between the two land-marks, in the region where one relative proxim-ity ﬁeld line crosses the other. These points areambiguous...

Tài liệu Báo cáo khoa học: Kinetic basis for linking the ﬁrst two enzymes of chlorophyll biosynthesis doc

... depletion of the putative intermediatewas followed by integrating the peaks at 12.7 min on the HPLCchromatograms for each timepoint. The data for the decay of inter-mediate in the presence of ChlH ... reaction intermediate, resulting in a reduction in the lag phase of product accumulation, and an increase in initial rate of product evolution. Furthermore, the presence of ChlH increases the magnitude ... [14]. The current value is a better estimate of intermediateaccumulation, as the ﬁt in Fig. 3B considers only the evolution of intermediate. The rate constant for the decay of intermediate in the...

Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc

... blotting of modiﬁed proteinsWe identiﬁed the major proteins in the mitochondrialmatrix by performing SDS/PAGE separation and staining the gel with Coomassie blue or Western blotting. Analysis of ... decrease in the amount of this protein in the matrix. We have not detected any signiﬁcant modiﬁca-tion in the amount of this protein in the matrix. We canconclude that the decrease in this enzymatic ... with two intense signals of 70 and 50 kDa, while the bands at 60 and 150 kDavanished.With oxyblot (Fig. 4C), antibodies stained carbonylatedproteins mainly in band ranges of 30–60 and 70–120...

Báo cáo khoa học: Small peptides derived from the Lys active fragment of the mung bean trypsin inhibitor are fully active against trypsin pptx

... unit of BBI main-taining inhibitory activity.A B Fig. 6. Inhibition curves of the native MBTI, Lys33GP, the Lys16peptide and the mutant Lys33K ⁄ F against bovine trypsin (A) and against bovine ... expression of the intact MBTI was unsuccessfulbecause of mispairing of its seven disulﬁde bonds, and the inhibitory activity of the recombinant onlyaccounted for 1 ⁄ 10 of the activity of the native ... describe the cDNA cloning of MBTI, and the gene expression of a 35-residuepeptide and its mutant both derived from the long chain of the Lys active fragment of MBTI. The total synthesis of a 16-residue...

Báo cáo khoa học: "A New Approach to the Mechanical Syntactic Analysis of Russian" ppt

... satellites has the position number 3097 in the 47th block on the 2nd tape. To α we attach the tag t and intersort the result with the other contents of the sorting file. The entry in the internal ... found in the glossary, the TCj are obtained by finding the common intersection between the possibilities given by its ending in the Table of Endings and those given by the morphological information ... found in the list of pseudo-roots. In that case, the transliteration subroutine dictates the form of the correspondent to be stored in the normal position of the target T for the final printout....

Báo cáo khoa học: "A Clustering Approach for the Nearly Unsupervised Recognition of Nonliteral Language" pdf

... legalstraits of the institute.N4 She had a hand in his fully comprehending the quandary. The target set consists of sentences from the corpus containing the target word. The feedbacksets contain sentences ... features are shown in bold.Target Set1 The girl and her brother grasped their mother’s hand.2 He thinks he has grasped the essentials of the institute’sﬁnance philosophies.3 The president failed ... provided in the target-word en-tries of these datasets. TroFi attempts to cluster the target set sentences into literal and nonliteral byattracting them to the corresponding feature setsusing Algorithms...

Báo cáo khoa học: "Optimization in Coreference Resolution Is Not Needed: A Nearly-Optimal Algorithm with Intensional Constraints" ppt

... whileadhering to the global constraints. One of the central global constraints in the ﬁeld of corefer-ence resolution evolves from the interplay of intra-sentential binding constraints and the transitivity of ... this lies in the logical na-ture of NLP constraints. For example in the case of coreference, we have the following types of con-straints:1. exclusivity of two instantiations (e.g. eithercoreferent ... applied in the linear variant as well, so the onlydifference is the ordering. Linear ordering overpairs is established by sorting according to the in- dex of the ﬁrst pair element (the i from...

Tài liệu Báo cáo khoa học: Speciﬁc interaction between the classical swine fever virus NS5B protein and the viral genome pdf

... (EMSA) and R dRpassays, and examined the in uence of d ifferent cis-actingelements on binding of NS5B protein and RNA synthesis in vitro. Materials and methodsExpression and puriﬁcation of NS5B ... have the b inding activity,indicating that the CSFV NS5B p rotein binds the 3¢ UTRmainly through interaction with the s everal nucleotide basesat the t erminal of the 3¢ UTR. Therefore, the ... tobinding of the CSFV NS5B protein. The minus-strand3¢ UTR with deletion of the 21 nucleotide fragment at the terminal did not interact with the N S5B protein at all.Characterization of the cis-acting...

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